Background: Otitis media (OM) is among the most common infections in childhood. Approximately 60% of all children will experience one or more episodes of OM before the age of 7, and 26% of children in Denmark are treated with Ventilation Tube Insertion (VTI) before the age of 7. Previous studies investigating the effects of VTI on quality of life (QoL) for children and caregivers have yielded conflicting results. Here, we investigate whether OM and VTI affect the QoL in children and their families in rural setting of Denmark. Methods: From the LOFUS cohort, caregivers of 1.169 children aged 2-10 years, answered questions on how OM affects the children’s and their family’s general QoL. Additionally, we used Visual Analogue Scales from zero (no effect) to 10 (worst possible effect) to score the impact on QoL. Results: In 480 children (43%) were not reported any episodes of OM, 385 (35%) children had 1-3 episodes and 247 (22%) children had >3 episodes. Twenty-six percent of the total childhood cohort and 47% of children who reported OM had had VTI procedure performed. We found an Odds Ratio (OR) of 1.54 (0.36-10.62) and 2.66 (0.62-18.41) for poorer QoL in the affected child and an OR of 1.13 (0.26-7.91) and 1.91 (0.43-13.34) for poorer QoL in the family, for 1-3 and >3 episodes, respectively. VTI yielded an OR of 2.13 (95% CI 1.48-3.08, p<0.001) and 2.27 (95% CI 1.54-3.37, p<0.001) for poorer QoL on children’s and family’s QoL, respectively. Conclusion: Our study shows a high frequency of reported OM and a high frequency of VTI procedures performed in a rural population in Denmark. Furthermore, the study shows an association correlation between VTI and poorer QoL in the children and in their families.