Objective: This study aims to determine the efficacy of Pelvic Floor Muscle Training (PFMT) and lifestyle improvement versus combined biofeedback electrical stimulation-intensive PFMT in ordinary women with pelvic organ prolapse by detecting electrophysiological indicators of pelvic floor muscles. Methods: A retrospective analysis was conducted on 437 women who visited our outpatient department for gynecological examinations due to conscious vaginal prolapse between May 2020 and September 2022. According to the Glazer protocol, general patient information was collected, and pelvic floor function was evaluated using pelvic floor surface electromyography. Results: The experimental group showed significant differences in other evaluation values six months after the intervention (P < 0.05), except for mean amplitude variability in the rest pre-baseline stage (P = 0.067) and in the endurance contracts stage (P = 0.147). The control group showed significant improvements in all other indicators, except for the mean amplitude variability in the rest pre-baseline stage (P = 0.876). The average peak amplitude of phasic (flick) contractions and the average mean amplitude of tonic contractions in the experimental group improved significantly compared to those in the control group (P = 0.016 and 0.004, respectively). Conclusion: Although PFMT can improve the condition of Pelvic Floor Muscles (PFMs), a combination of basic PFMT and biofeedback electrical stimulation therapy is more effective. Although the normal standard has not been achieved, the training duration of PFMs can be extended to achieve better results.