Loading

Annals of Clinical and Experimental Hypertension

Oxidative Stress and Mineralocorticoid Receptor Signaling in the Brain: Possible Therapeutic Targets for Dementia

Review Article | Open Access
Article DOI :

  • 1. Division of Clinical Epigenetics ,University of Tokyo, Japan
  • 2. Department of Clinical Laboratory, University of Tokyo Faculty of Medicine, Japan
+ Show More - Show Less
Corresponding Authors
Tatsuo Shimosawa, 113-8655 7-3-1, Hongo, Bunkyo-Ku, Tokyo, Japan, Tel: 81-33815-5411
Abstract

Glucocorticoid and mineralocorticoid receptor signals are important for memory formation, salt cravings, sympathetic tone and hypothalamic–pituitary–adrenal (HPA) axis control in the brain. Exacerbations of glucocorticoid and mineralocorticoid receptor signaling cause atherosclerosis, cognitive dysfunction, and depression. Mineralocorticoid activity is modulated by oxidative stress, and chronic stress desensitize further nongenomic mineralocorticoid receptor action. In patients with chronic diseases such as diabetes and hypertension, oxidative stress in the brain is increased, which alters the balance of the HPA axis, resulting in an elevated risk of dementia. Therefore, oxidative stress and mineralocorticoid receptor blockade are possible therapeutic targets for cognitive dysfunction.

Keywords

• Mineralocorticoid receptor

• Oxidative stress

• Brain damage

• Hypertension

Citation

Kawakami-Mori F, Shimosawa T (2014) Oxidative Stress and Mineralocorticoid Receptor Signaling in the Brain: Possible Therapeutic Targets for Dementia. Ann Clin Exp Hypertension 2(2): 1015.

INTRODUCTION

There is compelling evidence that diabetes mellitus, hypertension, ischemic brain injury, and psychiatric disease cause oxidative stress in the brain leading to cognitive deficits and dementia[1-3].The development of effective treatments for improving cognitive function is of great public health concern because of the high prevalence of hypertension and diabetes in the growing elderly population. The association between hypertension and cognitive decline has been of particular interest and there have been several clinical trials of antihypertensive drugs in the elderly, such as SCOPE, PROGRESS, HYVET, Sys-Eur [4,5];among these, only the Sys-Eur trial showed any benefit of antihypertensive drugs on preservation of cognitive function. However, these studies were limited by the relatively short observation periods and initiation of treatment late in the disease process, which precluded the ability to observe any meaningful impact on cognitive decline. In support, a recently published20- year large-scale retrospective clinical trial showed that midlife hypertension was strongly associated with cognitive decline in later life, and early intervention with antihypertensive drugs could ameliorate this process [6].High blood pressure causes cognitive decline after a long time, therefore early intervention is critical to prevent disease progression. In spite of these findings, to date there are no adequate data to indicate which antihypertensive agent is most effective. In the brain, neurotransmitters are secreted from the presynapse and bind mainly to postsynaptic receptors to open ionic channels and modulate the activity of postsynaptic cells. Glutamate is an excitatory neurotransmitter that induces cation entry by activation of the α-amino-3-hydroxy-5-methyl4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors on glial cells. Glutamate plays an important role in learning and in establishing memory. Meanwhile, excessive secretion of glutamate induces cell vulnerability and/ or cell death by over-excitation [7].Glutamate release is regulated by many neuro modulators that are affected by chronic diseases such as cerebral ischemia, diabetes, and hypertension. Here, we review the role of the neuro modulator glucocorticoid (GC) and oxidative stress in the development and progression of dementia.

Steroid receptor distribution and effect in the brain

There are many ligands and receptors on neurons, in addition to neurotransmitter receptors, that modulate transmitter release and/or ionic channel activation. Among these, GC is a known neuromodulator that has an important role in cognitive memory, fear memory, and salt cravings [8, 9]. GCs bind two steroid receptors, glucocorticoid receptors (GRs) and mineralocorticoid receptors (MRs). In the brain, GRs are expressed ubiquitously, whereas MRs is localized to specific areas, such as the hippocampus, para ventricular nucleus, and brainstem. The binding affinity of corticosterone (CS) for MRs is 10-fold higher than GRs, and under normal conditions, CS preferentially binds to MRs. MRs bind not only aldosterone but also cortisol and CS with almost the same affinity. Under normal physiological conditions, the concentration of cortisol and CS is at least three orders of magnitude higher than aldosterone; therefore, the active ligands of MRs are mainly cortisol and CS in regions of the brain where 11β-hydroxysteroid dehydrogenase2(11βHSD2) is not expressed. 11βHSD2 metabolizes CS to its inactive form cortisone and in the brain, 11βHSD2 is expressed only in specific regions area such as the tractus solitarius, subcommisural organ, and ventromedial hypothalamus [10]. Hence, in most of the brain, the main MR agonist is CS.

For memory formation, GR and MR balance is important; for instance, GR is mainly for memory consolidation and MR is for memory acquisition [11].Both receptors are activated concurrently; an imbalance is associated with cognitive impairment and dysregulation of the hypothalamic–pituitary– adrenal (HPA) axis [12-14]. Imbalances can be induced by selective agonists such as dexamethasone, or chronic diseases that increase oxidative stress in the brain. Oxidative stress modulates mainly the MR signal. MRs act not only through classical transcriptional pathways, but also via short-term, non-genomic action through G-proteincoupled receptors (GPCRs). When circulating CS levels are increased by stress, CS binds membranous MRs, which are of lower affinity than intracellular MRs. These activate voltagedependent L-type Ca2+ channels and increase glutamate release from the presynapse, while simultaneously inactivating the postsynaptic K+ channel (IA current) within minutes in a nongenomic manner [15-18].

Relationship between oxidative stress and MR action

Figure 1 A) The representative data of field excitatory postsynaptic potentials fEPSPs in the CA1 region of the hippocampus induced by stimulation of the perforate pathway. B) Applying 100nM corticosterone (CS) and/or 1?M NADPH rapidly increased fEPSPs via non-genomic action. All data were obtained in the presence of 500 nM actinomycin D to confirm non-genomic action. Simultaneous application of 100nM CS and 1?M NADPH increased fEPSP remarkably. C) Non-genomic action by CS is ameliorated by an NADPH oxidase inhibitor (100?M apocynin) or Rac1 inhibitor (50?M NSC23766). These data are from (25).

Figure 1 A) The representative data of field excitatory postsynaptic potentials fEPSPs in the CA1 region of the hippocampus induced by stimulation of the perforate pathway. B) Applying 100nM corticosterone (CS) and/or 1μM NADPH rapidly increased fEPSPs via non-genomic action. All data were obtained in the presence of 500 nM actinomycin D to confirm non-genomic action. Simultaneous application of 100nM CS and 1μM NADPH increased fEPSP remarkably. C) Non-genomic action by CS is ameliorated by an NADPH oxidase inhibitor (100μM apocynin) or Rac1 inhibitor (50μM NSC23766). These data are from (25).

In peripheral tissue, MR controls sodium transport and induces inflammation, fibrosis, and hypertrophy. This action is independent of serum ligand concentration, and is potentiated by oxidative stress, which has been a paradoxical problem regarding MRs for a long time [19, 20]. One factor that exacerbates MR action under conditions of oxidative stress is 11βHSD1/2[19, 21, 22], and another is Rac1 (23, 24). 11βHSD1 converts cortisone to CS or deoxycorticosterone, which is NADPH-dependent and then11βHSD2 metabolize CS to cortisone, which is also NADPHdependent. Oxidative stress activates 11βHSD1 and inactivates 11βHSD2, resulting in increased concentrations of CS in the tissue. In contrast, Rac1 GTPase, a member of the Rho family GTPases, activates MR nuclear translocation, MR-dependent transcription, and MR-dependent signals such as serum and glucocorticoidregulated kinase 1(SgK1) [23,24]. Rac1 is dispensable for the non-genomic action of MR in the brain. The Rac1 inhibitor, NSC23766, and the NADPH oxidase inhibitor apocynin block the non-genomic action of CS, rapid potentiation of field excitatory postsynaptic potential (EPSP), and ERK1/2 phosphorylation in hippocampal neurons. Furthermore, simultaneous stimulation of MR and NADPH oxidase increases potentiation of field EPSPs (Figure 1) and ERK pathway activation remarkably. Meanwhile, the non-genomic action of MR activates Rac1 GTP, and induces MR translocation (Figure2). These results indicate that both CS elevation and oxidative stress induce MR activation, and oxidative stress is subsequently further increased by MR activation [24] in a feedback loop. The physiological roles of non-genomic action of MRs include memory retrieval [26-29], negative feedback with the HPA axis via hippocampal activation, suppression of adrenocorticotropic hormone(ACTH) and CS [30],and possibly ,effects on transition to genomic action [31]. It is plausible that oxidative stress modulates the physiological functions of the non-genomic action of MR leading to pathological consequences of cognitive dysfunction and multiple organ dysfunction.

Figure 2 A) Ten min after addition of corticosterone (CS) and/or NADPH, Rac1 GTPase was activated in the CA1 region of the hippocampus. CS and NADPH had an additive effect. B) Rac1 activation by CS and/or NADPH was blocked by 1?M spironolactone, apocynin, and Tempol. C) Rac1 is a key factor in the feedback loop between mineralocorticoid receptors (MR) and NADPH oxidase.

Figure 2 A) Ten min after addition of corticosterone (CS) and/or NADPH, Rac1 GTPase was activated in the CA1 region of the hippocampus. CS and NADPH had an additive effect. B) Rac1 activation by CS and/or NADPH was blocked by 1μM spironolactone, apocynin, and Tempol. C) Rac1 is a key factor in the feedback loop between mineralocorticoid receptors (MR) and NADPH oxidase.

MR blockade in the brain and cognitive function

GC elevation is commonly observed in patients with poorly controlled diabetes and depression. In fetuses, a high salt diet changes glucocorticoid metabolism in the tissues and affects the HPA axis [32,33]. HPA axis dysregulation and/or set point changes are partially induced by oxidative stress; glucocorticoid excess further induces oxidative stress via the MR pathway and glutamate release in the brain, causing neuron damage. A mouse model of diabetes showed impaired memory; treatment of these mice with an MR blocker that can pass through blood brain barrier, protected cognitive functions [34]. On the other hand, treatment with a high dose of MR blocker impaired selective attention and working memory performance but enhanced long term memory in healthy humans [35,36].It is possible that MR blockade increased serum cortisol and changed the MR/GR activation ratio[37].

Figure 3 Chronic oxidative stress and excess corticosterone (CS) induce mineralocorticoid receptor (MR) activation that leads to memory impairment.

Figure 3 Chronic oxidative stress and excess corticosterone (CS) induce mineralocorticoid receptor (MR) activation that leads to memory impairment.

Rac1 inhibition and cognitive function

In our study, a Rac1 inhibitor attenuated the non-genomic action of MR and NADPH in the CA1 region of the hippocampus [25].While Rac1 GTPase is dispensable for NADPH oxidase activation, Rac1 inhibition prevented neuronal degeneration caused by oxidative stress [38], reduced reactive oxygen species (ROS) generation, and oxidative stress in the hippocampal CA1 region, and delayed neuronal cell death after ischemic injury [39]. Rac1 inhibition also increased long term potentiation in the CA1 region [40]. Furthermore, comparing Rac1 and Rho A inhibition [41-43], Rac1 blockade appears to have a protective role in preventing brain damage, positively affecting cognitive function, and therefore, is a potential therapeutic target to prevent diseases caused chronic oxidative stress.

 

 

DISCUSSION

The pathophysiological roles of MR in cognitive function have been studied. We could step forward to translate these findings to diagnosis and therapy in clinical settings. Although there are no data delineating which antihypertensive drug is the most effective to prevent cognitive dysfunction, considering the action of MR in the brain, antihypertensive drugs that are also MR blockers could be possible therapeutics for dementia. Further, Rac1 inhibitors might be additional therapeutic candidates because they inhibit chronic ROS generation in the brain in diabetes, hypertension, and mental illness disease models. The further study of this field is expected to contribute to improve mental and physical health of aged people.

ACKNOWLEDGEMENTS

The study of ref [25] was supported by grants of 24790834 from KAKEN in Japan.

REFERENCES

1. Cukierman T, Gerstein HC, Williamson JD. Cognitive decline and dementia in diabetes--systematic overview of prospective observational studies. Diabetologia. 2005; 48: 2460-2469.

2. Mogi N, Umegaki H, Hattori A, Maeda N, Miura H, Kuzuya M ,et al. Cognitive function in Japanese elderly with type 2 diabetes mellitus. J Diabetes Complications. 2004; 18: 42-46.

3. Umegaki H, Iimuro S, Shinozaki T, Araki A, Sakurai T, Iijima K, et al. Risk factors associated with cognitive decline in the elderly with type 2 diabetes: pooled logistic analysis of a 6-year observation in the Japanese Elderly Diabetes Intervention Trial. Geriatr Gerontol Int. 2012; 12 Suppl 1:110-116.

4. Peters R, Beckett N, Forette F, Tuomilehto J, Clarke R, Ritchie C, et al. Incident dementia and blood pressure lowering in the Hypertension in the Very Elderly Trial cognitive function assessment (HYVET-COG): a double-blind, placebo controlled trial. Lancet Neurol. 2008; 7 :683- 689.

5. Forette F, Seux ML, Staessen JA, Thijs L, Birkenhäger WH, Babarskiene MR,et al. Prevention of dementia in randomised double-blind placebocontrolled Systolic Hypertension in Europe (Syst-Eur) trial. Lancet. 1998; 352: 1347-1351.

6. Gottesman RF, Schneider AL, Albert M, Alonso A, Bandeen-Roche K, Coker L, et al. Midlife Hypertension and 20-Year Cognitive Change: The Atherosclerosis Risk in Communities Neurocognitive Study. JAMA Neurol. 2014;71:1218-1287.

7. Schubert D, Piasecki D. Oxidative glutamate toxicity can be a component of the excitotoxicity cascade. J Neurosci. 2001; 21: 7455- 7462.

8. Coirini H, Magarinos AM, De Nicola AF, Rainbow TC, McEwen BS. Further studies of brain aldosterone binding sites employing new mineralocorticoid and glucocorticoid receptor markers in vitro. Brain Res. 1985;361:212-216.

9. Rozeboom AM, Akil H, Seasholtz AF. Mineralocorticoid receptor overexpression in forebrain decreases anxiety-like behavior and alters the stress response in mice. Proc Natl Acad Sci U S A. 2007; 104: 4688-4693.

10.Geerling JC, Loewy AD. Aldosterone in the brain. Am J Physiol Renal Physiol. 2009; 297: F559-576.

11.Oitzl MS, de Kloet ER. Selective corticosteroid antagonists modulate specific aspects of spatial orientation learning. Behav Neurosci. 1992; 106: 62-71.

12.Rogalska J. Mineralocorticoid and glucocorticoid receptors in hippocampus: their impact on neurons survival and behavioral impairment after neonatal brain injury. Vitam Horm. 2010;82: 391- 419.

13.Kellendonk C, Gass P, Kretz O, Schütz G, Tronche F. Corticosteroid receptors in the brain: gene targeting studies. Brain Res Bull. 2002; 57: 73-83.

14.Rybnikova E, Glushchenko T, Churilova A, Pivina S, Samoilov M. Expression of glucocorticoid and mineralocorticoid receptors in hippocampus of rats exposed to various modes of hypobaric hypoxia: Putative role in hypoxic preconditioning. Brain Res. 2011;1381:66-77.

15.Callera GE, Touyz RM, Tostes RC, Yogi A, He Y, Malkinson S, Schiffrin EL. Aldosterone activates vascular p38MAP kinase and NADPH oxidase via c-Src. Hypertension. 2005; 45: 773-779.

16. Chameau P, Qin Y, Spijker S, Smit AB, Smit G, Joëls M. Glucocorticoids specifically enhance L-type calcium current amplitude and affect calcium channel subunit expression in the mouse hippocampus. J Neurophysiol. 2007;97: 5-14.

17.Karst H, Berger S, Turiault M, Tronche F, Schütz G, Joëls M. Mineralocorticoid receptors are indispensable for nongenomic modulation of hippocampal glutamate transmission by corticosterone. Proc Natl Acad Sci U S A. 2005;102 :19204-19207.

18.Olijslagers JE, de Kloet ER, Elgersma Y, van Woerden GM, Joëls M, Karst H. Rapid changes in hippocampal CA1 pyramidal cell function via preas well as postsynaptic membrane mineralocorticoid receptors. Eur J Neurosci. 2008;27:2542-50.

19.Funder JW. Mineralocorticoid receptor activation and oxidative stress. Hypertension. 2007; 50: 840-841.

20.Young MJ , Moussa L, Dilley R, Funder JW. Early inflammatory responses in experimental cardiac hypertrophy and fibrosis: effects of 11 beta-hydroxysteroid dehydrogenase inactivation. Endocrinology. 2003; 144: 1121-1125.

21.Hewitt KN, Walker EA, Stewart PM. Minireview: hexose-6-phosphate dehydrogenase and redox control of 11{beta}-hydroxysteroid dehydrogenase type 1 activity. Endocrinology. 2005;146:2539-2543.

22.Tsugita M, Iwasaki Y, Nishiyama M, Taguchi T, Shinahara M, Taniguchi Y, et al. Differential regulation of 11beta-hydroxysteroid dehydrogenase type-1 and -2 gene transcription by proinflammatory cytokines in vascular smooth muscle cells. Life Sci. 2008;83:426-32.

23.Shibata S, Nagase M, Yoshida S, Kawarazaki W, Kurihara H, Tanaka H, Miyoshi J. Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease. Nat Med. 2008; 14: 1370-1376.

24.Shibata S, Mu S, Kawarazaki H, Muraoka K, Ishizawa K, Yoshida S, et al. Rac1 GTPase in rodent kidneys is essential for salt-sensitive hypertension via a mineralocorticoid receptor-dependent pathway. J Clin Invest. 2011; 121:3233-3243.

25.Mori FK, Shimosawa T, Wang H, Ogura S, Mu S, Yatomi Y, et al. NADPH oxidase-mediated Rac1 GTP activity is necessary for non-genomic actions of the mineralocorticoid receptor in the CA1 region of the rat hippocampus. Am J Physiol Endocrinol Metab. 2011.

26.Groeneweg FL, Karst H, de Kloet ER, Joëls M. Rapid non-genomic effects of corticosteroids and their role in the central stress response. J Endocrinol. 2011; 209: 153-167.

27.Haller J, Halasz J, Mikics E, Kruk MR, Makara GB. Ultradian corticosterone rhythm and the propensity to behave aggressively in male rats. J Neuroendocrinol. 2000; 12:937-940.

28.Haller J, Mikics E, Makara GB. The effects of non-genomic glucocorticoid mechanisms on bodily functions and the central neural system. A critical evaluation of findings. Front Neuroendocrinol. 2008;29:273- 291.

29.Tasker JG, Di S, Malcher-Lopes R. Minireview: rapid glucocorticoid signaling via membrane-associated receptors. Endocrinology. 2006; 147: 5549-5556.

30.Atkinson HC, Wood SA, Castrique ES, Kershaw YM, Wiles CC, Lightman SL. Corticosteroids mediate fast feedback of the rat hypothalamicpituitary-adrenal axis via the mineralocorticoid receptor. Am J Physiol Endocrinol Metab. 2008;294:E1011-1022.

31.Di S, Malcher-Lopes R, Halmos KC, Tasker JG. Nongenomic glucocorticoid inhibition via endocannabinoid release in the hypothalamus: a fast feedback mechanism. J Neurosci. 2003; 23: 4850-4857.

32.Baudrand R, Campino C, Carvajal CA, Olivieri O, Guidi G, Faccini G, et al. High sodium intake is associated with increased glucocorticoid production, insulin resistance and metabolic syndrome. Clin Endocrinol (Oxf). 2014;80: 677-684.

33.Mao C, Liu R, Bo L, Chen N, Li S, Xia S, Chen J. High-salt diets during pregnancy affected fetal and offspring renal renin-angiotensin system. J Endocrinol. 2013; 218: 61-73.

34.Sakata A, Mogi M, Iwanami J, Tsukuda K, Min LJ, Jing F, Ohshima K. Improvement of cognitive impairment in female type 2 diabetes mellitus mice by spironolactone. J Renin Angiotensin Aldosterone Syst. 2012; 13: 84-90.

35.Cornelisse S, Joels M, Smeets T. A randomized trial on mineralocorticoid receptor blockade in men: effects on stress responses, selective attention, and memory. Neuropsychopharmacology. 2011; 36:2720- 2728.

36.Otte C, Moritz S, Yassouridis A, Koop M, Madrischewski AM, Wiedemann K, et al. Blockade of the mineralocorticoid receptor in healthy men: effects on experimentally induced panic symptoms, stress hormones, and cognition. Neuropsychopharmacology. 2007; 32:232-238.

37.Mattsson C, Reynolds RM, Simonyte K, Olsson T, Walker BR. Combined receptor antagonist stimulation of the hypothalamic-pituitary-adrenal axis test identifies impaired negative feedback sensitivity to cortisol in obese men. J Clin Endocrinol Metab. 2009;94:1347-1352.

38.Raz L, Zhang QG, Zhou CF, Han D, Gulati P, Yang LC, Yang F. Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat. PLoS One. 2010; 5: e12606.

39.Zhang QG, Wang R, Han D, Dong Y, Brann DW. Role of Rac1 GTPase in JNK signaling and delayed neuronal cell death following global cerebral ischemia. Brain Res. 2009; 1265: 138-147.

40.O’Kane EM, Stone TW, Morris BJ. Increased long-term potentiation in the CA1 region of rat hippocampus via modulation of GTPase signalling or inhibition of Rho kinase. Neuropharmacology. 2004; 46: 879-887.

41.Quinn M, McMillin M, Galindo C, Frampton G, Pae HY , DeMorrow S,.Bile acids permeabilize the blood brain barrier after bile duct ligation in rats via Rac1-dependent mechanisms. Dig Liver Dis. 2014; 46: 527- 534. 

42.Huang B, Krafft PR, Ma Q, Rolland WB, Caner B, Lekic T, et al. Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice. Neurobiol Dis. 2012;46:204-14.

43.Rochfort KD, Collins LE, Murphy RP, Cummins PM. Downregulation of blood-brain barrier phenotype by proinflammatory cytokines involves NADPH oxidase-dependent ROS generation: consequences for interendothelial adherens and tight junctions. PLoS One. 2014;9:e101815.

Kawakami-Mori F, Shimosawa T (2014) Oxidative Stress and Mineralocorticoid Receptor Signaling in the Brain: Possible Therapeutic Targets for Dementia. Ann Clin Exp Hypertension 2(2): 1015

Received : 10 Oct 2014
Accepted : 19 Nov 2014
Published : 28 Nov 2014
Journals
Annals of Otolaryngology and Rhinology
ISSN : 2379-948X
Launched : 2014
JSM Schizophrenia
Launched : 2016
Journal of Nausea
Launched : 2020
JSM Internal Medicine
Launched : 2016
JSM Hepatitis
Launched : 2016
JSM Oro Facial Surgeries
ISSN : 2578-3211
Launched : 2016
Journal of Human Nutrition and Food Science
ISSN : 2333-6706
Launched : 2013
JSM Regenerative Medicine and Bioengineering
ISSN : 2379-0490
Launched : 2013
JSM Spine
ISSN : 2578-3181
Launched : 2016
Archives of Palliative Care
ISSN : 2573-1165
Launched : 2016
JSM Nutritional Disorders
ISSN : 2578-3203
Launched : 2017
Annals of Neurodegenerative Disorders
ISSN : 2476-2032
Launched : 2016
Journal of Fever
ISSN : 2641-7782
Launched : 2017
JSM Bone Marrow Research
ISSN : 2578-3351
Launched : 2016
JSM Mathematics and Statistics
ISSN : 2578-3173
Launched : 2014
Journal of Autoimmunity and Research
ISSN : 2573-1173
Launched : 2014
JSM Arthritis
ISSN : 2475-9155
Launched : 2016
JSM Head and Neck Cancer-Cases and Reviews
ISSN : 2573-1610
Launched : 2016
JSM General Surgery Cases and Images
ISSN : 2573-1564
Launched : 2016
JSM Anatomy and Physiology
ISSN : 2573-1262
Launched : 2016
JSM Dental Surgery
ISSN : 2573-1548
Launched : 2016
Annals of Emergency Surgery
ISSN : 2573-1017
Launched : 2016
Annals of Mens Health and Wellness
ISSN : 2641-7707
Launched : 2017
Journal of Preventive Medicine and Health Care
ISSN : 2576-0084
Launched : 2018
Journal of Chronic Diseases and Management
ISSN : 2573-1300
Launched : 2016
Annals of Vaccines and Immunization
ISSN : 2378-9379
Launched : 2014
JSM Heart Surgery Cases and Images
ISSN : 2578-3157
Launched : 2016
Annals of Reproductive Medicine and Treatment
ISSN : 2573-1092
Launched : 2016
JSM Brain Science
ISSN : 2573-1289
Launched : 2016
JSM Biomarkers
ISSN : 2578-3815
Launched : 2014
JSM Biology
ISSN : 2475-9392
Launched : 2016
Archives of Stem Cell and Research
ISSN : 2578-3580
Launched : 2014
Annals of Clinical and Medical Microbiology
ISSN : 2578-3629
Launched : 2014
JSM Pediatric Surgery
ISSN : 2578-3149
Launched : 2017
Journal of Memory Disorder and Rehabilitation
ISSN : 2578-319X
Launched : 2016
JSM Tropical Medicine and Research
ISSN : 2578-3165
Launched : 2016
JSM Head and Face Medicine
ISSN : 2578-3793
Launched : 2016
JSM Cardiothoracic Surgery
ISSN : 2573-1297
Launched : 2016
JSM Bone and Joint Diseases
ISSN : 2578-3351
Launched : 2017
JSM Bioavailability and Bioequivalence
ISSN : 2641-7812
Launched : 2017
JSM Atherosclerosis
ISSN : 2573-1270
Launched : 2016
Journal of Genitourinary Disorders
ISSN : 2641-7790
Launched : 2017
Journal of Fractures and Sprains
ISSN : 2578-3831
Launched : 2016
Journal of Autism and Epilepsy
ISSN : 2641-7774
Launched : 2016
Annals of Marine Biology and Research
ISSN : 2573-105X
Launched : 2014
JSM Health Education & Primary Health Care
ISSN : 2578-3777
Launched : 2016
JSM Communication Disorders
ISSN : 2578-3807
Launched : 2016
Annals of Musculoskeletal Disorders
ISSN : 2578-3599
Launched : 2016
Annals of Virology and Research
ISSN : 2573-1122
Launched : 2014
JSM Renal Medicine
ISSN : 2573-1637
Launched : 2016
Journal of Muscle Health
ISSN : 2578-3823
Launched : 2016
JSM Genetics and Genomics
ISSN : 2334-1823
Launched : 2013
JSM Anxiety and Depression
ISSN : 2475-9139
Launched : 2016
Clinical Journal of Heart Diseases
ISSN : 2641-7766
Launched : 2016
Annals of Medicinal Chemistry and Research
ISSN : 2378-9336
Launched : 2014
JSM Pain and Management
ISSN : 2578-3378
Launched : 2016
JSM Women's Health
ISSN : 2578-3696
Launched : 2016
Clinical Research in HIV or AIDS
ISSN : 2374-0094
Launched : 2013
Journal of Endocrinology, Diabetes and Obesity
ISSN : 2333-6692
Launched : 2013
Journal of Substance Abuse and Alcoholism
ISSN : 2373-9363
Launched : 2013
JSM Neurosurgery and Spine
ISSN : 2373-9479
Launched : 2013
Journal of Liver and Clinical Research
ISSN : 2379-0830
Launched : 2014
Journal of Drug Design and Research
ISSN : 2379-089X
Launched : 2014
JSM Clinical Oncology and Research
ISSN : 2373-938X
Launched : 2013
JSM Bioinformatics, Genomics and Proteomics
ISSN : 2576-1102
Launched : 2014
JSM Chemistry
ISSN : 2334-1831
Launched : 2013
Journal of Trauma and Care
ISSN : 2573-1246
Launched : 2014
JSM Surgical Oncology and Research
ISSN : 2578-3688
Launched : 2016
Annals of Food Processing and Preservation
ISSN : 2573-1033
Launched : 2016
Journal of Radiology and Radiation Therapy
ISSN : 2333-7095
Launched : 2013
JSM Physical Medicine and Rehabilitation
ISSN : 2578-3572
Launched : 2016
Annals of Clinical Pathology
ISSN : 2373-9282
Launched : 2013
Annals of Cardiovascular Diseases
ISSN : 2641-7731
Launched : 2016
Journal of Behavior
ISSN : 2576-0076
Launched : 2016
Annals of Clinical and Experimental Metabolism
ISSN : 2572-2492
Launched : 2016
Clinical Research in Infectious Diseases
ISSN : 2379-0636
Launched : 2013
JSM Microbiology
ISSN : 2333-6455
Launched : 2013
Journal of Urology and Research
ISSN : 2379-951X
Launched : 2014
Journal of Family Medicine and Community Health
ISSN : 2379-0547
Launched : 2013
Annals of Pregnancy and Care
ISSN : 2578-336X
Launched : 2017
JSM Cell and Developmental Biology
ISSN : 2379-061X
Launched : 2013
Annals of Aquaculture and Research
ISSN : 2379-0881
Launched : 2014
Clinical Research in Pulmonology
ISSN : 2333-6625
Launched : 2013
Journal of Immunology and Clinical Research
ISSN : 2333-6714
Launched : 2013
Annals of Forensic Research and Analysis
ISSN : 2378-9476
Launched : 2014
JSM Biochemistry and Molecular Biology
ISSN : 2333-7109
Launched : 2013
Annals of Breast Cancer Research
ISSN : 2641-7685
Launched : 2016
Annals of Gerontology and Geriatric Research
ISSN : 2378-9409
Launched : 2014
Journal of Sleep Medicine and Disorders
ISSN : 2379-0822
Launched : 2014
JSM Burns and Trauma
ISSN : 2475-9406
Launched : 2016
Chemical Engineering and Process Techniques
ISSN : 2333-6633
Launched : 2013
Annals of Clinical Cytology and Pathology
ISSN : 2475-9430
Launched : 2014
JSM Allergy and Asthma
ISSN : 2573-1254
Launched : 2016
Journal of Neurological Disorders and Stroke
ISSN : 2334-2307
Launched : 2013
Annals of Sports Medicine and Research
ISSN : 2379-0571
Launched : 2014
JSM Sexual Medicine
ISSN : 2578-3718
Launched : 2016
Annals of Vascular Medicine and Research
ISSN : 2378-9344
Launched : 2014
JSM Biotechnology and Biomedical Engineering
ISSN : 2333-7117
Launched : 2013
Journal of Hematology and Transfusion
ISSN : 2333-6684
Launched : 2013
JSM Environmental Science and Ecology
ISSN : 2333-7141
Launched : 2013
Journal of Cardiology and Clinical Research
ISSN : 2333-6676
Launched : 2013
JSM Nanotechnology and Nanomedicine
ISSN : 2334-1815
Launched : 2013
Journal of Ear, Nose and Throat Disorders
ISSN : 2475-9473
Launched : 2016
JSM Ophthalmology
ISSN : 2333-6447
Launched : 2013
Journal of Pharmacology and Clinical Toxicology
ISSN : 2333-7079
Launched : 2013
Annals of Psychiatry and Mental Health
ISSN : 2374-0124
Launched : 2013
Medical Journal of Obstetrics and Gynecology
ISSN : 2333-6439
Launched : 2013
Annals of Pediatrics and Child Health
ISSN : 2373-9312
Launched : 2013
JSM Clinical Pharmaceutics
ISSN : 2379-9498
Launched : 2014
JSM Foot and Ankle
ISSN : 2475-9112
Launched : 2016
JSM Alzheimer's Disease and Related Dementia
ISSN : 2378-9565
Launched : 2014
Journal of Addiction Medicine and Therapy
ISSN : 2333-665X
Launched : 2013
Journal of Veterinary Medicine and Research
ISSN : 2378-931X
Launched : 2013
Annals of Public Health and Research
ISSN : 2378-9328
Launched : 2014
Annals of Orthopedics and Rheumatology
ISSN : 2373-9290
Launched : 2013
Journal of Clinical Nephrology and Research
ISSN : 2379-0652
Launched : 2014
Annals of Community Medicine and Practice
ISSN : 2475-9465
Launched : 2014
Annals of Biometrics and Biostatistics
ISSN : 2374-0116
Launched : 2013
JSM Clinical Case Reports
ISSN : 2373-9819
Launched : 2013
Journal of Cancer Biology and Research
ISSN : 2373-9436
Launched : 2013
Journal of Surgery and Transplantation Science
ISSN : 2379-0911
Launched : 2013
Journal of Dermatology and Clinical Research
ISSN : 2373-9371
Launched : 2013
JSM Gastroenterology and Hepatology
ISSN : 2373-9487
Launched : 2013
Annals of Nursing and Practice
ISSN : 2379-9501
Launched : 2014
JSM Dentistry
ISSN : 2333-7133
Launched : 2013
Author Information X