Muscle tears are a common injury with limited treatment options that effectively accelerate healing. This case study explores the use of a Protein-Rich Platelet-Rich Plasma Matrix (PR-PRP), as a multipurpose biologic platform for treating acute muscle tears in two athletes of significantly different ages: a 19-year-old D1 intercollegiate female soccer player with a quadriceps - rectus femoris tear, and a 73-year-old recreational male tennis player with a hamstring - biceps femoris tear. Both individuals received a single injection of Protein-Rich PRP Matrix, delivered to the individual injury sites to enhance tissue regeneration through the sustained release of growth factors, chemokines, leukocytes, and provide a scaffold that supports cell migration, followed by a structured rehabilitation exercise program. Post treatment assessments and musculoskeletal ultrasound imaging demonstrated accelerated recovery, reduced pain, and enhanced tissue regeneration in both patients. These findings highlight the potential of Protein-Rich PRP matrices in acute muscle injuries regardless of the patient’s age.