Extraction, Purification, and Biological Evaluation of Flavonoids from Spirulina CPCC695 - Abstract
Flavonoids are low molecular weight plant secondary metabolites having polyphenolic structure and are associated with plant cell stress tolerance, antioxidant properties and detoxification potential. Recently, flavonoids have gained commercial applications in the field of nutraceutical, pharmaceutical, medicinal, and cosmetic industries. This has compelled the scientist to look for good, high yielding, low cost bioresources. Therefore, in present study Spirulina CPCC695 flavonoid was investigated for its bioactivities. HPLC-MS analysis of the flavonoid revealed 2 prominent peaks, identified as quercetin and rutin. The purified flavonoid depicted satisfactory ?-amylase and ?-glucosidase inhibition activity with IC50 = 112.43 and 130.68 µg/ml, respectively along with considerable anti-inflammatory activity (IC50 = 40.14 ± 0.14 µg/ml). Among DPPH, ABTS, NOR and PM antioxidant activity the sample showed highest activity in ABTS assay (IC50 = 40.12 ± 0.10 µg/ml). Inhibition of hemolysis and thrombosis (1.80% and 81.46%) with 250 ?g/ml suggested its safe use for human consumption. The findings of the present study suggested that Spirulina CPCC-695 may be used as a promising aquatic renewable source of flavonoids for pharmaceutical and nutraceutical applications.