Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by varying degrees of impairments in language, social interaction, and behavior. Acupuncture has been proven to be an effective and safe treatment. We aim to provide an overview of the clinical research status and efficacy of different acupuncture protocols in the treatment of ASD, systematically review and critically evaluate the existing evidence on the effectiveness and safety of acupuncture in children with ASD. We searched the randomized controlled trials (RCTs) of acupuncture for children with ASD in databases such as China National Knowledge Infrastructure (CNKI), PubMed, and Chinese Biology Medicine disc (CBMdisc) etc. in the past decade, conducted a retrospective summary and evaluated the efficacy of different acupuncture protocols. By observing the changes in the scores of relevant scales before and after treatment, all studies found that both the single utilization and comprehensive utilization of acupuncture therapy have certain clinical efficacy in improving ASD children’s physical and activity participation-related functions such as language, social interaction, and behavioral patterns, and also has efficacy in alleviating comorbidities. This review suggests that acupuncture has certain clinical efficacy in alleviating ASD children’s core symptoms. However, Due to statistical and clinical heterogeneity among studies, it can’t determine whether there’re therapeutic discrepancies between different acupuncture protocols.