Liver is one of the vital organs of the body. Fatty liver is an excessive amassing of fats in the liver which causes anorexia, reduced milk production, recumbency, death and impairs the normal liver functions which is evident by the rise in level of liver enzymes. Conventional treatment has many side effects so the herbal medicines as an alternative therapy is grabbing the attention of the researchers. The present study aims to use Withania somnifera, a herbal plant rich in phytochemicals and possesses many benefits. In this study, rat model of fatty liver was used. There were four groups of rats and each group will have n=6. Group 1 was the the control group and fed with normal chow diet, group 2 was the negative control fed with High fat diet (HFD), group 3 was the positive control and group 4 was the treatment group for 12 weeks. Moreover, trial was terminated at 12th week and liver as well as kidney biomarkers were evaluated along with the histological analysis to see the effects induced by Withania somnifera. The herbal treatment depicted its beneficial hepatoprotective effects along with a betterment in biomarkers of liver and kidney.