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  • ISSN: 2373-9436
    Volume 5, Issue 2
    Research Article
    Ayse Kubra Karaboga Arslan*, Ebru Qzturk, and Mukerrem Betul Yerer
    Lung adenocarcinoma is one of the most commonly occurring cancer types and it is the leading cause of cancer-related deaths worldwide. Vanadium compounds have different pharmacological effects and have cytotoxic properties. Aim of this study was by continuous monitoring to assay the cytotoxicity of vanadium pentoxide (V2O5) on human lung carcinoma cell line (A549) and human bronchial epithelial cell line (Beas-2b). Eight different concentrations of V2O5 between 2.5-40µM were applied on the cells and xCELLigence Real Time Cell Analysis (RTCA) was conducted to evaluate the impedance alterations over the Cell index values. Our results suggest the idea that V2O5 causes toxicity both on A549 and Beas-2b. We observed a dose-dependent cytotoxicity at 15 µM and higher doses in the A549 cells which might reveal its anticancer metallodrug potential. However, for Beas-2b cells although the cytotoxicity of V2O5 started after 5 µM, after 10 µM, the CI alterations reached a stable value at all doses applied, resulting in a maximum reduction of 50% in this healthy cells. Therefore these results revealed us that, 20 µM V2O5 at which cytotoxicity is initiated or up to 40 µM at which the IC50 level have been reached in A549 can also be used in Beas-2b since the maximum toxicity have been already reached at/over 10µM. We demonstrated that in cytotoxicity assays, the xCELLigence system can be used to optimize parameters such as the exposure time and compound concentrations. This study is the known first study to show V2O5’s effects at these concentrations on A549 and Beas-2b in a real-time manner.
    Jaikanth C, Indhumathi T, Prema Gurumurthy*, and Cherian KM
    Background: Recently, HMG-CoA reductase inhibitors are receiving larger importance in cancer therapeutics as they targets both metabolic and signal transduction platform. However, their functional role in targeting Triple negative breast cancer cells and its associated mechanism remains elusive.
    Methods: In this study, growth inhibitory activity of simvastatin on MDA-MB-468 cells was assessed by MTT assay and its apoptotic potential by Nexin staining. Further, we employed label free quantitative proteomic profiling using mass tandem spectroscopy to explore the differentially expressed proteins associated with its anticancer activity.
    Results: Treatment of MDA-MB-468 cells with increased concentrations of Simvastatin showed a remarkable growth inhibitory activity with the IC50 value of 9 µg/ml. Further, Nexin staining of the treated cells clearly indicates that, Simvastatin induces apoptosis in MDA-MD-468 cells. In Label free quantitative proteomic profiling of Simvastatin treated and untreated cells, 74 differentially expressed proteins were identified of which, 43 were up regulated and 31 were down regulated. Gene ontology and KEGG pathway enrichment analysis exposed 18 potential pathways associated with Simvastatin treatment. These identified pathways were shown to be related with focal adhesion, tumor progression, metastasis and metabolic effects in cancer cells. Among the down regulated proteins HSP90-alpha, Filamin-A, Alpha actinin-4, Vimentin and Phosphoglycerate kinase 1 was significantly down regulated.
    Conclusion: These results imply that the application of Simvastatin is a possible new drug in the field of neoplasia to control growth and progression of breast carcinoma cells. Further our proteomic profiling reveals potential new drug targets for future drug development.
    Seung Chan Kim* and Beom Jin Kim
    Osteopontin (OPN) is a protein associated with several cellular processes, including development, carcinogenesis, and adhesion. The U87 and U251 glioblastoma multiforme cell line is a model for fast-growing malignant tumors. Taking advantage of the relatively fast growing speed of this cell line, we investigated the subcellular relocalization of proteins as an indicator of time-dependent effects following a treatment with static magnetic field (2000 ± 600 Gauss). OPN, which plays an important role in adhesion and mitosis, was transported from the perinucleus to the cytoplasm upon static magnetic stimulation (p = 0.0217). Similarly, tubulin gamma complex associated protein 3 (GCP3) was dispersed. Tunneling electron microscopy indicated that the membranous structures had changed. Our findings suggest that static magnetic fields induce the dispersion of cytoplasmic intracellular proteins such as OPN and GCP3.
    Menha Swellam*, Magda Sayed Mahmoud, and Aly Khatab Mohamed
    Objectives: Authors aimed to investigate the association between GSTT1 and GSTM1 genetic polymorphism and susceptibility to acute myeloid leukemia (AML).
    Materials and methods: Genotyping polymorphisms were studied in a total adult 178 individuals (88 newly diagnosed AML patients and 90 healthy controls) using multiplex polymerase chain reaction (PCR).
    Results: Significant difference was reported between genotype polymorphism in AML as compared to control individuals. High risk incidence was detected when dual null of the candidate genotypes were combined (p < 0.0001).
    Conclusion: Dual combination of the null GSTT1 and GSTM1 were superior to the susceptibility to leukemogenesis.
    Mini Review
    Muhammad Torequl Islam*
    Context: Uraria lagopodies DC. (Fabaceae) is currently used as a traditional medicinal plant in many small communities of Bangladesh, India, Africa and other countries in the world.
    Objective: This text offers a review on U. lagopodies.
    Methods: A search was made in the electronic databases such as PubMed, Science Direct and Google scholar for the published articles till January 31, 2017.
    Results and conclusion: The findings suggest that the plant is rich in flavonoids and glycosides and it possesses a number of important biological activities, including antioxidant, anti-inflammatory, antimicrobial, cytotoxic, anti-diarrheal, analgesic and others. U. lagopodies may be a good contributor of plant-based modern medicines.
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