Expression Analysis of COX2 in Patients Suffering from Esophageal Squamous Cell Carcinoma - Abstract
Objective: To investigate the expression of COX-2 gene in patients suffering from esophageal squamous cell carcinoma
Study design: Descriptive Place and duration of study: Institute of Basic Medical Sciences, Khyber Medical University
Patients and methods: A total of 69 patients, of esophageal squamous cell carcinoma (ESCC), comprising of Pakistanis and Afghans were enrolled. Various risk factors associated with ESCC were recorded. Immunohistochemical (IHC) expression of COX-2 was determined in normal esophageal mucosa, Carcinoma in situ (CIS) and invasive ESCC. Differences of mean were computed with ANOVA test followed by Post Hoc test. Patients were categorized as positive with high expression or negative with low to nil expression.
Results: Annova test showed large differences in expression of COX-2 in normal healthy mucosa compared to CIS tissue and ESCC with the mean difference of -9.529 and -7.370 respectively, p-value <.05 at 95% confidence interval (CI). No significant difference was noticed in expression of COX-2 in CIS compared to ESCC with the p-value >.05 at 95% CI. Mean age at diagnosis was 55 years. Out of 69 patients of ESCC in our sample, 46 (67%) were users of Naswar.
Conclusion: The cohort, 23-85 years shows statistically significant difference in expression of COX-2 gene in ESCC and CIS tissue sample compared to normal healthy mucosa. Over-expression of COX-2 is positively associated with ESCC