Loading

JSM Dentistry

Biofilm Formation on Dental Materials in the Presence of Khat: Review

Review Article | Open Access

  • 1. Department of Prosthetic Dental Science, Jazan University, Saudi Arabia
  • 2. Department of Preventive Dental Science, Jazan University, Saudi Arabia
  • 3. Department of Prosthodontics, Aleppo University, Syria
+ Show More - Show Less
Corresponding Authors
Mohammed M Al Moaleem, Department of Prosthetic Dental Science, College of Dentistry, Jazan University, Saudi Arabia
Abstract

Microbiological composition in the oral cavity is affected by components and shape of restorative materials used. Consequently, such composition may affect oral health and restorative materials. Secondary caries form in teeth that are partly restored with restorative materials. This condition is a common dental disease caused by bacterial biofilms and with unknown causes. Caries are related to the type of restoration material used. In relation to biomaterials, several factors, such as surface roughness, surface energy, and chemical composition, affect Microbiota composition and biofilm formation. Ceramic and dental alloys have resulted in fewer caries formation, whereas composites cause more secondary caries than amalgam or glass ionomers. Khat chewing in the Arabian Peninsula is associated with a range of orodental problems. This paper provides an overview of scientific literature regarding the association among properties and performances of different restorative materials and oral biofilm formation in the presence of khat. PubMed literatures published until June 2016 were researched using the following keywords: ceramic, alloy, denture materials, composite resin, amalgam, biofilm, khat. Bibliographies of available previous reviews and their cross references were manually searched.

Keywords


•    Biofilm
•    Composite
•    Amalgam
•    Ceramic
•    Alloy
•    Resin
•    Khat

Citation

Al Moaleem MM, Dorout IA, Elamin EF, Mattoo KA, Ghazali NAL (2017) Biofilm Formation on Dental Materials in the Presence of Khat: Review. JSM Dent 5(2): 1087.

INTRODUCTION

The oral cavity is constantly contaminated by a complex diversity of microbial species that exhibit strong tendency to colonize dental surfaces, the tongue, and oral mucosa. The main components in biofilm formation comprise bacterial cells, a hard surface, and a fluid medium [1,2].

Formation of biofilms on intra and extra coronal teeth surfaces primarily causes periodontal diseases and caries [3]. A multitude of biomaterials used for restoration also cause such oral conditions [4].

Biofilm formation on restorative materials may degrade the material and roughen its surface [5], thereby causing filling of bacteria in the interface between tooth structure and restorative material and formation of secondary caries [6] and affecting pulp pathology [7].

Recovery of aesthetic and masticatory functions requires the use of proper dental restorative materials. However, these materials are prone to biofilm formation, thereby affecting oral health. In general, under clinical conditions, rough surfaces form more biofilm than smooth ones, but factors affecting bacterial adhesion to new restorative dental material remain unclear and may result in increased synthesis of antimicrobial compounds [8,9].

Halbach [10], Luqman and Danowski [11] reported that long-term khat chewing causes stomatitis followed by secondary infection. This finding may be caused by chemical irritation of mucosal surfaces and mechanical strain on cheeks and other oral tissues. Low prevalence of dental caries and high rate of periodontal pocket depth and diseases have been reported among khat chewers [11].

Recently, the effect of khat on oral bacteria has been assessed in a series of studies. In vitro experiments showed that crude khat extracts interfere with biofilm formation by Streptococcus mutans, suggesting their anticariogenic properties [12]. In another study, extracts exhibited selective antimicrobial properties against major periodontal pathogens [13] and were found to foster growth of some health-compatible species [14]. The present paper aims to highlight the association between physical and mechanical properties of restorative and prosthetic dental materials and oral biofilm formation in the presence of khat

THE BIOFILM FORMATION

Dental biofilms are matrix-enclosed bacterial population adherent to each other and/or to surfaces, including polished tooth surfaces, living tissues, prosthetics devices, and dental materials. These films provide colonizing species with advantages, such as protection from competing microorganisms, environmental factors, host defense, and toxic substances. In the oral cavity, dental biofilm comprises diverse microorganisms; more than 500 different cultivable bacterial species are indigenous to the human oral cavity [15,16]. Currently, more than 700 oral bacterial taxa have been identified [17]. Among these organisms, approximately 100 or fewer species normally inhabit the oral cavity of an individual [18]. Although whole saliva features no distinctive microbiota of its own [19], it harbors as much as 108 bacteria per 1 mL [20] and serves as reservoir of microorganisms regularly derived from dental plaque biofilms adhering to gingival crevices, periodontal pockets, the dorsum of the tongue, and other oral mucosal surfaces [21]. Using only sequence analysis of previously characterized 16S rDNA and a number of previously uncultured and uncharacterized ones, bacterial species have recently been identified in saliva of healthy individuals and patients with periodontitis [22]. Mutans streptococci and lactobacilli have been associated with etiology of dental caries. Among mutans streptococci, Streptococcus mutans and S. sobrinus are considered particularly significant in human caries [23].

BIOFILM FORMATION AND KHAT

Khat is the leaves of the shrub (Catha edulis Forks) which are widely spread, chewed, consumed, and practiced by a majority of the youth in Jazan southwest (Saudi Arabia) [24,25]. Khat chewed like tobacco or used to make tea daily or during social and cultural gatherings and held in the lower buccal pouch unilaterally in a bolus for more than 5 hours or more [26,27]. Khat was reported to cause dental attrition, staining of teeth, TMJ disorders (pain and clicking), and cervical caries particularly among crystallized sugar consumers, and increased periodontal problems and attachment loss [28].

An in vitro effect of crude khat extracts on oral micro-organisms and the effect of bacteria identified from sub and supragingival plaques. Al-Hebshi et al. [14], demonstrated a possible antimicrobial effect of khat on oral micro-organisms and showed a selective antimicrobial effect of crude khat extracts on oral micro-organisms. They demonstrated that while bacteria associated with periodontal disease were sensitive to the extracts, bacteria associated with periodontal health were less sensitive, and cariogenic bacteria were not susceptible. In another study, Al-Hebshi et al. [12], showed that crude khat extracts interfered with the ability of Streptococcus mutans to form adherent biofilms, implying that khat may have anticariogenic effects.

Nyanchoka et al. [29], founded a significantly higher caries rate in khat chewers than in non-chewers, as measured by the decayed, missing and filled teeth (DMFT) index. They found the mean DMFT score were 8.778 and 6.529 for chewers and who never chewed khat respectively. The authors suggested that the higher caries index score in chewers could be a result of cariogenic substances such as soft drinks that are often consumed with khat [29,30]. While Hattab and Al-Abdulla [26] noticed that, Khat leaves contain a negligible amount of fluoride and thus is unlikely to exert anti-caries effect as claimed previously.

Regarding the effect of khat chewing on oral micro-organisms, the available evidence consistently indicates that chewing khat did not favor the proliferation of pathogenic oral micro-organisms. It was shown to have selective antimicrobial effects and to favor the presence of micro-organisms compatible with oral health [31,32]. A studies by [31-33], concluded that Khat chewers shown periodontal health adverse outcomes such as, gingival recession or bleeding and periodontal pocketing comparing to non-chewers, with effect sizes ranging from medium to large. It has also been shown that chewing is associated with other indicators of periodontal health and tooth loss [34].

BIOFILM FORMATION AND PROSTHETIC MATERIALS

Prosthetic materials may affect accumulation of biofilm in different ways. Rough or open margins consistently form between tooth and prosthesis, and this condition may complicate mechanical removal of biofilms and alter chemical balance in biofilm in this region.

Ceramics

The use of dental porcelain is advocated in different types of restorations like veneers, inlays, single crowns and fixed partial dentures [35]. Studies both in-vitro [36,37] and in-vivo [36,38-42] have investigated the adhesion of bacterial and bacterial biofilms on ceramics in comparison to other dental materials. Relatively and in comparison to other dental materials used in oral cavity, ceramics have been found to promote lower bacterial adhesion and biofilm formation although very less in vivo studies have been conducted to study the differences between different types of ceramics [43,44]. Variation between different ceramics has been studied in vitro for determination of bacterial adhesion rather than the accumulation of complex biofilms [44]. Ceramic surfaces have been shown to collect less plaque with reduced viability in absence of oral hygiene although different results have been demonstrated when compared with unglazed porcelain surface [45,46].

Acrylic resins

Since 1928, denture base resins are a group of dental materials that have stood the test of time without undergoing much change in its basic constituents. Biofilm associated with denture base resins is unique in the sense that more than bacteria, it is certain yeasts especially candida species that have been associated with denture base resins [47-49]. Many different strains of candida [50] along with certain bacteria [51] have been shown to work synergistically for their attachment to denture base resin or to each other [49, 52,53].

Biofilm including yeasts has been found to be difficult to remove because of strong adhesion ability, [54] the adhesive ability is directly associated with micoporous surface of denture resins [55-57].

Modification of resins to discourage biofilm formation in the form of polyethylene [54], titanium dioxides coating [58] and denture cleansers [59] have shown to discourage the biofilm formation.

Metal alloys

Alloys used for prostheses should be inert and highly polished to prevent the accumulation and attraction of oral microorganism to prevent biofilm formation. Different alloys used in dentistry mainly gold, nickel chrome and recently titanium alloys. Prostheses alloys margin with many small defects will retain more plaque and bacteria than a smooth margin. Most alloys should be polished to give very little retention for biofilms, although some alloys have a higher affinity to bacteria than others [60]. It seems that some bacteria are attracted due to electrical charges in some alloys [8].

Biofilms on gold restorations, however, generally have low viability [60]. Some microbes are affected by elutes from the metals. Auschill et al. [60], demonstrated that oral biofilms have very low viability (less than 2%) on gold but this cannot be due to the release of toxic compounds, because gold is completely inert. They explained that possibly, full coverage by a relatively thick oral biofilm hampers the supply of nutrients to the biofilm, leading to low viability [61].

Biofilm formations and restorative materials

Dental restorations affect biofilm composition in different ways. Steps, open margins, or groves consistently form between tooth and restorative materials. These spaces will complicate mechanical removal of biofilms and alter chemical balance in the biofilm in this region. Restorative materials differ from enamel with regard to surface roughness, surface energy, and chemical composition [62,63]. Most populations receive at least one dental restoration, and roles of biofilm-related infections to restoration as opposed to primary oral infections are not easily distinguished.

Amalgam

This material cannot bond to the tooth structures, so it depends manly on macro-mechanical undercuts for their retention. This resulted in interfacial spaces which lead to secondary caries [64]. Since amalgam is a conducting material, like gold so, electron transfer plays a role in bacterial adhesion [65]. This is attributed this to attraction between the negatively charged bacteria and their conducting material positive image charges [66]. Auschill et al. [60], ring biofilm on amalgam and gold and found that five-day-old oral biofilms on their surfaces were thick and fully covering the sub-stratum surfaces [60]. Leonhardt et al. [67], placed different restorative materials in teeth for day and 3days, he showed that amalgam attracted about 50% of viable bacteria than titanium oxide [67,68]. They explained the low viability of biofilms on amalgam surfaces is may be due to the release of toxic compounds from the alloy. However, it is possible that bacteria develop resistance against mercury because of instant bacteriostatic effects of it [60]. Experimentally more bacteria resistant to mercury were found in microcosm oral biofilms grown on amalgam than on enamel. The percentage and levels of this mercury resist bacteria remained elevated for a period of 2 days, but after that it returned to baseline levels [68].

Composite resins

Surface deterioration of resin composites has been demonstrated by an increased roughness, effects on filler particle exposure, and sometimes by a reduced micro-hardness of the materials upon exposure to biofilms in vitro [5]. Clearly, the clinical presence of biofilm is just one of the factors that may stimulate surface degradation, other factors being acidic fluid intake, temperature fluctuations, or simply the presence of an aqueous environment.

Some methods to inhibit biofilm growth on dental material are such as blended the zinc oxide nanoparticles into resin composites and or adding of chlorhexidinegluconate into some dental materials in order to enhance the antibacterial activity and display antimicrobial activity and reduce growth of bacterial biofilms [69-71]. In addition to that development of a nanocomposite containing amorphous calcium phosphate or calcium fluoride nanoparticles and chlorhexidinegluconate particles might be reduced biofilm formation Cheng et al. [72].

The removal of filler particles based on the roughness dimensions created. Resin composites with larger 0.01 to 3.5 μm filler particles became significantly less rough (around 15 nm) after biofilm growth [5]. Recently Khalichi et al. [73], found that triethylene glycol, as the ether portion of triethylene glycol dimethacrylate, modulates the expression levels of glucosyltransferase B involved in biofilm formation and yfiV as a putative transcription regulator gene in S. mutans. Glass-ionomer fillings Biofilm formation on glass-ionomer cements leads to a negative spiral of events [5], in which the colonizing organisms cause severe deterioration of the surface, which, in turn, promotes biofilm formation and therewith more extensive deterioration of the surface. The clinical manifestation of this downward spiral is the development of caries around or below a restoration [74].

The use of glass-ionomer potentially reduces micro leakage by adhering to tooth structure and enhances fluoride release with a potential impact on oral biofilm formation. Fluoride release occurs through an initially high burst release that may be between 1.6 and 1.8 μg/ mm 2, after which a prolonged, longterm tail-release follows [75].

Fluoride can act as a buffer to neutralize acids produced by bacteria and reduced the growth of caries related oral bacteria [76,77]. Glass-ionomer cement indeed collects a thin biofilm with a low viability (2% to 3%), possibly as a result of fluoride release [60]. However, an in vitro study also showed that glass-ionomer containing fluoride did not reduce the amount of bacterial growth and biofilm formation on the surfaces bathed in saliva [78]. This suggests that either fluoride is not a dominant factor in controlling biofilm formation, or the too low concentration to be effective, depending on the ratio between filling area and fluid volume in which the experiments were carried out. In the oral cavity, the large volume of saliva present, which is subject to wash out, makes the build-up of an effective fluoride concentration difficult [75].

Association of biofilm and khat on prosthetic and restorative materials

No study explored the relation of prosthetic materials and biofilm among khat chewers or the relation of khat and restorative materials. Little information is available on the pattern of dental biofilm distribution on different Prosthodontics and restorative materials. However, no literature or clinical and laboratory studies demonstrate the relationship between biofilm formation and khat chewers.

 

DISCUSSION AND CONCLUSION

In vitro and in vivo studies reveal that rough surfaces will promote plaque maturation and formation on restorative materials. Thick biofilms form on metal alloys and amalgam, but thin ones are more common in ceramic and glass ionomer restorations. Khat chewing has been shown to modify compositions of supra- and subgingival microbes, leading to periodontal recession, pocketing, and attachment loss of teeth. Case control and high-powered cohort studies bear significance in investigating the association among biofilm formations, restorative materials, khat chewing, and dental health. Finally, the present review should be considered for further clinical studies.

ACKNOWLEDGEMENTS

This review article was supported by a grant fund from the 7th Research Program number 37/ 7/ 00168, Deanship of Scientific Research, College of Dentistry, Jazan University

REFERENCES

1. Gharechahi M, Moosavi H, Forghani M. Effect of surface roughness and materials composition on biofilm formation. J Biomaterials Nanobiotechnology. 2012; 3: 541-546.

2. Marsh PD. Contemporary perspective on plaque control. Br Dent J. 2012; 212: 601-606.

3. Sbordone L, Bortolaia C. Oral microbial biofilms and plaque-related diseases: microbial communities and their role in the shift from oral health to disease. Clin Oral Investig. 2003; 7: 181-188.

4. Grossner-Schreiber B, Teichmann J, Hannig M, Dorfer C, Wenderoth DF, Ott SJ. Modified implant surfaces show different biofilm compositions under in vivo conditions. Clin Oral Implants Res. 2009; 20: 817-826.

5. Beyth N, Bahir R, Matalon S, Domb AJ, Weiss EI. Streptococcus mutans biofilm changes surface-topography of resin composites. Dent Mater. 2008; 24: 732-736.

6. Collins CJ, Bryant RW, Hodge KL. A clinical evaluation of posterior composite resin restorations: 8-year findings. J Dent. 1998; 26: 311- 317.

7. Pashley DH. Clinical considerations of microleakage. J Endod. 1990; 16: 70-77.

8. Busscher HJ, Rinastiti M, Siswomihardjo W, van der Mei HC. Biofilm formation on dental restorative and implant materials. J Dent Res. 2010; 89: 657-665.

9. Fernandes JMFA, et al. Improving Antimicrobial Activity of Dental Restorative Materials. Emerging Trends in Oral Health Sciences and Dentistry. 2015.

10. Halbach H. Medical aspects of the chewing of khat leaves. Bull World Health Organ. 1972; 47: 21-29.

11. Luqman W, Danowski TS. The use of khat (Catha edulis) in Yemen. Social and medical observations. Ann Intern Med. 1976; 85: 246-249.

12. Al-Hebshi NN, Nielsen O, Skaug N. In vitro effects of crude khat extracts on the growth, colonization, and glucosyltransferases of Streptococcus mutans. Acta Odontol Scand. 2005; 63: 136-142.

13. Al-hebshi N, Al-haroni M, Skaug N. In vitro antimicrobial and resistance-modifying activities of aqueous crude khat extracts against oral microorganisms. Arch Oral Biol. 2006; 51: 183-188.

14. Al-Hebshi N. Khat and oral microbiota - a study with relevance to periodontitis and dental caries University of Bergen. 2002.

15. Moore WE, Burmeister JA, Brooks CN, Ranney RR, Hinkelmann KH, Schieken RM, et al. Investigation of the influences of puberty, genetics, and environment on the composition of subgingival periodontal floras. Infect Immun. 1993; 61: 2891-2898.

16. Haffajee AD, Socransky SS, Feres M, Ximènez-Fyvie LA. Plaque microbiology in health and disease. In: Newman HN and Wilson M (eds) dental plaque revisited oral biofilms in health and disease Bioline/ UK; 1999. 255-282.

17. Kolenbrander PE. Oral microbial communities: biofilms, interactions, and genetic systems. Annu Rev Microbiol. 2000; 54: 413-437.

18. Consensus Report. Periodontal diseases: pathogenesis and microbial factors. Ann Periodontol 1996; 1: 929-932.

19. Beighton D. The value of salivary bacterial counts in the prediction of caries activity. In: Johnson, NW. Risk markers for oral diseases. Cambridge: Beighton. 1991; 313-326.

20. Marsh PD. Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res. 1994; 8: 263-271. 21.Van der Velden U, Van Winkelhoff AJ, Abbas F, De Graaff J. The habitat of periodontopathic micro-organisms. J Clin Periodontol. 1986; 13: 243-248.

22. Sakamoto M, Umeda M, Ishikawa I, Benno Y. Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA libraries. Microbiol Immunol. 2000; 44: 643-652. 23.Van Houte J. Microbiological predictors of caries risk. Adv Dent Res. 1993; 7: 87-96.

24. Sheikh KA, El-Setouhy M, Yagoub U, Alsanosy R, Ahmed Z. Khat chewing and health related quality of life: cross-sectional study in Jazan region, Kingdom of Saudi Arabia. Health Qual Life Outcomes. 2014; 12.

25. Ageely HM. Prevalence of Khat chewing in college and secondary (high) school students of Jazan region, Saudi Arabia. Harm Reduct J. 2009; 6.

26. Hattab FN, Al-Abdulla N. Effect of Khat Chewing on General and Oral Health. J Oral Medi. 2011; 7: 33-35.

27. Imran AG, Murad AH. The effect of Khat chewing on periodontal tissues and buccal mucosa membrane. Damascus Univ Med Sci J. 2009; 25: 493-504.

28. Hassan NA, Gunaid AA, Murray-Lyon IM. Khat (Catha edulis): health aspects of khat chewing. East Mediterr Health J. 2007; 13: 706-718.

29. Nyanchoka IN, Dimba EAO, Chindia ML, Wanzala P, Macigo FG. The oral and dental effects of khat chewing in the Eastleigh area of Nairobi. J Kenya Dent Asso. 2008; 1: 37-42.

30. Al-Sharabi AKK. Conditions of oral mucosa due to takhzeen al-qat. Yemeni J Medil Sci. 2011; 5: 1-6.

31. Al-Hebshi NN, Al-Sharabi AK, Shuga-Aldin HM, Al-Haroni M and Ghandour I. Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. J Ethnopharmacol. 2010; 132: 564-569.

32. Al-Hebshi NN, Skaug N. Effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population. Oral Microbiol Immunol. 2005; 20: 141-146.

33. Al-Kholani AI. Influence of Khat Chewing on Periodontal Tissues and Oral Hygiene Status among Yemenis. Dent Res J (Isfahan). 2010; 7: 1-6.

34. Al-Bayaty FH, Ali NAW, Bulgiba AM, Masood M, Hussain SF, Abdulla MA. Tooth mortality in khat and non khat chewer in Sana’a Yemen. Sc Res Essays. 2011; 6: 1039-1045.

35. Nakamura K, Kanno T, Milleding P, Ortengren U. Zirconia as a dental implant abutment material: a systematic review. Int J Prosthodont. 2010; 23: 299-309.

36. Rimondini L, Cerroni L, Carrassi A, Torricelli P. Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study. Int J Oral Maxillofac Implants. 2002; 17: 793-798.

37. Rosentritt M, Hahnel S, Gröger G, Mühlfriedel B, Bürgers R, Handel G. Adhesion of Streptococcus mutans to various dental materials in a laminar flow chamber system. J Biomed Mater Res B Appl Biomater. 2008; 86: 36-44.

38. Eick S, Glockmann E, Brandl B, Pfister W. Adherence of Streptococcus mutans to various restorative materials in a continuous flow system. J Oral Rehabil. 2004; 31: 278-285.

39. Kantorski KZ, Scotti R, Valandro LF, Bottino MA, Koga-Ito CY, Jorge AO. Surface roughness and bacterial adherence to resin composites and ceramics. Oral Health Prev Dent. 2009; 7: 29-32.

40. Tanner J, Robinson C, Söderling E, Vallittu P. Early plaque formation on fibre-reinforced composites in vivo. Clin Oral Investig. 2005; 9: 154-160.

41. Scarano A, Piattelli M, Caputi S, Favero GA, Piattelli A. Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study. J Periodontol. 2004; 75: 292-296.

42. Auschill TM, Arweiler NB, Brecx M, Reich E, Sculean A, Netuschil L. The effect of dental restorative materials on dental biofilm. Eur J Oral Sci. 2002; 110: 48-53.

43. Hahnel S, Rosentritt M, Handel G, Bürgers R. Surface characterization of dental ceramics and initial streptococcal adhesion in vitro. Dent Mater. 2009; 25: 969-975.

44. Rosentritt M, Behr M, Thaller C, Rudolph H, Feilzer A. Fracture performance of computer-aided manufactured zirconia and alloy crowns. Quintessence Int. 2009; 40: 655-662.

45. Hahn R, Weiger R, Netuschil L, Brüch M. Microbial accumulation and vitality on different restorative materials. Dent Mater. 1993; 9: 312- 316.

46. Bremer F, Grade S, Kohorst P, Stiesch M. In vivo biofilm formation on different dental ceramics. Quintessence Int. 2011; 42: 565-574.

47. Powers JM, Sakaguchi RL. Craig’s restorative dental materials. 12th edn. USA: Mosby Elsevier. 2006.

48. Ramage G, Tomsett K, Wickes BL, López-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 98: 53-59.

49. Verran J, Motteram KL. The effect of adherent oral streptococci on the subsequent adherence of Candida albicans to acrylic in vitro. J Dent. 1987; 15: 73-76.

50. Koopmans AS, Kippuw N, de Graaff J. Bacterial involvement in dentureinduced stomatitis. J Dent Res. 1988; 67: 1246-1250.

51. Coco BJ, Bagg J, Cross LJ, Jose A, Cross J, Ramage G. Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. Oral Microbiol Immunol. 2008; 23: 377-383.

52. Avon SL, Goulet JP, Deslauriers N. Removable acrylic resin disk as a sampling system for the study of denture biofilms in vivo. J Prosthet Dent. 2007; 97: 32-38.

53. Bamford CV, d’Mello A, Nobbs AH, Dutton LC, Vickerman MM, Jenkinson HF. Streptococcus gordonii modulates Candida albicans biofilm formation through intergeneric communication. Infect Immun 2009; 77: 3696-3704.

54. Chandra J, Patel JD, Li J, Zhou G, Mukherjee PK, McCormick TS, et al. Modification of surface properties of biomaterials influences the ability of Candida albicans to form biofilms. Appl Environ Microbiol. 2005; 71: 8795-8801.

55. Branting C, Sund ML, Linder LE. The influence of Streptococcus mutans on adhesion of Candida albicans to acrylic surfaces in vitro. Arch Oral Biol. 1989; 34: 347-353.

56. Edgerton M, Scannapieco FA, Reddy MS, Levine MJ. Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Infect Immun. 1993; 61: 2644- 2652.

57. Samaranayake LP, MacFarlane TW. An in-vitro study of the adherence of Candida albicans to acrylic surfaces. Arch Oral Biol. 1980; 25: 603- 609.

58. Arai T, Ueda T, Sugiyama T, Sakurai K. Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating. J Oral Rehabil. 2009; 36: 902-908.

59. da Silva PM, Acosta EJ, Pinto Lde R, Graeff M, Spolidorio DM, Almeida RS, et al. Micro-scopical Analysis of Candida albicans Biofilms on HeatPolymerised Acrylic Resin after Chlorhexidine Gluconate and Sodium Hypochlorite Treatments. Mycoses. 2011; 54: 712-717.

60. Auschill TM, Arweiler NB, Brecx M, Reich E, Sculean A, Netuschil L. The effect of dental restorative materials on dental biofilm. Eur J Oral Sci. 2002; 110: 48-53.

61. Ong CT, Ivanovski S, Needleman IG, Retzepi M, Moles DR, Tonetti MS, et al. Systematic review of implant outcomes in treated periodontitis subjects. J Clin Periodontol. 2008; 35: 438-462.

62. Adamczyk E, Spiechowicz E. Plaque accumulation on crowns made of various materials. Int J Prosthodont. 1990; 3: 285-291.

63. Chan C, Weber H. Plaque retention on teeth restored with full-ceramic crowns: a comparative study. J Prosthet Dent. 1986; 56: 666-671.

64. Ozer F, Unlü N, Oztürk B, Sengun A. Amalgam repair: evaluation of bond strength and microleakage. Oper Dent. 2002; 27: 199-203.

65. Poortinga AT, Bos R, Busscher HJ. Measurement of charge transfer during bacterial adhesion to an indium tin oxide surface in a parallel plate flow chamber. J Microbiol Methods. 1999; 38: 183-189.

66. Mei L, van der Mei HC, Ren Y, Norde W, Busscher HJ. Poisson analysis of streptococcal bond strengthening on stainless steel with and without a salivary conditioning film. Langmuir. 2009; 25: 6227-6231.

67. Leonhardt A, Olsson J, Dahlén G. Bacterial colonization on titanium, hydroxyapatite, and amalgam surfaces in vivo. J Dent Res. 1995; 74: 1607-1612.

68. Ready D, Pratten J, Mordan N, Watts E, Wilson M. The effect of amalgam exposure on mercury- and antibiotic-resistant bacteria. Int J Antimicrob Agents. 2007; 30: 34-39.

69. AydinSevinç B, Hanley L. Antibacterial Activity of Dental Composites Containing Zinc Oxide Nanoparticles. J Biomed Mater Res B Appl Biomater. 2010; 94: 22-31.

70. Hasan Zarrabi M, Javidi M, Naderinasab M, Gharechahi M. Comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and Portland. J Oral Sci. 2009; 51: 437-442.

71. Bidar M, Naderinasab M, Talati A, Ghazvini K, As-gary S, Hadizadeh B, et al. The Effect of Different Concentrations of Chlor-hexidine Gluconate on the Antimicrobial Properties of Mineral Trioxide Aggregate and Calcium Enrich Mixture. Dent Rese J. 2012; 9: 466-471.

72. Cheng L, Weir MD, Xu HH, Kraigsley AM, Lin NJ, Lin-Gibson S, et al. Antibacterial and physical properties of calcium-phosphate and calcium-fluoride nanocomposites with chlorhexidine. Dent Mater. 2012; 28: 573-583.

73. Khalichi P, Singh J, Cvitkovitch DG, Santerre JP. The influence of triethylene glycol derived from dental composite resins on the regulation of Streptococcus mutans gene expression. Biomaterials. 2009; 30: 452-459.

74. Sousa RP, Zanin IC, Lima JP, Vasconcelos SM, Melo MA, Beltrão HC, et al. In situ effects of restorative materials on dental biofilm and enamel demineralisation. J Dent. 2009; 37: 44-51.

75. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials-Fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater. 2007; 23: 343-362.

76. Nicholson JW, Aggarwal A, Czarnecka B, Limanowska-Shaw H. The rate of change of pH of lactic acid exposed to glass-ionomer dental cements. Biomaterials. 2000; 21: 1989-1993.

77. Nakajo K, Imazato S, Takahashi Y, Kiba W, Ebisu S, Takahashi N. Fluoride released from glass-ionomer cement is responsible to inhibit the acid production of caries-related oral streptococci. Dent Mater. 2009; 25: 703-708.

78. l-Naimi OT, Itota T, Hobson RS, McCabe JF. Fluoride release for restorative materials and its effect on biofilm formation in natural saliva. J Mater Sci Mater Med. 2008; 19: 1243-1248

Received : 21 Aug 2017
Accepted : 07 Sep 2017
Published : 10 Sep 2017
Journals
Annals of Otolaryngology and Rhinology
ISSN : 2379-948X
Launched : 2014
JSM Schizophrenia
Launched : 2016
Journal of Nausea
Launched : 2020
JSM Internal Medicine
Launched : 2016
JSM Hepatitis
Launched : 2016
JSM Oro Facial Surgeries
ISSN : 2578-3211
Launched : 2016
Journal of Human Nutrition and Food Science
ISSN : 2333-6706
Launched : 2013
JSM Regenerative Medicine and Bioengineering
ISSN : 2379-0490
Launched : 2013
JSM Spine
ISSN : 2578-3181
Launched : 2016
Archives of Palliative Care
ISSN : 2573-1165
Launched : 2016
JSM Nutritional Disorders
ISSN : 2578-3203
Launched : 2017
Annals of Neurodegenerative Disorders
ISSN : 2476-2032
Launched : 2016
Journal of Fever
ISSN : 2641-7782
Launched : 2017
JSM Bone Marrow Research
ISSN : 2578-3351
Launched : 2016
JSM Mathematics and Statistics
ISSN : 2578-3173
Launched : 2014
Journal of Autoimmunity and Research
ISSN : 2573-1173
Launched : 2014
JSM Arthritis
ISSN : 2475-9155
Launched : 2016
JSM Head and Neck Cancer-Cases and Reviews
ISSN : 2573-1610
Launched : 2016
JSM General Surgery Cases and Images
ISSN : 2573-1564
Launched : 2016
JSM Anatomy and Physiology
ISSN : 2573-1262
Launched : 2016
JSM Dental Surgery
ISSN : 2573-1548
Launched : 2016
Annals of Emergency Surgery
ISSN : 2573-1017
Launched : 2016
Annals of Mens Health and Wellness
ISSN : 2641-7707
Launched : 2017
Journal of Preventive Medicine and Health Care
ISSN : 2576-0084
Launched : 2018
Journal of Chronic Diseases and Management
ISSN : 2573-1300
Launched : 2016
Annals of Vaccines and Immunization
ISSN : 2378-9379
Launched : 2014
JSM Heart Surgery Cases and Images
ISSN : 2578-3157
Launched : 2016
Annals of Reproductive Medicine and Treatment
ISSN : 2573-1092
Launched : 2016
JSM Brain Science
ISSN : 2573-1289
Launched : 2016
JSM Biomarkers
ISSN : 2578-3815
Launched : 2014
JSM Biology
ISSN : 2475-9392
Launched : 2016
Archives of Stem Cell and Research
ISSN : 2578-3580
Launched : 2014
Annals of Clinical and Medical Microbiology
ISSN : 2578-3629
Launched : 2014
JSM Pediatric Surgery
ISSN : 2578-3149
Launched : 2017
Journal of Memory Disorder and Rehabilitation
ISSN : 2578-319X
Launched : 2016
JSM Tropical Medicine and Research
ISSN : 2578-3165
Launched : 2016
JSM Head and Face Medicine
ISSN : 2578-3793
Launched : 2016
JSM Cardiothoracic Surgery
ISSN : 2573-1297
Launched : 2016
JSM Bone and Joint Diseases
ISSN : 2578-3351
Launched : 2017
JSM Bioavailability and Bioequivalence
ISSN : 2641-7812
Launched : 2017
JSM Atherosclerosis
ISSN : 2573-1270
Launched : 2016
Journal of Genitourinary Disorders
ISSN : 2641-7790
Launched : 2017
Journal of Fractures and Sprains
ISSN : 2578-3831
Launched : 2016
Journal of Autism and Epilepsy
ISSN : 2641-7774
Launched : 2016
Annals of Marine Biology and Research
ISSN : 2573-105X
Launched : 2014
JSM Health Education & Primary Health Care
ISSN : 2578-3777
Launched : 2016
JSM Communication Disorders
ISSN : 2578-3807
Launched : 2016
Annals of Musculoskeletal Disorders
ISSN : 2578-3599
Launched : 2016
Annals of Virology and Research
ISSN : 2573-1122
Launched : 2014
JSM Renal Medicine
ISSN : 2573-1637
Launched : 2016
Journal of Muscle Health
ISSN : 2578-3823
Launched : 2016
JSM Genetics and Genomics
ISSN : 2334-1823
Launched : 2013
JSM Anxiety and Depression
ISSN : 2475-9139
Launched : 2016
Clinical Journal of Heart Diseases
ISSN : 2641-7766
Launched : 2016
Annals of Medicinal Chemistry and Research
ISSN : 2378-9336
Launched : 2014
JSM Pain and Management
ISSN : 2578-3378
Launched : 2016
JSM Women's Health
ISSN : 2578-3696
Launched : 2016
Clinical Research in HIV or AIDS
ISSN : 2374-0094
Launched : 2013
Journal of Endocrinology, Diabetes and Obesity
ISSN : 2333-6692
Launched : 2013
Journal of Substance Abuse and Alcoholism
ISSN : 2373-9363
Launched : 2013
JSM Neurosurgery and Spine
ISSN : 2373-9479
Launched : 2013
Journal of Liver and Clinical Research
ISSN : 2379-0830
Launched : 2014
Journal of Drug Design and Research
ISSN : 2379-089X
Launched : 2014
JSM Clinical Oncology and Research
ISSN : 2373-938X
Launched : 2013
JSM Bioinformatics, Genomics and Proteomics
ISSN : 2576-1102
Launched : 2014
JSM Chemistry
ISSN : 2334-1831
Launched : 2013
Journal of Trauma and Care
ISSN : 2573-1246
Launched : 2014
JSM Surgical Oncology and Research
ISSN : 2578-3688
Launched : 2016
Annals of Food Processing and Preservation
ISSN : 2573-1033
Launched : 2016
Journal of Radiology and Radiation Therapy
ISSN : 2333-7095
Launched : 2013
JSM Physical Medicine and Rehabilitation
ISSN : 2578-3572
Launched : 2016
Annals of Clinical Pathology
ISSN : 2373-9282
Launched : 2013
Annals of Cardiovascular Diseases
ISSN : 2641-7731
Launched : 2016
Journal of Behavior
ISSN : 2576-0076
Launched : 2016
Annals of Clinical and Experimental Metabolism
ISSN : 2572-2492
Launched : 2016
Clinical Research in Infectious Diseases
ISSN : 2379-0636
Launched : 2013
JSM Microbiology
ISSN : 2333-6455
Launched : 2013
Journal of Urology and Research
ISSN : 2379-951X
Launched : 2014
Journal of Family Medicine and Community Health
ISSN : 2379-0547
Launched : 2013
Annals of Pregnancy and Care
ISSN : 2578-336X
Launched : 2017
JSM Cell and Developmental Biology
ISSN : 2379-061X
Launched : 2013
Annals of Aquaculture and Research
ISSN : 2379-0881
Launched : 2014
Clinical Research in Pulmonology
ISSN : 2333-6625
Launched : 2013
Journal of Immunology and Clinical Research
ISSN : 2333-6714
Launched : 2013
Annals of Forensic Research and Analysis
ISSN : 2378-9476
Launched : 2014
JSM Biochemistry and Molecular Biology
ISSN : 2333-7109
Launched : 2013
Annals of Breast Cancer Research
ISSN : 2641-7685
Launched : 2016
Annals of Gerontology and Geriatric Research
ISSN : 2378-9409
Launched : 2014
Journal of Sleep Medicine and Disorders
ISSN : 2379-0822
Launched : 2014
JSM Burns and Trauma
ISSN : 2475-9406
Launched : 2016
Chemical Engineering and Process Techniques
ISSN : 2333-6633
Launched : 2013
Annals of Clinical Cytology and Pathology
ISSN : 2475-9430
Launched : 2014
JSM Allergy and Asthma
ISSN : 2573-1254
Launched : 2016
Journal of Neurological Disorders and Stroke
ISSN : 2334-2307
Launched : 2013
Annals of Sports Medicine and Research
ISSN : 2379-0571
Launched : 2014
JSM Sexual Medicine
ISSN : 2578-3718
Launched : 2016
Annals of Vascular Medicine and Research
ISSN : 2378-9344
Launched : 2014
JSM Biotechnology and Biomedical Engineering
ISSN : 2333-7117
Launched : 2013
Journal of Hematology and Transfusion
ISSN : 2333-6684
Launched : 2013
JSM Environmental Science and Ecology
ISSN : 2333-7141
Launched : 2013
Journal of Cardiology and Clinical Research
ISSN : 2333-6676
Launched : 2013
JSM Nanotechnology and Nanomedicine
ISSN : 2334-1815
Launched : 2013
Journal of Ear, Nose and Throat Disorders
ISSN : 2475-9473
Launched : 2016
JSM Ophthalmology
ISSN : 2333-6447
Launched : 2013
Journal of Pharmacology and Clinical Toxicology
ISSN : 2333-7079
Launched : 2013
Annals of Psychiatry and Mental Health
ISSN : 2374-0124
Launched : 2013
Medical Journal of Obstetrics and Gynecology
ISSN : 2333-6439
Launched : 2013
Annals of Pediatrics and Child Health
ISSN : 2373-9312
Launched : 2013
JSM Clinical Pharmaceutics
ISSN : 2379-9498
Launched : 2014
JSM Foot and Ankle
ISSN : 2475-9112
Launched : 2016
JSM Alzheimer's Disease and Related Dementia
ISSN : 2378-9565
Launched : 2014
Journal of Addiction Medicine and Therapy
ISSN : 2333-665X
Launched : 2013
Journal of Veterinary Medicine and Research
ISSN : 2378-931X
Launched : 2013
Annals of Public Health and Research
ISSN : 2378-9328
Launched : 2014
Annals of Orthopedics and Rheumatology
ISSN : 2373-9290
Launched : 2013
Journal of Clinical Nephrology and Research
ISSN : 2379-0652
Launched : 2014
Annals of Community Medicine and Practice
ISSN : 2475-9465
Launched : 2014
Annals of Biometrics and Biostatistics
ISSN : 2374-0116
Launched : 2013
JSM Clinical Case Reports
ISSN : 2373-9819
Launched : 2013
Journal of Cancer Biology and Research
ISSN : 2373-9436
Launched : 2013
Journal of Surgery and Transplantation Science
ISSN : 2379-0911
Launched : 2013
Journal of Dermatology and Clinical Research
ISSN : 2373-9371
Launched : 2013
JSM Gastroenterology and Hepatology
ISSN : 2373-9487
Launched : 2013
TEST Journal of Dentistry
ISSN : 1234-5678
Launched : 2014
Annals of Nursing and Practice
ISSN : 2379-9501
Launched : 2014
Author Information X