Objective: To investigate the effect of acupoint application in treating stable Chronic Obstructive Pulmonary Disease (COPD) patients and the effect on Th17, Treg and Th17/Treg expression and the improvement of lung function in. Methods: A total of 100 patients with stable COPD admitted to Qiaotou Town Health Center, Datong County, Xining City, Qinghai Province from January to June in 2024 who were 1:1 randomly divided into experimental group and control group. The control group received conventional treatment, and the observation group received conventional treatment combined with acupoint application. Both groups received continuous treatment for 4 weeks. Th17, Treg, Th17/Treg expression, lung function, and CAT scores were compared between the two groups. Results: (1) After treatment, Th17, Th17/Treg were lower than before treatment (P < 0.001), and Treg were higher than before treatment (P < 0.05). After treatment, Th17, Th17/Treg and Treg in the experimental group were better than those in the control group (P < 0.05). (2) The levels of IL-17 after treatment were lower than before treatment (P < 0.05), and the levels of TGF-? were higher than before treatment (P < 0.05). After treatment, the expression in the experimental group was better than that of the control group (P < 0.05). (3) Pulmonary function FEV1 and FEV1/FVC were significantly improved in the two groups after treatment (P < 0.001), and the improvement was more significant in the experimental group (P < 0.05). (4) After treatment, the CAT evaluation scores in both groups were decreased (P < 0.05), and the treatment group was more significant (P < 0.05). Conclusion: Acupoint application combined with routine treatment in stable COPD patients has better efficacy, and can improve cellular immune function and lung function.