Purpose: Dry eye disease (DED), is a chronic condition affect ocular surface and tears. We intended to evaluate the efficacy of autologous platelet rich plasma (PRP), topical eye drops in chronic dry eye diseases. Methods: A prospective study that included 40 eyes from 20 patients with symptomatic Dry eye at Outpatient clinic of Department of Ophthalmology, Benha University Hospitals, Egypt. All patients included in the study were subjected to full history taking and the following ophthalmic examination (before and after treatment), including: Slit lamp examination, Schirmer test, Tear film break up time (TBUT), Corneal fluorescein staining (CFS), Best spectacle corrected visual acuity (BCVA), and A questionnaire of Ocular Surface Disease Index (OSDI). The patients underwent these examinations before the beginning of the treatment with autologous platelet rich plasma (PRP), topical eye drops (5 times a day per eye), and then the examinations were repeated after one month of the utilization of (PRP), topical eye drops. Results: The mean (SD), of ODSI score before treatment was higher as compared with the ODSI score after treatment. The mean (SD), of value of Schirmer, TF-BUT and corneal fluorescein staining test before treatment was lower as compared with the values after treatment with high statistically significantly difference between the two values in both eyes (P< 0.001). Regarding visual acuity after treatment by platelet rich plasma, in the right eyes, there were 6 eyes with Gain 1 or more line while 14 eyes showed no gain in visual acuity. Also, in the left eyes, there were 6 eyes with Gain 1 or more line while 14 eyes showed no gain in visual acuity Conclusion: A major reduction in symptoms and various parameters of dry eye disease is consistent with PRP eye drops.