Experiences with Surgical Reconstruction of Hepatic Jejunal Anastomosis in Rats - Abstract
Objectives: Based on establishing the animal model of hepatic jejunal anastomosis, the degree of anastomosis solidity, histological characteristics, postoperative complications were investigated after hepatic jejunal anastomosis. Methods: SPF SD rats were randomly divided into 4 groups (6 in each group). Liver function was detected at the 1st, 7th, and 14th days after surgery. The volume of bleeding, infection, anastomotic leakage, and the bursting pressure was measured in the 7th day and 14th day after operation. Histological examination was performed to assess the pathological morphological changes of the anastomosis. Results: All animals survived well and there were no abnormalities in appetite and activity. There was no significant difference in AST and ALT. Complications such as infection, bleeding, anastomotic leakage, and intestinal obstruction were not observed. The anastomotic healing was good. Histological examination showed that the surface of the intestinal mucosa villi epithelium was intact, and the intestinal wall serous layer was adhered to the hepatic lobule. Lymphocyte infiltration, granulation tissue and many new capillary blood vessels can be observed. Conclusion: Hepatic jejunal anastomosis has sufficient healing fastness, which could be used as the new biliary reconstruction method is used in clinic to make up for the past deficiencies.