Biology of Cancellous Bone Graft Materials and their Usage for Bone Regeneration
- 1. Department of Genetics and Bioengineering, Yeditepe University, Turkey
ABSTRACT
Bone grafting have been used to treat nonunion, union and acute fractures. Autologous cancellous bone grafts are still the most effective graft material for stimulating bone repair since they present osteoconduction, osteoinduction and osteogenic capacity that are mainly requirements for bone regeneration. However, there are several negative aspects of autologous cancellous grafts such as additional surgical site, post-operative complications and inadequate amount of grafts. Allogeneic cancellous bone grafts, on the other hand, have the same characteristics as cancellous autograft with the exclusion of osteogenic capacity. Understanding of various biological processes such as host mediated immune response, osteo-integration of the graft within host and new bone re-modelling leads to improve the new biological strategies for endosseous grafts. We’ll discuss the biology of the cancellous bone graft which is essential to understand host-graft incorporate and also evaluate the specific factors associated with the osseous healing around the graft.
KEYWORDS
• Cancellous bone graft
• Orthopedic research
• Osteoinduction
• Graft biology
CITATION
Atasoy A, Kose GT (2016) Biology of Cancellous Bone Graft Materials and their Usage for Bone Regeneration. JSM Biotechnol Bioeng 3(2): 1051
ABBREVIATIONS
MSCs: Mesenchymal Stem Cells; DBM: Demineralized Bone Matrix; PRP: Platelet Rich Plasma; HA: Hydroxyapatite; TCP: Tricalcium Phosphate; IP-CHA: Interconnected Porous Calcium Hydroxyapatite ceramics; PLA-PEG: Polymer poly D,L,-lactic acidpolyethyleneglycol block co-polymer; BMPs: Bone Morphogenetic Proteins; rhBMPs: Recombinant Bone Morphogenetic Proteins; PDGFs: Platelets include Platelet Derived Growth Factors; TGF-β: Transforming Growth Factor-beta; VEGF: Vascular Endothelial Growth Factor; EGF: Epidermal Growth Factor; IGFs: Insulin-like Growth Factors; b-FGF: Basic Fibroblast Growth Factor; AATB: American Association of Tissue Banks; FDA: The U.S. Food and Drug Administration.
INTRODUCTION
Bone tissue can regenerate and repair itself. However, in some cases such as massive bone defects or pathological fractures, bone tissue can’t heal itself resulting in delayed unions or non-union, bone lesions and fractures with bone loss. Bone grafting is usually necessary when there is a great loss of healthy bone substance. After a grave injury, a tumor operation, or when an artificial joint is replaced, these grafts contribute towards the rapid anatomical and physiological restoration of tissue defects in patients. Appropriate bone graft material is generally selected taking into account several factors such as defect size, graft shape and their biological and mechanical features, preservation techniques and graft handling. Bone grafts are widely used in orthopedic field such as oral maxillofacial, reconstructive surgery, musculoskeletal injuries and sports medicine involving bone from minor defects to major bone loss [1].
BONE GRAFT PROPERTIES
Bone grafts show biological and mechanical features and provide a scaffolding so that new bone can be formed through osteogenesis, osteoinduction, and osteoconduction [2,3]. Graft materials should have at least two of these biological properties. Osteoconduction is described as one of the feature of bone graft that provides three dimensional scaffold for osteoblasts, facilitate vascularization and provide the migration of new host cells with osteogenic activity. Incorporation of bone graft materials depends on host surrounding viable tissue. Mechanical and biological properties of host-graft interface should permit the integration of the graft with the local host bone for successful osteogenic activity. As a new bone is formed, the graft could be partially or entirely resorbed through the bone formation process [4-6].
Osteoinduction is defined as the improvement of new bone formation in which MSCs are gathered from the host tissue and differentiated into bone cells by the stimulation of new bone productions such as bone proteins, growth factors and cytokines. Osteogenesis is the process of new bone formation results from the transplantation of osteoprogenitor cells along with the growth factors from the bone graft or the host bed. Only autograft materials have osteoblast cells and their precursors. In addition, allografts could combine growth factors, MSCs, osteoprogenitor cells and osteogenic substitutes to provide direct bone development [5-8].
CANCELLOUS BONE GRAFT SUBSTITUTES
Bone grafts are classified according to their sources. Autologous graft (autograft) is described as bone graft material harvested from the host, whereas allogeneic graft (allograft) is defined as the transplant of the graft from one individual to another of the same species with a different genotype [9,10]. Autografts are still remains as the “gold standard” for bone replacement in order to display the best osteoconductive, osteoinductive and osteogenic features. Bone autografts including matrix proteins and osteogenic cells increase bone in growth. They have complete histocompatibility and don’t carry any risk of disease transmission. Although, autologous grafts are the most commonly used materials, they have several disadvantages including additional surgical procedure, donor site morbidity, and significant post-operative complications such as muscle weakness, pain, infection and inadequate amounts of graft material [11].
Substitutes to bone grafting such as DBM, HA, TCP, and their synthetic variants, calcium phosphate and calcium sulfate have been used widely and they have been shown to have osteoconductive but only weakly osteoinductive potential. Growth factors can also be added to provide enhancement of graft incorporation and stimulation of bone healing [12]. DMB is capable of osteoconductive activity to enhance bone formation. Although DMB has no structural strength, it provides rapid vascularization and stable environment for the various proteins and growth factors during demineralization process [13,14]. Urist reported that DBM implants resulted in increased collagen activity. After dissolution of the matrix, new bone formation was observed [15]. Calcium phosphate based ceramics such as HA and TCP are osteo-conductive bone graft substitutes. Chemical structure of HA is similar to mineralized bone matrix and its osteoconductive matrix supports proliferation of bone cells during remodeling process [16]. It provides significant compressive strength but it has a low tensile strength. So, it has limited usage in the therapy of load bearing bone imperfection [17,18]. Even though, porous structure of HA provides osteoconductive property, it doesn’t have enough osteoinductive and osteogenic features.
Calcium based cements such as CaPO4, CaSO4 are generally used as filling materials for their compressive strength but they their tensile strength is lower than that of cancellous bone. Fast absorption rate of calcium based cements causes significant loss of their mechanical properties during their degradation. It is one of the greater limitations particularly for load-bearing applications. While they have been used as biocompatible bone graft substitutes as an expander for osteotomies, insufficient osteo-induction and osteo-integration limit their usage. Several bone derived growth factors and recombinant proteins have been shown to stimulate fracture healing to overcome these disadvantages [19,20].
TCP has been used for a long time as synthetic substitute bone filler in orthopedic and dental surgery. Bioabsorbable, biocompatible and porous structure properties of TCP present excellent timely resorption accompanying in bone remodeling process between 6 and 24 months. TCP has a higher rate of biodegradation than HA after implantation which is occurred by combined osteoclastic resorption and dissolution [21]. However, TCP undergoes reabsorption more rapidly than the host new bone formation resulting inequality between TCP and new bone replacement volumes. For that reason, TCP has been generally used as a filler or expander for autografts [18,22,23].
Growth factors as a bone graft substitutes can be used for the enhancement of the bone graft properties (Table 1).
Table 1: Effects of growth factors on host bone-graft incorporation. |
||
Growth Factors |
Source |
Function |
TGF-β |
Bone extracellular matrix, chondrocytes and platelets |
Stimulates undifferentialed MSCs for osteoblast mitogenesis to regulate collagen and proteoglycan synthesis |
b-FGF |
Macrophages, MSCs osteoblasts and chondrocytes |
Increases osteoblast and chondrocytes cell proliferation and promotes collagen formation |
PDGF |
Endothelial cells, monocytes, platelets, osteoblasts |
Regulate collagen secretion; mitogenetic activity for MSCs and osteoblastic cells |
IGFs |
Chondrocytes, osteoblasts |
Enhance bone remodeling and regulate osteoblast cell proliferation and chondrocyte formation |
VEGF |
Vascular endothetial cells, osteoblasts |
Augments angiogenesis to stimulate mitogenesis of endothelial cells |
BMPs(-2,-4,-7) |
MSCs, osteo-progenitor cells, osteoblasts |
Promote induction of osteoprogenitor cells to turn in to bone forming cells |
Abbreviations: BMPs: Bone Morphogenetic Proteins; PDGFs: Platelets include Platelet Derived Growth Factors; TGF-β: Transforming Growth Factor- beta; VEGF: Vascular Endothelial Growth Factor; IGFs: Insulin-like Growth Factors; b-FGF: Basic Fibroblast Growth Factor; MSCs: Mesenchymal Stem Cells. |
Normally, growth factors are present in bone marrow of the iliac crest and they promote bone stimulation. Autogenous bone marrow aspirate has already been used to stimulate osteogenic repair for delayed union and nonunion of bone defects. Fractured bone starts the cascade of inflammation and clotting cycle which is necessary for bone healing. Researchers have been focused on growth factors to stimulate new bone formation [5,19,24]. BMPs were first reported by Wozney et al. for the treatment of bone defects [25]. Other various subtypes such as BMP-2 and BMP-7 have been discovered in the healing of fractured bones by Tsuji et al. and Makino et al. [26,27]. Although growth factors are strong osteo-inductive agents, they have limitations regarding delivery system. Kaito et.al used combination of synthetic biodegradable porous materials (IP-CHA and PLA-PEG) as a carrier system for delivering rhBMP-2. Results showed that rhBMP-2 containing scaffold system significantly enhanced bone formation [19,28].
Another source of growth factors such as PDGFs, TGF-β, VEGF, EGF and IGFs in bone grafting is the use of autologous PRP. Beyond the pro-coagulant effects of platelets that initiate first response during wound healing process, many growth factors in PRP assist bone tissue healing [29,30].
SOURCES OF CANCELLOUS BONE GRAFT
Autograft is the most commonly used type of bone graft and particularly, cancellous autograft still remains the gold standard for bone regeneration. Autogenous cancellous bone graft is usually used in non-unions with <5-6 cm of bone loss with no strength required. It can be harvested from the ipsilateral extremity during surgery.
Large cancellous graft can be taken from the iliac crest, whereas small amount of graft can be harvested from the proximal tibial metaphysis, distal part of the radius, greater trochanter and medial malleolus of the tibia (Table 2)
Table 2: Sources cancellous bone grafts. |
||
Source |
Indications and Advantages |
Complications and Disadvantages |
Distal end of the tibia |
Used for foot and ankle surgery, easily accessible source of abundant cancellous bone |
Small volume of cancellous graft; joint perforation |
Removal of fibular graft |
For the treatment of large defects such as congenital pseudo arthritis of the tibia |
Mild muscular weakness |
Iliac bone autograft |
Abundant amount of the cancellous bone available; no significant residual disability |
Concerns of donor site morbidity; superficial hematomas and infections |
Distal end of the radius |
Used for hand and upper extremity surgery |
Limited supply; pain and immediate functional disaility in the post operative period |
Greater Trochenter |
Source for surgery of the ipsilateral lower extremity |
Persistent hip pain, morbidity |
Allogeneic cancellous blocks, cubes, chips and particulates |
Bioresorbable, osteoconductive; used for replacement of auto graft to provide structural support; found any size or shape defect for Regeneration osseous defect, filing of bone defects and augmentation of alveolar crest for spinal surgery and general orthopedic reconstruction |
Less potentail fro bone regeneration (compared to auto graft); risk of immunogenicity and possibility of human derived disease transmission. |
[31-33]. Iliac crest is the most commonly used autograft source due to the presence of viable cells, the abundance of growth factors and large surface area of the trabecular architecture [34,35]. However, for the patients who have operation risks or low amount of autograft material, allogeneic cancellous bone graft could be alternative source from a bone bank. They have osteoconductive and osteo-inductive properties and can be found in various shapes and sizes [36,37]. This type of allograft must be screened for any bacterial, fungal and viral infection including HIV and hepatitis. Besides, donor screening, sterile processing, safety of processing technology should be screened by the AATB and FDA to ensure the high quality allografts [38-40]. Although cancellous allografts are alternative sources instead of cancellous autografts, they lack osteogenic properties due to the absence of viable bone cells. Another limitations of allografts are harvesting protocols and storage conditions [19,41].
STRUCTURE OF CANCELLOUS BONE GRAFT
Cancellous autograft structures provide trabecular bone with the osteogenic capacity having for superior osteogenesis under the effect of growth factors and cytokines (Table 3)
Table 3: Properties of cancellous bone grafts. |
|||||
Graft Materials |
Osteogeneis |
Osteoinduction |
Osteoconduction |
Immunogenicity |
Donor Morbidity |
Autologus cancellous graft |
+++ |
++ |
++++ |
- |
+ |
Fresh allogeneic cancellous graft |
- |
+/- |
+ |
++ |
- |
Frozen allogeneic cancellous graft |
- |
+/- |
+ |
- |
- |
Freeze dried allogeneic cancellous Graft |
- |
+/- |
+ |
+/- |
- |
DBM |
- |
++ |
++ |
- |
- |
TCP |
- |
+/- |
+ |
- |
- |
Marking refers to; - no activity; + poor activity; ++, +++ degree of activity; ++++ excellent activity. |
[33]. They are easily re-vascularized and incorporated quickly at the stem cells of the host site since the low oxygen tension and pH of the recipient site attract host pluripotent undifferentiated stem cells to the graft site [42,43]. Although, they have more osteogenic and osteo-inductive capacity than the other graft types, they lack significant mechanical properties. Biomechanical strength during host-graft incorporation depends on biological process of graft osteo-integration. Cancellous autogenous grafts are integrated on a necrotic layer first. Then, the radio-density of the graft initially enhances; but in time, it gradually reduces as the necrotic tissue is resorbed by osteoclast cells. Consequently, mechanical strength of the graft is initially strong, but after the resorption of necrotic bone, in the course of time, structural strength of the graft gradually decreases [11,43].
Allogeneic cancellous grafts can be particulate and have a various shape and size depending on the intended use. They are commercially available and generally have been used for filling of bone defects, spinal fusion augmentation and revision joint reconstructions [11,44]. Cancellous allografts present interconnecting porous system and trabecular architecture which provide a framework for vascular ingrowth and proliferation and differentiation of the cells for bone remodeling at the surgical site [45,46].
BIOLOGY OF CANCELLOUS BONE GRAFT
Autogenous cancellous bone graft
Response of host bone tissue to cancellous bone graft involves a cascade including cellular response, extracellular cellular activity between host bone-graft interfaces until newly formed bone cover the graft surface [34]. Although biology of bonegraft incorporation isn’t very well known, osteogenic process is similar to bone healing mechanism. Initial response starts with the hematoma formation, inflammation, neovascularization and then continues with the gradual focal graft resorption by bone resorption process and bone formation on the graft surfaces regulated by inflammatory cells, cytokines, growth factors and bone tissue cells [47]. Blood cells including platelets, granulocytes and monocytes are the first biological components coming into contact with the cancellous graft. They are infiltrated from the recipient site into the tissue surrounding graft and secrete cytokines and growth factors [42,48].
Hematoma formation and inflammation are preliminary interactions of blood cells. Platelets respond to the foreign surface by the formation of fibrin. Fibrin protein plays a key role as a transitory osteo-conduction matrix for osteogenic cell migration towards surface of the graft. Fibrin deposited graft surface allows formation of the osteoid tissue which then remodels into lamellar bone by migrating osteogenic cells [49,50]. MSCs and osteoblast cells attach to the graft surface and synthesize noncollagenous matrix on it leading to further cell attachment and binding of minerals [51]. In the meantime, fibrous tissue occurs at the recipient site in order to increase the osteoclastic activity. Necrotic tissue in haversian canals of the autogenous cancellous graft is removed by macrophages. It leads to the secretion of intracellular products of the recipient bone site which are chemoattractants for host stem cells. Vascularization process is initiated after 2 days of surgery. Then, MSCs found in both host and graft start to differentiate into osteogenic lineage under the influence of osteo-inductive agents; cytokines and growth factors including TGF-β, PDGF, b-FGF, IGFs and BMPs.
Hematoma formation, inflammation, revascularization and osteo-induction continue as a process together with the bone formation-resorption throughout 4 weeks of surgery [11]. Resorption of the graft by osteoclasts and gradual new bone remodelling on the graft surfaces are the second phase of grafthost bone osteo-integration. After differentiation of MSCs into osteoblasts, they start to lay down a seam of osteoid along the dead trabecula of the bone graft. Necrotic bone tissue is removed by osteoclasts. As a result of osteoclastic activity, graft begins to be resorbed and replaced by new host cells. Finally, MSCs form new bone marrow cells to fill within the old marrow space [33,35,52,53].
Allogeneic cancellous bone grafting
Allogeneic cancellous bone grafts provide an osteoconductive agent for bone regeneration. They are usually used for filling of bone defects, posterior spinal fusions, tibia plateau impression fractures and impaction grafting during femoral or acetabular revisions as structural support [33]. When allogeneic cancellous bone graft is compared with autogeneic cancellous bone graft, less bone healing is obtained in allogeneic bone graft. After transplantation of fresh allogeneic cancellous graft, host mediated immune response by macrophages and lymphocytes leads to inhibition of essential growth factors causing delay of bone-graft incorporation and neo-angiogenesis. Necrosis is occurred by surrounded inflammatory cells. After 8 week of surgery, fibrous tissue starts to cover the cancellous allograft. Unfortunately, aggressive host immune response delay osteointegration of the graft. Freeze - dried or frozen allogeneic cancellous grafts show reduced immunogenicity comparing with the fresh cancellous allograft. However, osteo-conduction and bone resorption of cancellous preserved allografts keep going faster than that of fresh allografts. The transplanted allograft can remain entrapped within the host bone [11,42,53-55].
Freeze- drying method which is the most common preservation procedure of cancellous allografts can destroy the osteo-progenitor cells and osteo-inductive factors, hereby they provide only osteo-conductive properties as a scaffold [56]. For that reason, grafts could combine with the biological elements such as rhBMPs to stimulate cell proliferation, differentiation and osteogenesis [57]. Although positive results were reported, there are also negative feedbacks. Delloye and their colleagues have been evaluated the efficacy of rhBMPs in nonunion of femoral allografts. According to the results, healing of allograft fractures and union of allograft-host junction were not observed. rhBMPs alone were not adequate to treat in allograft non-unions and fractures [58].
Bone allograft causes several histopathological results including avascular necrosis, malignant tumors and osteoarthritis since fatigue micro-fractures are formed in the necrotic bone near the fracture site and it can’t remodel itself resulting in structural failure [59-62]. Osteoclastic resorption of the graft materials, loss of mineral density and unrepaired micro-cracks have been also reported after allograft reconstructions [63].
Prevalence of functional failure of allografts has been noted approximately 60 % at 10 years. The causes of these pathological conditions that influence the allograft incorporation and their effect on physiological and mechanical capacity are still unknown. Various biological process can lead to failure of allograft reconstructions [64]. Moreover, immunological response may also play a part in nonunion at the host–graft junction [65].
DISCUSSION & CONCLUSION
Biological mechanism of cancellous graft involves bone tissue cells, growth factors, cytokines and inflammatory cells in a wellorchestrated manner during re-modelling phase of bone healing [42,66]. Autogenous cancellous bone grafts are considered as the gold standard for bone regeneration, since they present the best osteo-conductive, osteo-inductive and osteogenic properties. Moreover, they provide complete histocompatibility and display minimum immunological rejection [67]. Even though autogenous cancellous bone grafts are effective, they are associated with several restrictions and complications regarding with donorsite morbidity, sufficient quantity of grafts, infections, increased blood loss throughout operation [67,68].
Allogeneic cancellous grafts could be used for reconstruction of the defects. Incorporation of cancellous allograft and revascularization are carried out for almost 8 months after transplantation. Osteogenesis is started by the host cells and host immune response is initiated just after the grafts are transplanted that is followed by the penetration of blood vessels. When revascularization decreases over time, allograft incorporates and necrotic bone is removed. The interface between allograft transplant and host fibro-vascular tissue is the site of osteoclastic activity and bone resorption. The balance between osteolysis and osteogenesis must be maintained for graft incorporation [59-63].
Although allogeneic cancellous graft has several disadvantages including transmission of infection and inability of osteo-conductive property, they are still being used widely in bone defect repair. Even though osteo-inductive agents such as rhBMP-2 might be the candidate to eliminate these limitations, different factors including catabolic proteins secreted by host site cells influence osteo-inductivity. A better understanding of the complex biological mechanism of the host bone-graft osteointegration leads to develop new strategies for the limitations of the usage of cancellous allografts. Therefore, the cascade of complex biological events, including anabolic and catabolic factors should be explored to reduce pathologic results and improve local repair after allograft transplantation
REFERENCES
- Richard E, Senghas MD. Bone and cartilage allografts: biology and clinical applications. J Bone Joint Surg Am. 1991; 73: 957-957.
- Brydone AS, Meek D. Maclaine S. Bone grafting, orthopaedic biomaterials, and the clinical need for bone engineering. Proc Inst Mech Eng H. 2010; 224: 1329-1343.
- Marx RE. Bone and bone graft healing. Oral Maxillofac Surg Clin North Am. 2007; 19: 455-466.
- Greenwald AS, Boden SD, Goldberg VM, Khan Y, Laurencin CT, Rosier RN. Bone-graft substitutes: facts, fictions, and applications. J Bone Joint Surg Am. 2001; 83: 98-103.
- Ilan DI, Ladd AL. Bone graft substitutes. Operative Tech in Plastic & Reconstructive Surg. 2003; 9: 151-160.
- Zorzi AR, Miranda JB. Introduction to bone grafting, 1st edn. China: InTech; 2012.
- Lind M, Bünger C. Factors stimulating bone formation. Eur Spine J. 2001; 10: 102-109.
- Ladd AL, Pliam NB. Use of bone-graft substitutes in distal radius fractures. J Am Acad Orthop Surg. 1999; 7: 279-290.
- Sing H, Levi AD. Bone graft and bone substitute biology. Spine surgery Basics. 2014; 1: 147-152.
- Stevenson S. Biology of bone grafts. Orthop Clin North Am. 1999; 30: 543-552.
- Khan SN, Cammisa FP Jr, Sandhu HS, Diwan AD, Girardi FP, Lane JM. The biology of bone grafting. J Am Acad Orthop Surg. 2005; 13: 77-86.
- Lieberman JR, Friedlaender GE. Bone regeneration and repair. 1st ed. Totowa, NJ: Humana Press Inc. 2005.
- Katz JM, Nataraj C, Jaw R, Deigl E, Bursac P. Demineralized bone matrix as an osteoinductive biomaterial and in vitro predictors of its biological potential. J Biomed Mater Res B ApplBiomater. 2009; 89: 127-134.
- Wang JC, Alanay A, Mark D, Kanim LE, Campbell PA, Dawson EG, et al. A comparison of commercially available demineralized bone matrix for spinal fusion. Eur Spine J. 2007; 16: 1233-1240.
- Urist MR. Bone: formation by autoinduction. Science. 1965; 150: 893- 899.
- Egol KA, Nauth A, Lee M, Pape HC, Watson JT, Borrelli J. Bone Grafting: Sourcing, Timing, Strategies, and Alternatives. J Orthop Trauma. 2015; 29; 12: 10-14.
- Balçik C, Tokdemir T, Senköylü A, Koç N, Timuçin M, Akin S, et al. Early weight bearing of porous HA/TCP (60/40) ceramics in vivo: a longitudinal study in a segmental bone defect model of rabbit. Acta Biomater. 2007; 3: 985-996.
- Moore WR, Graves SE, Bain GI. Synthetic bone graft substitutes. ANZ J Surg. 2001; 71: 354-361.
- Nandi SK, Roy S, Mukherjee P, Kundu B, De DK, Basu D. Orthopaedic applications of bone graft & graft substitutes: a review. Indian J Med Res. 2010; 132: 15-30.
- Costantino PD, Friedman CD. Synthetic bone graft substitutes. Otolaryngol Clin North Am. 1994; 27: 1037-1074.
- Daculsi G, LeGeros RZ, Heughebaert M, Barbieux I. Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics. Calcif Tissue Int. 1990; 46: 20-27.
- Jarcho M. Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop Relat Res. 1981; 259-278.
- Geesink RG, de Groot K, Klein CP. Bonding of bone to apatite-coated implants. J Bone Joint Surg Br. 1988; 70: 17-22.
- Connolly JF. Injectable bone marrow preparations to stimulate osteogenic repair. Clin Orthop Relat Res. 1995; 8-18.
- Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. Novel regulators of bone formation: molecular clones and activities. Science. 1988; 242: 1528-1534.
- Tsuji K, Bandyopadhyay A, Harfe BD, Cox K, Kakar S, Gerstenfeld L, et al. BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. Nat Genet. 2006; 38: 1424-1429.
- Makino T, Hak DJ, Hazelwood SJ, Curtiss S, Reddi AH. Prevention of atrophic nonunion development by recombinant human bone morphogenetic protein-7. J Orthop Res. 2005; 23: 632-638.
- Kaito T, Myoui A, Takaoka K, Saito N, Nishikawa M, Tamai N, et al. Potentiation of the activity of bone morphogenetic protein-2 in bone regeneration by a PLA-PEG/hydroxyapatite composite. Biomaterials. 2005; 26: 73-79.
- El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, Alshahat M, et al. Platelet-rich plasma: growth factors and pro- and anti-inflammatory properties. J Periodontol. 2007; 78: 661-669.
- Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg. 2002; 30: 97-102.
- Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002; 84: 454-464.
- Myeroff C, Archdeacon M. Autogenous bone graft: donor sites and techniques. J Bone Joint Surg Am. 2011; 93: 2227-2236.
- Zorzi AR, Hung NN. Basic knowledge of bone grafting. 1st edn. China: InTech; 2012.
- Fini M, Giavaresi G, Torricelli P, Borsari V, Giardino R, Nicolini A, et al. Osteoporosis and biomaterial osteointegration. Biomed Pharmacother. 2004; 58: 487-493.
- Goldberg VM, Stevenson S. The biology of bone grafts. Semin Arthroplasty. 1993; 4: 58-63.
- Putzier M, Strube P, Funk JF, Gross C, Mönig HJ, Perka C, et al. Allogenic versus autologous cancellous bone in lumbar segmental spondylodesis: a randomized prospective study. Eur Spine J 2009; 18: 687-95.
- Marx RE. Bone and bone graft healing. Oral Maxillofac Surg Clin North Am. 2007; 19: 455-466.
- Centers for Disease Control (CDC). Transmission of HIV through bone transplantation: case report and public health recommendations. MMWR Morb Mortal Wkly Rep. 1988; 37: 597-599.
- Tomford WW. Transmission of disease through transplantation of musculoskeletal allografts. J Bone Joint Surg Am. 1995; 77: 1742-1754.
- Hofmann C, von Garrel T, Gotzen L. Bone bank management using a thermal disinfection system (Lobator SD-1). A critical analysis. Unfallchirurg. 1996; 99: 498-508.
- Habibovic P, de Groot K. Osteoinductive biomaterials--properties and relevance in bone repair. J Tissue Eng Regen Med. 2007; 1: 25-32.
- Mavrogenis AF, Dimitriou R, Parvizi J, Babis GC. Biology of implant osseointegration. J Musculoskelet Neuronal Interact. 2009; 9: 61-71.
- Burchardt H. Biology of bone transplantation. Orthop Clin North Am. 1987; 18: 187-196.
- Smiler D, Soltan M. The bone-grafting decision tree: a systematic methodology for achieving new bone. Implant Dent. 2006; 15: 122- 128.
- Leonetti JA, Koup R. Localized maxillary ridge augmentation with a block allograft for dental implant placement: case reports. Implant Dent. 2003; 12: 217-226.
- Lyford RH, Mills MP, Knapp CI, Scheyer ET, Mellonig JT. Clinical evaluation of freeze-dried block allografts for alveolar ridge augmentation: a case series. The International J of Periodontics & Restorative. 2003; 23: 417-425.
- Goldberg VM, Stevenson S. Natural history of autografts and allografts. Clin Orthop Relat Res. 1987: 7-16.
- Davies JE. Understanding peri-implant endosseous healing. J Dent Educ. 2003; 67: 932-949.
- Pedersen SF, Hoffmann EK, Mills JW. The cytoskeleton and cell volume regulation. Comp Biochem Physiol A Mol Integr Physiol. 2001; 130: 385-399.
- Davies JE. Mechanisms of endosseous integration. Int J Prosthodont. 1998; 11: 391-401.
- Meyer U, Joos U, Mythili J, Stamm T, Hohoff A, Fillies T, et al. Ultrastructural characterization of the implant/bone interface of immediately loaded dental implants. Biomaterials. 2004; 25: 1959- 1967.
- Stevenson S. Biology of bone grafts. Orthop Clin North Am. 1999; 30: 543-552.
- Dumitrescu AL. Chemicals in surgical periodontal therapy. Springer. 2011.
- Boyce T, Edwards J, Scarborough N. Allograft bone. The influence of processing on safety and performance. Orthop Clin North Am. 1999; 30: 571-581.
- Elsalanty ME, Genecov DG. Bone grafts in craniofacial surgery. Craniomaxillofac Trauma Reconstr. 2009; 2: 125-134.
- Habibovic P, de Groot K. Osteoinductive biomaterials--properties and relevance in bone repair. J Tissue Eng Regen Med. 2007; 1: 25-32.
- Faour O, Dimitriou R, Cousins CA, Giannoudis PV. The use of bone graft substitutes in large cancellous voids: any specific needs? Injury. 2011; 42: 87-90.
- Delloye C, Suratwala SJ, Cornu O, Lee FY. Treatment of allograft nonunions with recombinant human bone morphogenetic proteins (rhBMP). Acta Orthop Belg. 2004; 70: 591-597.
- Thompson RC Jr, Pickvance EA, Garry D. Fractures in large-segment allografts. J Bone Joint Surg Am. 1993; 75: 1663-1673.
- Lieberman J, Friedlaender G. Bone regeneration and repair biology and clinical applications. Ann R Coll Surg Engl. 2006; 88: 333-335.
- Ray RD. Vascularization of bone grafts and implants. Clin Orthop Relat Res. 1972; 87: 43-48.
- Beaman FD, Bancroft LW, Peterson JJ, Kransdorf MJ. Bone graft materials and synthetic substitutes. Radiol Clin North Am. 2006; 44: 451-461.
- Beaman FD, Bancroft LW, Peterson JJ, Kransdorf MJ, Menke DM, DeOrio JK. Imaging characteristics of bone graft materials. Radiographics. 2006; 26: 373-388.
- Wheeler DL, Enneking WF. Allograft bone decreases in strength in vivo over time. Clin Orthop Relat Res. 2005: 36-42.
- Hornicek FJ, Gebhardt MC, Tomford WW, Sorger JI, Zavatta M, Menzner JP, et al. Factors affecting nonunion of the allograft-host junction. Clin Orthop Relat Res. 2001: 87-98.
- Linder L, Obrant K, Boivin G. Osseointegration of metallic implants. II. Transmission electron microscopy in the rabbit. Acta Orthop Scand. 1989; 60: 135-139.
- Gazdag AR, Lane JM, Glaser D, Forster RA. Alternatives to Autogenous Bone Graft: Efficacy and Indications. J Am Acad Orthop Surg. 1995; 3: 1-8.
- Summers BN, Eisenstein SM. Donor site pain from the ilium. A complication of lumbar spine fusion. J Bone Joint Surg Br. 1989; 71: 677-680.