Effect of Standardized Isoflavones Rich Soya Seed Extract on Glucose Utilization and Endurance Capacity in Type- II Diabetic Mice - Abstract
Soya (Glycine max (L) Merr) isoflavones have the property to enhance mitochondrial
biogenesis and improve the status of diabetes mellitus. Isoflavones rich soya seed
extract (DRE) was standardized for daidzin using HPLC. In the present investigation, DRE
(16.22 % w/w daidzin) was tested for its endurance capacity and glucose utilization
in high fat diet and multiple low dose streptozotocin induced type-2 diabetic mice. DRE
(140 mg/kg, p.o.), Metformin (10 mg/kg, p.o.) treatment and exercise were induced
for a period of 28 days. The biochemical parameters, endurance capacity, glucose
tolerance, utilization and muscle antioxidant level were measured at terminal. All the
parameters were compared with diabetic control. DRE has showed significant (p<0.01)
improvement in endurance capacity and metabolic flexibility which was reflected in
improved muscle performance, glucose utilization in oral glucose tolerance test and
insulin tolerance test. However, the exercise induced mice showed the best improvement
in the endurance performance. Marked improvement in fasting plasma glucose, TG
and TC was observed in DRE treated mice in comparison to diabetic control. Muscle
antioxidant activity was measured by estimating SOD, catalase and MDA levels. DRE
and Metformin treatment have showed significant (p<0.01) free radical scavenging
activity compared to diabetic control. Moreover, the animals treated with DRE and
Metformin combination demonstrated more potent activity in OGTT, ITT, fasting plasma
glucose, TG level. Similar effect was also observed in skeletal muscle catalase and
MDA activity. DRE treatment has significantly improved diabetic condition however, the
combination of DRE and Metformin showed better efficacy and can be a potential
therapeutic option.