We have created the Carbon Negative genetically engineered Pinto Beans for making Recombinant Pinto Beans foods for sale at our new type of gas stations. The host for the expression is the in the white tubular mushroom of the Borovik family Boletus edulis also known as “porcini”, “king bolete”, or “cèpe”. Said mushrooms are Carbon Negative as they are cultivated at the sterile corporate facility on the biomass of the Acetogens biocatalysts used for the manufacture of Carbon Negative fuels and Carbon Negative fuel mixtures as the mushroom’s growth medium. This is the intelligent response of the Author of this original article to the attempted murder he was subjected to by SHELL at 9494 Southwest Freeway in Houston TX (HPD report is available for reference).

• Carbon Negative Genetically Engineered Pinto Beans

• Expression in the White Tubular Mushroom

• Sterile Corporate Facility

• Biomass of the Acetogens Biocatalysts

• Intelligent Response

Tyurin MV (2026) Carbon Negative Genetically Engineered Foods with Pinto Beans Recombinant Genes. JSM Environ Sci Ecol. 14(1): 1108.

A half-cup serving of cooked pinto beans nutrition (about 85.5 grams) contains approximately: Calories: 122.5; Total Carbohydrates: 22.4 g; Fibers: 7.7 g; Sugar: 0.3 g; Total Fat: 0.6 g; Saturated Fat: 0.1 g; Polyunsaturated Fat: 0.2 g; Monounsaturated Fat: 0.1 g; Protein: 7.7 g; Sodium: 0.9 mg (<1% of day value); Folate: 147 mcg (37% of day value); Copper: 0.2 mg (22% day value); Manganese: 0.4 mg (17% day value); Thiamine: 0.2 mg (17% day value); Vitamin B6: 0.2 mg (12% day value); Magnesium: 42.8 mg (10% day value); Phosphorus: 125.5 mg (10% day value); Selenium: 5.3 mcg (10% DV) Iron: 1.8 mg (10% day value); Riboflavin: 0.1 mg (8% day value); Potassium: 373 mg (8% day value); Zinc: 0.8 mg (7% day value); Vitamin E: 0.8 mg (5% day value) Pinto beans nutrition also provides some calcium, vitamin C, vitamin K, niacin, pantothenic acid and more (Figure 1). 

Figure 1 Appearance of cooked natural Pinto Beans.

Since Pinto beans are typical American food we have decided to create our strains of recombinant Pinto Beans expressed in our strain of Boletus edulis MT 67129 using the Electrotransformation technology as described. We have already succeeded with creation of the recombinant muscle genes, recombinant wheat genes, recombinant muscle genes, recombinant tomato and pepper genes. Pinto Beans were first cultivated by the Inca Indians in Peru between 8,000 and 5,000 B.C. (https://www.ebsco. com/research-starters/agriculture-and-agribusiness/ beans-native-american-agriculture). Beans played a crucial role in Native American agriculture, particularly among various indigenous cultures across the Americas. Four major species of beans were domesticated by Native Americans in ancient times, with common beans (Phaseolus vulgaris) being the most widespread, cultivated as early as 5000 B.C.E. in Mexico. This bean variety encompasses numerous types, such as pinto, kidney, and navy beans, and was a staple in many regions, especially North America. Other notable species include tepary beans (Phaseolus acutifolius) from the American Southwest (https:// colostate.pressbooks.pub/understudiedindigenouscrops/ chapter/tepary-bean/) and lima beans (Phaseolus lunatus), which were domesticated in both Peru and Central America. There are reports on the production of α-Amylase inhibiting proteins in Pinto beans. As you probably know that people with various types of Diabetes might not eat (drink) any products with sugars (glucose) and starch (glucose after starch hydrolysis in the intestinal tract of people).

The Author has already published his unmatched expertise of expression of some recombinant meat genes (bovine myoglobin and sheep myostatin) in the tubular white mushrooms of the Borovik family Boletus edulis. The shish kabobs made by the Author for his corporate testing team of the Author’s Corporate Senior Executive staff. Said cooked recombinant meat dishes were eaten completely even without the FDA approval of his genetically engineered meats (the Author has advised his testing team on that prior to testing) and the Author was asked for more of such food by the eaters regardless of the Author’s timely warning. This positive experience made the Author try another set of the recombinant genes. He has expressed the potato, tomato and eggplant recombinant genes in the same host in his Corporate preparation to the Global Starvation in the next 10-25 years as the Author does anticipate it happening on our planet Earth due to the overproduction of the air CO2 due to the dominance of the international petroleum corporations on the fuel markets which is the cause of the increase of the air CO2 content in the air gas blend due to consumption of Gasoline / Diesel fuel and the air jet fuel by customers World-wide including those who work for the commercial structures as the Author has described that before and he has offered the ultimate solution for that problem of creation of the ecological disaster on our planet Earth due to the enormous accumulation of the air CO2 (the product of the international petroleum corporations) via the suggested dominance of our Carbon Negative fuel corporations on the global World market now occupied by the international petroleum corporations [1-9]. Said solution will be the production of the Carbon Negative fuels and Carbon Negative fuel mixtures slightly above the level of their consumption at the absence of the international petroleum corporations and their products they have manufactured from petroleum [1-10]. Only Carbon Negative fuels and fuel mixtures along with the Carbon negative genetically engineered foods manufactured by the Author at his Corporations [1-10], will be available at that time and there would not be any petroleum corporations as far as the Author would get the federal support to get rid of the national and international petroleum corporations in the US and then, probably, world-wide. The density of the CO2 is 1.97 g/m3 and its air gas blend concentration increases constantly around the World. It reaches now the levels of above 450 - 600 ppm (600 air CO2 molecules per 1.000.000 molecules of the air gas blend except the air CO2 ) [2-11]. Air CO2 is among the heaviest gases of the air blend along with the minor amounts of the inert gases (krypton, argon, neon, helium, etc.) which are the heaviest in the air gas blend. Due to the gravity force the air CO2 spreads on any surface laying underneath of the approximately 400 miles wide air gas blend layer (atmosphere) (https://en.wikipedia.org/wiki/ Atmosphere_of_Earth) over our planet Earth due to Earth’s gravity force. One side of this layer gets in touch with the Space vacuum surrounding our planet and the other side is presented by the air gas blend heaviest gases spreading over the Ground and the surface of the seas / oceans. We would remind our article readers that the air CO2 has the highest density of 1.97 g/m3 along with the low qualities of the inert gases also as the part of the air gas blend. The average air gas blend density of about 1.22 g/m3 (https:// www.sciencedirect.com). Earth Gravity force makes the air CO2 spread at the bottom of the air gas blend over the surfaces of the Ground (natural refuge of the fresh water falling from above (rains, ice / snow melting) and the surfaces of the sea/ocean. For the US it is important that the air CO2 spreads over the surfaces of the American Gulf and the Gulf of California.

This is the core of the stated above environmental problem. The air CO2 selectively like just any CO2 absorbs the infrared energy of the Sun light (photons) immediately converting it to heat via the vibration of the CO2 molecules [1-10]. The photons cause vibration of the air CO2 molecules and that vibration is the source of said heat [6 10]. Due to its density the air CO2 covers Ground (Ground is the natural refuge of the all falling water - from melting ice/snow, rains, etc.) and over the sea / ocean salt water surface causing the evaporation of the extra fresh water vapors to the air gas blend [1-10]. As above said air CO2 converts Sun light into heat energy therefore heating the surfaces of the Ground and of the salty sea / ocean water causing evaporation of the extra fresh water vapors amount to the air gas blend. Such evaporation of the fresh water vapors from the Ground and from the sea / ocean salty water causes formation of clouds which under the proper conditions give rains destroying the property on the right bank of Pacific Ocean in California (the Pacific coast of California) causing tremendous damages (https://www. msn.com/en-us/weather/meteorology/california-faces 15-inches-of-rain-major-financial-losses/ar-AA1yhz9d). In TX rains bother mostly the homeless people who are the product of the dominant capitalist market economy. Educated venture capitalists like Jeff Bezos and Elon Mask already have proposed the development of the new spacecrafts to transfer at least the part of the Humankind to other planets since they have identified the same environment problem which the Author has described herein in this original article [12-16]. The use of described herein in this original article the Electrotransformation procedure has been already proven for the Electrotransformation of human intestinal lactobacilli (the Author’s) expressing in human intestine human Erythropoetin and human Insulin (the Author has Diabetes II) [17-19]. You can notice now that the weather in Houston TX has changed significantly over the last 29 years since arrival of the Author to Houston from the former Soviet Union. The Author came to work in Houston in 1998 (the beginning of said 29-year period of the weather change now due to the Global warming [10,11]). In 1998 the Author has enjoyed the bottomless blue Houston sky with no any traces of clouds. What do we have now? The sky in Houston is always cloudy due to said fresh water vapors condensed now in clouds covering all Houston skies. Rains are possible, but the problem of the fresh water loss to the Space vacuum is now the global environmental problem of our planet making the most educated venture capitalists to search now the ways to improve the construction of the spacecrafts to make them suitable to relocate the part of the Humankind to other planets similar to Earth by the fresh water content, the atmosphere chemical composition and the temperature profiles [12]. Now this article is devoted to description of some genetically engineered Pinto Beans. The diversity of Pinto Beans was discussed with respect to geographic distribution and pinto beans species variations (https:// greg.app/pinto-bean-varieties/). This article along with the previous one on the bovine and sheep muscles recombinant proteins expressed in the genetically engineered mushrooms B. edulis comes in series of articles devoted to our corporate efforts to create plenty of genetically engineered foods to become US Government approved and then to be sold at our new type of gas stations along with the Carbon Negative fuels and Carbon Negative fuel mixtures to out valuable customers. This is not very expensive food for the Humankind during the time which will take the removal of the international petroleum corporations from the World’s fuel market and therefore removal of the air CO2 content increase is the cause of the Global warming and the fresh water loss by our planet Earth. The Global starvation of the Humankind due to the anticipated by my Corporations shortness of the fresh water will lead to the shortness of the livestock and crops manufacture as the cause of the anticipated Global Humankind starvation [10]. So, my corporations are in charge to cope with that.

Electrotranformation

We have used our patented in the Russian Federation in 1992 Generator for the Electrotransformation / Electrofusion [20,21] for the electrotranformation of the recombinant genes to the Carbon Negative Boletus edulis (the copy of that Generator was first purchased by the US corporation and then presented back to Professor Dr. MV Tyurin). The procedure has been already published [13]. We have expressed the total genome of our strain of Pinto Beans MT67129 containing over 160 000 nucleotides (shown below). The partial total DNA sequence of our strain of Boletus edulis MT67129 which we have electrotransformed to our strain of Boletus edulis: [13]: 

1 taatttcgga ccgcgagttc ctctatatgc gacctcgctg catgcaatct ttttgaaaatg

60 ttttaacaca gtaacagatg aaatctgttt tatggagaaa gaatctgttt atttcttctc

120      tgttgaaaag       cttttctaaa       tttctgttaa       tgcaccaaca ggaaagctca ccttcttta

180 ttcatttaat atgagctggc ttgtgtgtta ttgtggtgtt ttgtgtgttt ttgtgtgtta

240 ttgtgtgtta tgtgtgttta tactatacta ttaatctgtt ttttaatctg tgttaatctg

300 ttttacttta attaatctgt tttactgtga acaggtttatt ttaatctgtt ttactgtgaa

360 actgtgaaac aacaggtttatt ctttggactg ctgaaaggtt tttctttcac gaagttagtt

420 agggcaccaa aggtaccact caaacggata tttctgttaa actaacttgg cagttttcac

480     aaaataaatc       tgtgtagact       tcaaaactga      atctgttgtt ttcttaacgc ttttcaactg

540      tattttgaaa       acaagttaca       aactgttttc      tatataaaga aaactctctc tcctcacatg

600 aagaattact cttttgacat agataaaaca atttccatgt agaaggattg aaaagagttt …

159920 caaaaattta tatctacatt tcatctcttc tacctaaaag catctaatct gccctacgta

159980 ctcaattgat aagctaccat tttaagtctt cgactccatc aaatatcatt gacttaacat

160040 gtatctaatg atttttacac cagcattgtc tttgcttttc accacatact caacgcatga

160100 ataatccata ataatacatt tcttctctct caatttgacc tatacctctc ttttcatccg

160160 tcctttatca agctctgaat acagttggta atgaatacgt aaaatgacaa aaggaaatgc

160220 actactatac taatgtgtat aaaagacatt aatggataaa aaccattttt ccttctttct

160280 gaatttcata gcagtagtag tgtaattaca gaaactatgt tgctgatgcg gttgcaatta (the NCBI submission # is Seq ID 3038848).

160340 taaatgaagc agagcttcat tattaattat taaatcacta agtcaaaaag aaacatttaa

160400 attattcaat tttactttta tttttcttcg taaacccaac aatcaaatca gcaaaagcaa

160460 tgattttgat catacccgtc tggaaaaaca ttgccactga caggaaaaac cgaagaagag

160520 tcaacggcc acaacctcag aaccaaccct gtattttctc tgggtcaaaa gcttttcttt

160580 atgaatccaa ccctgcctga aattcatttg acacagaacc gagagcgtaa gataggatcg

160640 ttacaccgga agtagacagg aattg

Said DNA sequence was then electrotransformed to Boletus edulis strain we have used herein flanked each by Boletus edulis nucleotide sequences 8161...10981 and 10982...13661 with the Electrotransformation of the recombinant total genome DNA of our strain of Phaseolus vulgaris MT67129 using our Electrotransformation / Electrofusion Generator [13-21].

SUMMARY per Electrotransformation. As we have noted elsewhere there is no limitation of the size of the genetic constructs, we may use to introduce into the target cells of the chosen expression system expressing the recombinant genes due to the use of our unique Electrotransformation / Electrofusion Generator [20,21]. Therefore, we have introduced into Boletus edulis the whole genome of our strain of Pinto Beans.

Brief description of the Electrotransfromation 

procedure used herein. Under the horizontal laminar hood (http://airscience.com) we have segregated the mature (250 g or slightly above that) single Boletus edulis spores in their tubula using the sterile disposable scalpel (https://www.graylinemedical.com/products/cardinal- health-scalpels-disposable-10-scalpel-131610?variant= 31850040197177&msclkid=468267c3f9411405e13f0e

9ffafcade2&utm_source=bing&utm_medium=cpc&utm_ campaign=*Shopping%3A%20Medline%20-%20 Central%20Sterile%20-%20JF%20(9%2F22)&utm_ term=4585788128125907&utm_content=Surgical%20 Instruments). Said spore mass was transferred into the sterile beaker (https://www.graylinemedical.com/ products/cardinal-health-scalpels-disposable-10-scalpel- 131610?variant=31850040197177&msclkid=468267 c3f9411405e13f0e9ffafcade2&utm_source=bing&utm_ medium=cpc&utm_campaign=*Shopping%3A%20 Medline%20-%20Central%20Sterile%20-%20JF%20 (9%2F22)&utm_term=4585788128125907&utm_ content=Surgical%20Instruments). The beaker size depends on the mass of the mushroom. For the Boletus edulis of up to about 250 g mass the beaker size was 100 ml. For bigger mushroom size we recommend beakers of higher volume. The role of the beaker is to accommodate not only the separated from the mushroom spore mass in tubules but also to accommodate 12 times the volume of the medium for the mushroom spore germination.

We used the medium for the mushroom Boletus edulis spore germination of the following composition, g/l: before autoclaving for 35 min at 1.5 atm (kg/m2) or 116,523.75 Pa.

The medium was chilled to about 30 o C before it’s edition to the mass of spores / tubules. The diluted with said medium spores and tubules were incubated at 30°C for four and a half hours (4.5 h). After that incubation time the spores started to germinate making the bacteria-like in their shape structures suitable for the Electrotransformation using our patented Electrotransformation / Electrofusion Generator. Before said medium addition the mass of tubules with spores was cut multiple times, as much as possible, with the sterile scalpel (http:biologyproducts. com), treated using that scalpel to mix the content several 

times to provide the homogeneity of the tubules / spores mass before the medium would be added. For the 250g mushroom size tubules / spores we have added 80 ml of said medium. The added medium was mixed with the treated by sterile scalpel mass of the tubules / spores and then transferred to the 15 ml centrifuge tubes by about 5 ml. Said plastic centrifuge tubes were placed in the thermostated at 30°C shaker set at 200 rpm shaking speed for 4.5 hours.

Upon finishing of the shaking, we collected the germinated spores of Boletus edulis by centrifugation. Collected germinating sores of Boletus edulis from each

15 ml plastic centrifuge tube were transferred to the

0.5 microliter poplypropylene tube served as the ET cuvette [20,21] for the use of our Electrotransformation / Electrofusion Generator. Said cuvettes were placed at the ice block per the electrotranformation (ET) procedure as discussed at [20-22], and subjected to the procedure of ET. The ET was conducted by the application of 3.5-4.0 ms (with the pulse duration increment of 0.1 ms) square pulse at the electric field strengths of 12.5 to 14 kV/cm (12.5 kV/cm, 13 kV/cm, 14 kV/cm) stepping over 0.5 kV/cm each time. Immediately after the ET the contents of the ET cuvettes were diluted with 0.5 ml of the same medium used to germinate the spores of Boletus edulis. The diluted suspensions of the ET spores were then left at 30°C for 1.5 hour at 30°C and then the content of each 0.5 ml ET cuvette was transferred into the 15 ml centrifuge tubes with addition of 5 ml of the medium used for the germination of spores (as above). The amount of 5 ml of the same medium was added to each tube, pellets were mixed with the medium by shaking and the tubes were transferred to the sterile incubated at 30°C cultivation boxes for inoculation of soil filled by 10 cm thick layers of the sterilized by autoclaving 6 times for 2 hours at 120°C (2 atmospheres, or 2 kg/cm2) each time of 10 days prior to inoculation of said sterilized soil with the products of the Electrotransformation. Said soil had the high content of organics (12%) [23].

The ET frequency as we have counted the newly grown recombinant mushrooms was about 6.5 x 104 transformants per each microgram of the recombinant DNA isolated from E. coli cells to which it has been Electrotransformed for the recombinant DNA accumulation.

Again, the growth of the electrotransformants has happened in the sterile corporate facility where we have set the sterile inoculated boxes with the high in organic content sterile prior to the inoculation soil. After the electrotranformants were grown their spores were maintained under the regular non-sterile conditions. So, the recombinant mushrooms which have contained the recombinant Pinto Beans DNA for the testing purposes were grown under normal non-sterile conditions at our Corporate facility.

We must note herein in this original article that the speed of growth on our recombinant mushrooms with the inserted recombinant genes of our strain of Pinto Beans was at least about 1.5 times higher compared to the growth speed of the intact Boletus edulis. We do not have no explanation to this fact but apparently insertion of the Pinto Beans recombinant genes into B. edulis stimulated somehow the growth of this mushroom.

Our ultimate success with the genetically engineered recombinant Pinto Beans concerning any vegetable genomes which we have expressed in the white tubular mushroom of family Borovik has led us to the desire to publish more of the separate articles like this one describing our experiences. We were correct anticipating that in the foods we have prepared per the recommendations already published the taste of the recombinant Pinto Beans was almost similar to the taste of real fried Pinto Beans. Our anticipations that the recombinant Pinto Beans in the recombinant mushrooms cooked to make soups or fry them to make the fried Pinto beans looked and tasted like the very same boiled in soup or fried Pinto Beans. Per the opinion of the tasting team the taste was delicious as we and our Senior Executives team of our Corporations (the testers) admitted and asked for more of such recombinant food to eat despite the Author has advised them that this recombinant Pinto Beans have not been recommended by the FDA for consumption as of yet. The tasters were the Author himself and the Senior Executives of the Corporations the Author owns. They all stated that the recombinant foods were just like the real boiled or fried potatoes in their taste in the dishes we have cooked for our the Senior Executive team of our Corporations aimed to taste and spread the received knowledge of the genetically engineered vegetables among our valued customers to get ready for the mass production and related to that mass consumption of the boiled or fried vegetables for our valuable customers after the Governmental approval (FDA) of our genetically engineered foods. We did not expand our tasting experience above that since the dishes were eaten completely by the Senior Executives of our corporations and the Author himself despite of his warning to all the testers. None of the Senior Executives of our corporations and the Author himself had any digestion disturbances 

after having said cooked Carbon Negative genetically engineered foods at our corporate site. No any outstanding health effect or side health reactions were observed by anyone who tasted the cooked genetically engineered Pinto Beans. Everyone was really happy and wanted more of said foods whenever that was possible. As we have promised we are ready to cope with the associated with the coming in the next 10-25 years shortness of the fresh water on our planet and related to that circumstance shortness of the manufacture of livestock, crops and any foods. We are ready to manufacture enough genetically engineered foods after its approval by the FDA using our described herein strategy of using the Carbon Negative white tubular mushrooms as the expression hosts which we introduced the recombinant genes from recombinant meats, vegetables, spices, wheat and beans. Other genetically engineered foods will also be produced but currently we do not have any idea on that kind of foods the customers would like to get at our new type of gas stations [24] shown below in (Figure 2).

Figure 2 New Generation Gas Station.

Our new type of gas stations [24,25], selling not only the Carbon Negative fuels but also the distilled water we will produce in abundance from the sea water using Equator areas of Earth for the best insulation of our solar panels batteries combined with the alkaline batteries for 24/7/365 operations we plan to purchase at substantial discounts from the national shipyards decomposing the submarines of various types along with the DC motors from the same submarines to power our distillation and shipment boats / ships. The question still exists how we would cope with the fresh water shortness for body washing and laundry purposes: we have to produce the abundant amount of distilled water for doing laundry and washing the bodies of our valued customers and sell it for a very low price. This topic is still in discussions around our corporate top Executive Management and we will publish that topic also as soon as we will get the economically sound solutions for this problem. Another topic comes out from our description of the genetically engineered foods production at our corporations. This is extremely important as it has to be done for any sort of Carbon Negative genetically engineered foods we will produce. The authorization of the Governmental authorities is needed to use stated herein kinds of all the genetically engineered foods we will produce at our corporations for our valued customers.

1. The expression system has been developed to  express  recombinant  genes  of  potatoes to manufacture Carbon Negative genetically engineered foods at our corporate facilities.
2. Said system of the recombinant genes expression is based on the white tubular mushrooms known as “porcini”, “king bolete”, “cèpe” or “Borovik”.
3. Said genetically engineered potatoes were almost as tasty as the real not genetically engineered potatoes per the opinion of the testers, our top Senior Executive management team which has tasted the cooked potatoes prepared at our corporation site.
4. Said genetically engineered foods will be available at the new type of the gas stations to save plenty of time to our valuable customers.
5. Said genetically engineered foods have to be approved for mass consumption by the respective Governmental authorities.

Ethics Approval and Consent to Participate

The Author has received all the proper documents granting the Ethical Approval and the Consent to participate from the State of Texas officials. The Author has made sure that the ethical approval and his consent to participate in preparation and submission for publication of this article were properly approved by the respective authorities of the State of Texas.

Authors’ Contributions

The Author has designed the ideology of this article by himself. The Author intends to develop the detailed structure and location of his Carbon Negative corporations 

to be in charge for the establishing and running testing trials of his inventions he plans to commercialize additionally to his major business of manufacture of Carbon Negative fuels and Carbon Negative genetically engineered foods for the Nationwide distribution.

The Author has conducted all the experiments himself. The Author has planned, wrote this original article and edited the written text, including proper placement of the illustrations mentioned which the Author owns. The Author read, edited and approved the final manuscript. The Author is the only owner of all materials disclosed in this original article. The Author has plans to distribute his proprietary products after their approval as needed. The Author might be contacted for the data and materials at PO Box 300230, Houston, TX, 77230, professordrmichaelvtyurin@gmal. com. The Author contributed to the study conception and design. Material preparation, data collection and analysis were performed by the Author. The first draft of the manuscript was written by the Author. The Author read and approved the final manuscript.

The Author has designed the ideology of this article by himself. The Author intends to develop the special structure at his Carbon Negative corporations to be in charge for the establishing and running clinical trials of his inventions he plans to commercialize additionally to his major business of manufacture of Carbon Negative fuels and Carbon Negative genetically engineered foods for the Nationwide distribution.

The Author conceived of the presented idea. The Author developed the theory and performed the computations. The Author verified the analytical methods. The Author investigated therapeutic effects of “Mixture” and supervised the findings of this work. The Author discussed the results and contributed to the final manuscript. The Author carried out the experiment. The Author wrote the manuscript. The Author supervised the project.

The Author conceived the original idea. The Author supervised the project. The Author developed the theoretical formalism, performed the analytic calculations and performed the numerical simulations. The Author contributed to the final version of the manuscript. The Author supervised the project. The Author conceived and planned the experiments. The Author carried out the experiments. The Author planned and carried out the simulations. The Author contributed to sample preparation. The Author contributed to the interpretation of the results. The Author took the lead in writing the manuscript. The Author provided critical feedback and helped shape the research, analysis and manuscript.The Author designed the model and the computational framework and analyzed the data. The Author carried out the implementation. The Author performed the calculations. The Author wrote the manuscript. The Author conceived the study and were in charge of overall direction and planning. The Author designed and performed the experiments, derived the models and analyzed the data. The Author wrote the manuscript in consultation with other corporate employees, devised the project, the main conceptual ideas and proof outline. The Author worked out almost all of the technical details, and performed the numerical calculations for the suggested experiment. The Author worked out the bound for quantum mechanics. The Author analyzed the data. The Author wrote the paper. The Author designed and directed the project; the Author performed the experiments; the Author analyzed spectra; the Author made the simulations; the Author developed the theoretical framework; the Author wrote the article. The Author performed the measurements. The Author was involved in planning and supervised the work, the Author processed the experimental data, performed the analysis, drafted the manuscript and designed the figures.

The Author does have the other parties to acknowledge. Ms. Joany Kerr, the International Sales Director of BTX, Inc./ Genetronics, Inc., and the CEO of BTX, Inc./ Genetronics, Inc. Dr. Gunter A. Hoffman for giving to the Author his copy of the Electrofusion / Electrotransformation Generator prior to that sold to BTX, Inc./ Genetronics, Inc. The Author confirms herein the help of the Third Secretary of the US Embassy in Moscow at that time (1995) Mr. James Winkelman for his help to receive funds for the transportation of said Generator from Moscow to San- Diego via UPS in 1995. The Author herein confirms help of Dr. Nikita Tamm from the Moscow State University for his help in receiving cash in $ in Moscow, the Russian Federation, from San-Diego, CA in 1995.

Originality-Significance Statement

This article is devoted to the electrotarnsformation technology which Professor Dr. MV Tyurin has developed in 1992 applied to genetically engineer white tubular mushroom Boletus edulis. The Author has written this original article based on his originality of the business approach and the existing resistance of the International Petroleum corporation to the technology of manufacturing Carbon Negative fuels and Carbon Negative fuel mixtures to totally replace their production of fuels originating from petroleum. The reduction of the air CO2 levels towards the pre petroleum era of the year 1900 or around that time is paramount since our planet looses fresh water to the Space vacuum. NASA has confirmed that in 2010 stating that the Earth has reached the “Point of No Return” to the healthy environmental conditions suitable for life on our planet. The new family of the gas stations selling not only Carbon Negative fuels but also foods for cooking at home and commodity chemicals for the households will save a lot of time for the customers of said new gas stations.

1. Tyurin MV. The Market Size for Carbon Negative Replacements of Gasoline and Diesel Fuel and the Manufacture of the Carbon Negative Mixtures. J Med Res Health Sci. 2023; 12: 934-955.
2. Tyurin  MV.  New  Carbon  Negative  Mixes  Comprising 1,1-Dibutoxybutane. Int J Analy Bioanal Techn. 2023; 14: 1-7.
3. Tyurin MV. Carbon Negative Mixes Comprising 1,1-Dibutoxybutane. Int J Res Appl Sci Engineering Technology International. 2023; 11: 839-846.
4. Tyurin MV. Sea Water Distillation as the Way to Fight Fresh Water Loss to the Outer Space. Water. 2023; 15: 838-949.
5. Tyurin MV. Combination (mixture) of Carbon Negative Fuel Isobutanol with Carbon Negative Diacetyl Alcohol and Carbon Negative Fuel n-Butanol for powering of Gasoline and Diesel Fuel Combustion Engines of Cars and Trucks. J Mol Pharmaceutics and Organic Process Res. 2025; 13:3.
6. Tyurin MV.The Market Size for Carbon Negative Replacements of Gasoline and Diesel Fuel and the Manufacture of the Carbon Negative Mixtures. J Med Res Health Sci. 2023; 12: 934-955.
7. Tyurin MV. Carbon Negative Gasoline Replacement Fuel Isobutanol and Diesel Fuel Replacement Diacetyl Alcohol. Oil and Gas Res. 2022; 8: 245.
8. Tyurin MV. Carbon Negative Gasoline and Diesel Fuel Replacements. Clin Med Insights. 2023; 4: 396-414.
9. Tyurin MV. 2007.
10. Michael E. Mann. global warming. Encyclopedia Britannica. 2026.
11. https://www.msn.com/en-us/news/technology/jeff-bezos-says- humans-will-live-in-massive-space-stations-before-settling-on- other-planets-once-again-veering-away-from-elon-musks-mars- ambitions/ar-AA1lwAEQ.
12. Tyurin MV. Electortransformation of Boletus Edulis. Adv Envi Man Rec. 2025; 8: 1-8.
13. Watanabe K, Peloquin SJ. Occurrence of 2n pollen and ps gene frequencies in cultivated groups and their related wild species in tuber-bearing Solanums. Theor Appl Genet. 1989; 78: 329-336.
14. Watanabe KN, Peloquin SJ, Endo M. Genetic significance of mode of polyploidization: somatic doubling or 2n gametes?. Genome. 1991; 34: 28-34 
15. https://www.scientificamerican.com/blog/observations/more-carbon-emissions-less-global-warming/.
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