Genome-Wide Microsatellite Survey and Introgression of Null KTI Allele in Charcoal Rot Resistant Soybean - Abstract
Soybean though being a vegetable crop have not been used for table purpose due to various quality constraints. Kunitz trypsin inhibitor is a major ANF in soybean which interfere protein digestion and absorption. The objective of the experiment is formulated to address these issues with soybean that are preventing high-quality soybean production is necessary. For parental polymorphism, 388 SSR markers across all 20 linkage groups were examined in four parental combinations comprising four soybean genotypes: AMS MB-5-18, AMS MB-5-19, NRC 101, and NRC 127. Seventy-eight molecular markers showed polymorphism with 37 of them being highly polymorphic. Allelic frequency of 3.84, 4.3, 4.7 and 3.95 per marker was observed respectively for parental combinations 1,2,3 and 4 PIC value ranging from 0.11 to 0.75, 0.15 to 0.75, 0.25 to 0.75 and 0.13 to 0.75 respectively. Using a marker specific to the Null KTI gene, foreground selection was applied to the F1 progenies acquired from the experiment. Future soybean breeding programs will benefit from the obtained polymorphic markers.