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High dose Folic Acid Plus Vitamins B6 and B12 Added to Adalimumab can Worsen Psoriasis In Patients with Low BMIs and in Others May Cause Unanticipated EKG Effects

Short Communication | Open Access | Volume 3 | Issue 1

  • 1. Department of Dermatology, Wayne State University, USA
  • 2. Dermatology Section, John d. Dingell VA Medical Center, USA
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Corresponding Authors
Peter J. Aronson, MD, Dermatology, John D. Dingell VA Medical Center, 11M-DERM, 4646 John R, Detroit, MI 4820,USA
Abstract

This was to be a study of 16 weeks adalimumab then 12 weeks adalimumab plus 5mg. folic acid, B6 and B12 in 10 patients. In the adalimumub-vitamin study four subjects showed psoriasis area and severity index reduction after B vitamin addition by week 28 (one more reported clearing by week 38). The study was stopped after eight subjects because one developed pneumonia before vitamins and, after vitamins were added, one subject who improved developed left axis deviation on vitamins. Two subjects flared. They had the 2 lowest BMIs.

Both were female. Both had baseline vascular endothelial growth factor (VEGF) levels of 140 pg/ml or higher. One male subject did also. One female had elevated H. pylori antibodies.

Five mg folic acid daily promotes creation of anti-inflammatory dimeric endothelial nitric oxide synthase (eNOS) over the inflammatory monomeric eNOSassuming folic acid absorption is not blocked by Helicobacter pylori. Leptin can maintain eNOS function. Lighter patients treated thusly would have less stable pro and anti-eNOS actions withproinflammatoryVEGF less opposed.

Keywords
  • B vitamins
  • Homocysteine
  • eNOS
  • iNOS
  • VEGF
Citation

Aronson PJ, Swearingen J, Malick F, Mehregan DA, Mehregan DR (2015) High dose Folic Acid Plus Vitamins B6 and B12 Added to Adalim- umab can Worsen Psoriasis In Patients with Low BMIs and in Others May Cause Unanticipated EKG Effects. J Dermatolog Clin Res 3(1): 1039.

ABBREVIATIONS

BMI: body mass index; eNOS: endothelial nitric oxide synthase; pg/ml: picograms per milliliter; iNOS: inducible nitric oxide synthase; IV: intravenous; mcg: micrograms; mg: milligrams; mRNA: mitochondrial ribonucleic acid; NO: nitric oxide; PASI: psoriasis area and severity index; PPD: purified protein derivative tuberculosis skin test; TB: tuberculosis; VEGF: vascular endothelial growth factor; VEGFR-2: vascular endothelial growth factor receptor 2

INTRODUCTION

Homocysteine affects psoriasis by affecting neutrophil function [1,2]. Folic acid with vitamins B6 and B12 lowers homocysteine [3]. Plasma folic acid levels inversely correlate with psoriasis area and severity index (PASI). [4]

Folic acid derivatives reduce oxidative injury by creating anti- inflammatory endothelial NOS (eNOS) dimers [5-6] A published study suggested increased psoriasis risk with low dietary folate equivalent in beer drinkers, however, study median daily folate intake was 976-1000 mcg [7].

Flow –mediated vasodilation is regulated by endothelial nitric oxide (NO) release. Endothelial nitric oxide synthase (eNOS) synthesizes NO. Five mg/day, but not 400 mcg/day, folic acid improves blood vessel cell function measured by flow mediated vasodilation [8]. Low dose folic acid creates uncoupled monomeric eNOS results leading to reduction of net NO synthesis and to pro-inflammatory superoxide generation a change called eNOS uncoupling [9,10]. High dose folates in contrast are anti- inflammatory [9].

Endothelial NOS generally produces low NO levels. Inducible NOS (iNOS) is induced by cytokines and microbial factors and produces high NO levels [1,12]. mRNA expression of iNOS appears to promote inflammation since it is markedly and continuously increased in psoriasis [12]. However, iNOS coupling using tetrahydrobiopterin and L-arginine is anti-inflammatory and there is an anti-inflammatory effect of iNOS-created NO after exposure to reactive oxygen species [1,12]. Quantitative statistical analysis of two genome wide association studies of psoriasis identified iNOS as one of several susceptibility loci for psoriasis [13]. Etanercept (another TNF-alpha blocking medication)-cleared psoriasis skin has 87% less iNOS [14]. High dose folic acid reduces eNOS [15] and, at least in animal models, iNOS [16].

Two published cases by the authors showed improved of psoriasis at least in part by the addition of 5 mg folic acid, 100 mg vitamin B6 and 100 mcg B12 [17].

MATERIALS AND METHODS

Subjects were non-immunocompromised and ≥18 years old with moderate-to-severe (>10% BSA) plaque psoriasis who are candidates for systemic therapy and adalimumab naive.

There was to be a total of 10 subjects.

Subjects must have met pre-study inclusion and exclusion criteria (Tables 1, 2).

Table 1: Adalimumab-B-vitamin Study Inclusion Criteria.

Subject

Is a non-immunocompromised male or female\ 18 years of age or older

Has moderate-to-severe (≥10% total body surface area prior to

therapy) plaque psoriasis

Has a negative urine pregnancy test within 7 days before the first dose of adalimumab in all women (except those surgically sterile or at least 5 years postmenopausal)

Must sign/date the appropriate written informed consent and HIPAA authorization

If sexually active: subjects of childbearing potential must agree to use medically acceptable form of contraception during screening and throughout the study

Has no evidence of active or latent tuberculosis based on a negative PPD skin test performed at screening, or within one year of starting this study. Patients with documentation of adequately treated tuberculosis may be enrolled. Patients who are PPD positive and CXR negative can be enrolled if they finish appropriate INH prophylaxis prior to enrollment

Is willing and able to self-administer subcutaneous injections or to have a qualified person available to administer subcutaneous injections

Agrees to comply with protocol requirements, attend all regularly study visits and is considered to be a good study subject

Meets concomitant medication washout requirements

Is willing to use only allowed psoriasis medications and treatments (see below entitled “Allowed psoriasis medications/treatment”) and agree not to start any topical, systemic, or phototherapy for psoriasis during the study period

Is adalimumab naïve.

Table 2: Adalimumab B-Vitamin Study Exclusion Criteria.

Subject

Has erythrodermic, pustular, or guttate psoriasis

Has evidence of skin conditions other than psoriasis that would interfere with study-related evaluations of psoriasis

Has a known sensitivity to any component of the study medications

Has evidence of active infections such as fevers, chills, sweats, or history of untreated

Has had Lyme disease and active severe infections within 4 weeks before screening visit, or between the screening and Week 0 visits.

Has a history of listeriosis, untreated TB, persistent or active infections requiring hospitalization or treatment with IV antibiotics, IV antiretrovirals, or IV antifungals within 30

Days of baseline, OR oral antibiotics, antivirals, or antifungals for purpose of treating infection,

Within 14 days of baseline

Has a positive PPD and positive chest x-ray for latent or active tuberculosis

Has positive PPD and negative chest x-ray that have not completed appropriate INH prophylaxis

Has history of immune compromised status [e.g. human immunodeficiency virus (HIV) positive status or other immune suppressing drug] or a congenital or acquired immunodeficiency

Has a poorly controlled medical condition including, but not limited to, unstable cardiovascular disease, poorly controlled diabetes, recent stroke, history of recurrent infections, or any other condition for which, in the opinion of the investigator, participation in the study would place the subject at risk

Has a history of congestive heart failure Has a history of demyelinating CNS disease

Has a history of malignancy (other than previously treated localized carcinoma in situ of the                    cervix or previously treated non- melanoma skin cancer)

Has a history of or ongoing drug or alcohol abuse

Has past or present psychiatric morbidity which may compromise the study in the opinion of the investigator

Is a pregnant woman, nursing mother, or woman planning to become pregnant during the study or within 150 days after the last dose of study medication, or male subjects planning a pregnancy with their spouse/partner while in the study are to be excluded

Plans to receive any live vaccines during the study Has a history of liver disease (e.g. hepatitis, cirrhosis)

Is currently enrolled in another clinical study and/or is on treatment with another experimental drug or approved therapy for experimental use within 30 days prior to Week 0

Has previously been enrolled in this study

Cannot commit to all the assessments required by the protocol

Has any disorder that compromises the ability of the subject to give written informed consent and/or to comply with study procedures

Is considered by the investigator, for any reason, to be an unsuitable candidate for study participation

Cannot or does not wish to comply with the protocol washout requirements

Is on oral folic acid in doses greater than the minimal daily requirements

Is on doses of vitamins greater than the minimal daily requirements (multivitamins are allowed)

Has history of colon polyps or colon cancer Has a prior history of adalimumab therapy.

They must undergo an active washout (discontinue restricted medications) prior to starting the study treatment at Week 0

Adalimumab was given   by subcutaneous injection   Week 0- 80 mg then Week 1- 40 mg, then 40 mg every other week to completer a 28 weeks study. At week 16 folic acid 5mg, vitamin B6 100 mg and B12 1000 mcg were added daily. All were to be measured at: Week 0 [Baseline], and Week 28.

Height and Weight, PASI scores, Physician Global Assessment of Psoriasis, Static Physician Global Assessment of Psoriasis and Dermatology Life Quality Index, serum magnesium, serum phosphorus, complete blood cell count with differential, homocysteine, serum B6, serum B12, serum folic acid, and VEGF measured at weeks 0, 16 and 28. EKG was to be measured weeks 0, 16 and 28. Helicobacter pylori antibody was to be measured at baseline.

Post drug portion of study telephone call: a telephone callday 70 post study.

Table 3 summarizes the results (but after the study was approved, it was found that full adalimumab effect may not occur until week 33) [18].

Table 3: Adalimumab- B Vitamin Study Results.

 

Subject

 

Sex

 

Age

 

Week

Weight In Pounds

BMI

week 0

 

PASI

 

DLQI

 

Folic

 

H. pylori

 

Hcy

 

VEGF

 

EKG

7

F

23

0

136

23

13.6

16

18.3

 

7.3

259

normal

 

 

 

4

136.6

 

0.4

3

 

 

 

 

 

 

 

 

16

134.2

 

0.4

3

18.2

<0.89

6.5

39

normal

 

 

 

28

134.4

 

0.8

2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

8

F

65

0

163.6

24.9

8

17

6.4

5.7

24.7

147

normal

 

 

 

4

159.4

 

3.2

8

 

 

 

 

 

 

 

 

16

169

 

2.6

9

4.3

 

15.7

68

n/s ST-T

 

 

 

28

165.8

 

3.4

9

 

 

 

 

n/s ST-T

 

 

 

 

 

 

 

 

 

 

 

 

 

3

M

31

0

202

26.6

12.2

10

7.3

0.39

8.6

144

normal

 

 

 

4

202.8

 

1.5

2

 

 

 

 

 

 

 

 

16

202.4

 

1.4

0

5.5

 

8.1

27

normal

 

 

 

28

206

 

0.5

1

11.4

0.47

5

37

LAD

 

 

 

 

 

 

 

 

 

 

 

 

 

10

M

48

0

193.2

27.3

30

11

11.2

0.55

16.8

44

normal

 

 

 

4

195.8

 

11

0

 

 

 

 

 

 

 

 

16

201.6

 

0.6

0

11.7

 

 

19

normal

 

 

 

28

195.8

 

0.6

0

>20

0.52

12.7

28

normal

 

 

 

 

 

 

 

 

 

 

 

 

 

9

M

56

0

171

29.4

15.3

23

9

 

12.9

19

normal

 

 

 

2

168

 

6.3

5

 

 

 

 

 

 

 

 

16

159.8

 

0.5

0

6.9

 

12.5

26

LAD

 

 

 

28

158.8

 

0

0

>20

5.07

18

126

normal

 

 

 

 

 

 

 

 

 

 

 

 

 

2

M

44

0

225

31.4

35.6

11

 

 

 

 

 

 

 

 

4

226

 

18.2

2

 

 

 

 

 

 

 

 

16

228

 

2.8

4

>20

 

11

35

brady

 

 

 

28

218.8

 

1.5

0

>20

0.22

9.8

22

brady

 

 

 

 

 

 

 

 

 

 

 

 

 

11

M

62

0

241.9

37.9

36

30

13.3

<0.89

15

68

RBBB

 

 

 

4

242

 

15.2

5

 

 

 

 

 

 

 

 

16

234.5

 

4.4

2

>20

 

18

55

RBBB

 

 

 

28

233.6

 

3

0

>20

 

13.4

222

RBBB

 

 

 

 

 

 

 

 

 

 

 

 

 

1

F

49

0

255.2

44.8

25.9

15

7.4

0.27

11.9

 

Prolonged QT

 

 

 

4

 

 

30.9

3

 

 

 

 

 

 

 

 

SAE

 

 

 

 

 

 

 

 

 

Note: Subjects 4, 5 and 6 failed screening

Abbreviations: BMI: body mass index; brady: sinus bradycardia; DLQI: Dermatology Life Quality Index; EKG: electrocardiogram; F:Female ; Hcy: serum homocysteine in micromoles per liter; H. Pylori: Helicobacter pylori IgG enzyme linked immunosorbent assay; M:male; n/s ST-T: nonspecific ST segment and T wave electrocardiogram changes; PASI: Physician’s Area and Severity Index; RBBB: right bundle branch block; SAE: serious adverse event; VEGF: vascular endothelial growth factor in pictograms per milliliter.

 

RESULTS AND DISCUSSION

The study was stopped after 8 subjects were enrolled with only 7 completing the study because one subject was hospitalized for pneumonia before starting the vitamins and was therefore 

disqualified from completing the study this was a serious adverse event. second subject developed left axis deviation at week 28 after vitamins were added to adalimumab. One subject developed st-t wave EKG changes not thought to be significantly different from a baseline EKG which was read as normal. A third subject, however, normalized left axis deviation after B vitamins were added. Two subjects’ psoriasis worsened after the vitamins were added to the adalimumab. These 2 subjects had the 2 lowest BMIs. Both are women, one was premenopausal and the other post-menopausal. Both had baseline vascular endothelial growth factor levels (VEGF) greater or equal to 140 pg/ml (a third subject with levels this high improved). Helicobacter pylori antibody was elevated prior to B vitamins in one subject who flared and one who improved (Table 3).

VEGF fell after just adalimumab was added in 4 of 5 subjects where it was measured but after vitamins were added levels 4 of 5 subjects’ VEGF rose including 3 of the 4 subjects who improved and one subject whose PASI did not change after the vitamins were added (Table 3) (though he reported clearing when contacted day 70 after the week 28 study visit). Homocysteine levels fell in 4 subjects who improved or did not worsen and rose in 1 after B vitamins were added (Table 3). One subject whose homocysteine (and all 3 B vitamin levels) rose also had his VEGF rise after vitamins. He was the subject whose left axis deviation left after B vitamins were added to his adalimumab (Table 3).

Psoriasis worsened in the two subjects with the lowest BMI. It has been shown that VEGF levels Therefore it was unfortunate that leptin was not measured in this study. Endothelial nitric oxide synthase (eNOS) is maintained when blood vessels are incubated with leptin. [19] Patients with psoriasis have increased leptin. [20] Lower BMI patients generally have less leptin [21]. TNF alpha reduces eNOS mRNA [22]. Adalimumab, by inhibiting TNF-alpha, could increase eNOS mRNA. High folic acid promotes anti-inflammatory dimeric eNOS. Therefore, it seems that obese people would have better maintained dimeric eNOS activity than thinner people. However, on lower folic acid doses the obese have more and better maintained proinflammatory monomeric eNOS.

Female sex cannot be eliminated as relevant to a subject flaring after vitamins are added to adalimumab. Both subjects who flared were women, one pre- and the other post-menopausal, We, however have published an erythrodermic psoriasis patient who responded well to 5 mg folic acid plus B12 and B6 [19]. A study has shown that serum leptin levels in male and female psoriasis patients are similar [21]. Others have found no differences in VEGF blood concentrations in men and women [23]. In our study, however, baseline VEGF was above or equal to 140 pg/ml in both women while such baselines levels were found in only one of four men so studied.

Elevated Helicobacter pylori IgG is not 100% predictive of H. pylori infection [24]. If present it may inhibit folic acid absorption raising homocysteine [25] or, when high doses are given, creating pro-inflammatory monomeric eNOS instead of anti-inflammatory dimeric eNOS [9-12]. Both situations can cause psoriasis to flare.

Psoriasis is a disorder with elevated VEGF [26]. Adalimumab lowers VEGF [27]. The addition of B vitamins may increase VEGF flaring psoriasis [28]. In animals [29,30] and in human bone marrow stem cells [31] Hcy also reduces expression of VEGF-A, VEGF and VEGFR-2. Reducing Hcy with B vitamins therefore might sometimes increase VEGF.

TNF-alpha can enhance iNOS mRNA and NO in mice [32]. Blocking TNF-alpha therefore might reduce NO. In mice, up regulation of iNOSmRNA appears to lead to increased NOS and increased superoxide generation which might cause cardiac ischemia and secondary enlargement [33]. Blocking TNF-alpha therefore should have been cardio –protective, but in one one subject left axis deviation appeared on adalimumab alone and in a second it appeared after high dose folic acid, B6 and B12 were added to the adalimumab.

CONCLUSION

Five mg daily folic acid plus B6 and B12 and adalimumab worsens psoriasis in our subjects with low BMIs. EKG changes may be an impediment for use in patient with higher BMIs. Addition of these vitamins may not improve psoriasis over the effects of adalimumab alone. The combination of adalimumab and high dose folic acid cannot for now be recommended.

ACKNOWLEDGEMENTS

Sharon Click, Sara Herman, M.D,Susan Menard, R.N.EmmaReaves,Chen Young, Ph. D.,Jinmeng Zhang, M.D. Grant Details: Funding from Abbott Pharmaceuticals (now ABBVIE): Investigator initiated Study: A single center open-label study to Assess the effects of the addition of modulators of homocysteine to adalimumab in the treatment of moderate to severe plaque psoriasis. Abbott Pharmaceuticals, HUM 07-035. clinicaltrials. gov NCT10704599.

Conflict of Interest

Aronson Peter J: Investigator initiated Study: A single center open-label study to Assess the effects of the addition of modulators of homocysteine to adalimumab in the treatment of moderate to severe plaque psoriasis. Abbott Pharmaceuticals, HUM 07-035. clinicaltrials.gov NCT10704599. OBSERVE 5: 5 year safety study of etanercept for moderate to severe plaque Psoriasis.Amgen. Clinicaltrials.gov. NCT00322439. (sinceetanercept is functionally similar to adalimnumab and effects of this drug are mentioned in the text of this article).

All cases using adalimumab were funded by a grant by Abbott, now ABBVIE with funding by them to the Department of Dermatology, Wayne State University. Investigators on this study were Drs. Aronson, Swearingen, Malick, and both Drs. Mehregan. The funding to the Wayne State Department of Dermatology was from Amgen with investigators being Drs. Aronson and Malick. Wayne State University paid and employed all investigators.

Dr. Aronson personally has psoriasis and it has flared on 1-2 mg folic acid but has cleared twice on 5-7 mg folic acid without biologicals.(why is may be conflict of interest: www.news. harvard.edu/gazette/2004/04.29/11-selfexperiment.htm)..

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Aronson PJ, Swearingen J, Malick F, Mehregan DA, Mehregan DR (2015) High dose Folic Acid Plus Vitamins B6 and B12 Added to Adalimumab can Worsen Psoriasis In Patients with Low BMIs and in Others May Cause Unanticipated EKG Effects. J Dermatolog Clin Res 3(1): 1039.

Received : 03 Dec 2014
Accepted : 04 Jan 2015
Published : 07 Jan 2015
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Annals of Mens Health and Wellness
ISSN : 2641-7707
Launched : 2017
Journal of Preventive Medicine and Health Care
ISSN : 2576-0084
Launched : 2018
Journal of Chronic Diseases and Management
ISSN : 2573-1300
Launched : 2016
Annals of Vaccines and Immunization
ISSN : 2378-9379
Launched : 2014
JSM Heart Surgery Cases and Images
ISSN : 2578-3157
Launched : 2016
Annals of Reproductive Medicine and Treatment
ISSN : 2573-1092
Launched : 2016
JSM Brain Science
ISSN : 2573-1289
Launched : 2016
JSM Biomarkers
ISSN : 2578-3815
Launched : 2014
JSM Biology
ISSN : 2475-9392
Launched : 2016
Archives of Stem Cell and Research
ISSN : 2578-3580
Launched : 2014
Annals of Clinical and Medical Microbiology
ISSN : 2578-3629
Launched : 2014
JSM Pediatric Surgery
ISSN : 2578-3149
Launched : 2017
Journal of Memory Disorder and Rehabilitation
ISSN : 2578-319X
Launched : 2016
JSM Tropical Medicine and Research
ISSN : 2578-3165
Launched : 2016
JSM Head and Face Medicine
ISSN : 2578-3793
Launched : 2016
JSM Cardiothoracic Surgery
ISSN : 2573-1297
Launched : 2016
JSM Bone and Joint Diseases
ISSN : 2578-3351
Launched : 2017
JSM Bioavailability and Bioequivalence
ISSN : 2641-7812
Launched : 2017
JSM Atherosclerosis
ISSN : 2573-1270
Launched : 2016
Journal of Genitourinary Disorders
ISSN : 2641-7790
Launched : 2017
Journal of Fractures and Sprains
ISSN : 2578-3831
Launched : 2016
Journal of Autism and Epilepsy
ISSN : 2641-7774
Launched : 2016
Annals of Marine Biology and Research
ISSN : 2573-105X
Launched : 2014
JSM Health Education & Primary Health Care
ISSN : 2578-3777
Launched : 2016
JSM Communication Disorders
ISSN : 2578-3807
Launched : 2016
Annals of Musculoskeletal Disorders
ISSN : 2578-3599
Launched : 2016
Annals of Virology and Research
ISSN : 2573-1122
Launched : 2014
JSM Renal Medicine
ISSN : 2573-1637
Launched : 2016
Journal of Muscle Health
ISSN : 2578-3823
Launched : 2016
JSM Genetics and Genomics
ISSN : 2334-1823
Launched : 2013
JSM Anxiety and Depression
ISSN : 2475-9139
Launched : 2016
Clinical Journal of Heart Diseases
ISSN : 2641-7766
Launched : 2016
Annals of Medicinal Chemistry and Research
ISSN : 2378-9336
Launched : 2014
JSM Pain and Management
ISSN : 2578-3378
Launched : 2016
JSM Women's Health
ISSN : 2578-3696
Launched : 2016
Clinical Research in HIV or AIDS
ISSN : 2374-0094
Launched : 2013
Journal of Endocrinology, Diabetes and Obesity
ISSN : 2333-6692
Launched : 2013
Journal of Substance Abuse and Alcoholism
ISSN : 2373-9363
Launched : 2013
JSM Neurosurgery and Spine
ISSN : 2373-9479
Launched : 2013
Journal of Liver and Clinical Research
ISSN : 2379-0830
Launched : 2014
Journal of Drug Design and Research
ISSN : 2379-089X
Launched : 2014
JSM Clinical Oncology and Research
ISSN : 2373-938X
Launched : 2013
JSM Bioinformatics, Genomics and Proteomics
ISSN : 2576-1102
Launched : 2014
JSM Chemistry
ISSN : 2334-1831
Launched : 2013
Journal of Trauma and Care
ISSN : 2573-1246
Launched : 2014
JSM Surgical Oncology and Research
ISSN : 2578-3688
Launched : 2016
Annals of Food Processing and Preservation
ISSN : 2573-1033
Launched : 2016
Journal of Radiology and Radiation Therapy
ISSN : 2333-7095
Launched : 2013
JSM Physical Medicine and Rehabilitation
ISSN : 2578-3572
Launched : 2016
Annals of Clinical Pathology
ISSN : 2373-9282
Launched : 2013
Annals of Cardiovascular Diseases
ISSN : 2641-7731
Launched : 2016
Journal of Behavior
ISSN : 2576-0076
Launched : 2016
Annals of Clinical and Experimental Metabolism
ISSN : 2572-2492
Launched : 2016
Clinical Research in Infectious Diseases
ISSN : 2379-0636
Launched : 2013
JSM Microbiology
ISSN : 2333-6455
Launched : 2013
Journal of Urology and Research
ISSN : 2379-951X
Launched : 2014
Journal of Family Medicine and Community Health
ISSN : 2379-0547
Launched : 2013
Annals of Pregnancy and Care
ISSN : 2578-336X
Launched : 2017
JSM Cell and Developmental Biology
ISSN : 2379-061X
Launched : 2013
Annals of Aquaculture and Research
ISSN : 2379-0881
Launched : 2014
Clinical Research in Pulmonology
ISSN : 2333-6625
Launched : 2013
Journal of Immunology and Clinical Research
ISSN : 2333-6714
Launched : 2013
Annals of Forensic Research and Analysis
ISSN : 2378-9476
Launched : 2014
JSM Biochemistry and Molecular Biology
ISSN : 2333-7109
Launched : 2013
Annals of Breast Cancer Research
ISSN : 2641-7685
Launched : 2016
Annals of Gerontology and Geriatric Research
ISSN : 2378-9409
Launched : 2014
Journal of Sleep Medicine and Disorders
ISSN : 2379-0822
Launched : 2014
JSM Burns and Trauma
ISSN : 2475-9406
Launched : 2016
Chemical Engineering and Process Techniques
ISSN : 2333-6633
Launched : 2013
Annals of Clinical Cytology and Pathology
ISSN : 2475-9430
Launched : 2014
JSM Allergy and Asthma
ISSN : 2573-1254
Launched : 2016
Journal of Neurological Disorders and Stroke
ISSN : 2334-2307
Launched : 2013
Annals of Sports Medicine and Research
ISSN : 2379-0571
Launched : 2014
JSM Sexual Medicine
ISSN : 2578-3718
Launched : 2016
Annals of Vascular Medicine and Research
ISSN : 2378-9344
Launched : 2014
JSM Biotechnology and Biomedical Engineering
ISSN : 2333-7117
Launched : 2013
Journal of Hematology and Transfusion
ISSN : 2333-6684
Launched : 2013
JSM Environmental Science and Ecology
ISSN : 2333-7141
Launched : 2013
Journal of Cardiology and Clinical Research
ISSN : 2333-6676
Launched : 2013
JSM Nanotechnology and Nanomedicine
ISSN : 2334-1815
Launched : 2013
Journal of Ear, Nose and Throat Disorders
ISSN : 2475-9473
Launched : 2016
JSM Ophthalmology
ISSN : 2333-6447
Launched : 2013
Journal of Pharmacology and Clinical Toxicology
ISSN : 2333-7079
Launched : 2013
Annals of Psychiatry and Mental Health
ISSN : 2374-0124
Launched : 2013
Medical Journal of Obstetrics and Gynecology
ISSN : 2333-6439
Launched : 2013
Annals of Pediatrics and Child Health
ISSN : 2373-9312
Launched : 2013
JSM Clinical Pharmaceutics
ISSN : 2379-9498
Launched : 2014
JSM Foot and Ankle
ISSN : 2475-9112
Launched : 2016
JSM Alzheimer's Disease and Related Dementia
ISSN : 2378-9565
Launched : 2014
Journal of Addiction Medicine and Therapy
ISSN : 2333-665X
Launched : 2013
Journal of Veterinary Medicine and Research
ISSN : 2378-931X
Launched : 2013
Annals of Public Health and Research
ISSN : 2378-9328
Launched : 2014
Annals of Orthopedics and Rheumatology
ISSN : 2373-9290
Launched : 2013
Journal of Clinical Nephrology and Research
ISSN : 2379-0652
Launched : 2014
Annals of Community Medicine and Practice
ISSN : 2475-9465
Launched : 2014
Annals of Biometrics and Biostatistics
ISSN : 2374-0116
Launched : 2013
JSM Clinical Case Reports
ISSN : 2373-9819
Launched : 2013
Journal of Cancer Biology and Research
ISSN : 2373-9436
Launched : 2013
Journal of Surgery and Transplantation Science
ISSN : 2379-0911
Launched : 2013
Journal of Dermatology and Clinical Research
ISSN : 2373-9371
Launched : 2013
JSM Gastroenterology and Hepatology
ISSN : 2373-9487
Launched : 2013
Annals of Nursing and Practice
ISSN : 2379-9501
Launched : 2014
JSM Dentistry
ISSN : 2333-7133
Launched : 2013
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