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JSM Biotechnology and Biomedical Engineering

Identifying the Fertility Restorers and Maintainers from the Local Landraces in Sorghum

Research Article | Open Access

  • 1. Institut National de la Recherche Agronomique du Niger, Niger
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Corresponding Authors
Aissata MI, Mamadou Ibrahim AISSATA - Institut National de la Recherche Agronomique du Niger (INRAN), BP : 429 Niamey/Niger, Niger, Tel: 227- 96997787
Abstract

Cytoplasmic male sterility system has been widely been used for increasing sorghum yield through heterosis exploitation. To effectively use male–sterility inducing cytoplasm, it is necessary to identify restorers and lines that are suitable for conversion to male sterility. The present study was undertaken to determine the fertility/sterility reactions of a subset of entries from a total of 1019 accessions of two collections (1976 and 2003) of landrace sorghums crossed onto two CMS lines NE223A and Tx623A. The male-sterile lines were Kafir-milo derivatives. Three hundred and forty test-crosses were generated and evaluated in two environments in Niger. Studies revealed four F1 test-crosses (NE223A x L95-1, NE223A x L95-3, NE223A x L119-2 and NE223A x L102- 1) with complete sterility across the two locations. The corresponding parental lines were therefore recommended for conversion to male sterile. However, forty (40) additional test-crosses showed male sterility at Maradi only and two others at Konni respectively, whereas 286 and 272 hybrids shed pollen. The pollen parents of the fertile test crosses were classified as fertility restorers. The male-sterility of female NE223A was maintained by more lines (42) than Tx623A (8) while, at the same time, there were more sterile crosses within the 2003 collection than within the 1976 collection. According to the classification of sorghum races, the sub-group guinea-margaritiferum had the highest number of lines without restorer genes.

Citation

Aissata MI (2018) Identifying the Fertility Restorers and Maintainers from the Local Landraces in Sorghum. JSM Biotechnol Bioeng 5(1): 1085.

Keywords

•    Fertility restorers
•    Cytoplasmic male sterility
•    Sorghum yield

INTRODUCTION

Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop in the world after wheat, rice, maize and barley. In addition to its food, feed and fodder qualities, it has emerged recently as ‘fuel’ crop. Globally, hybrids are the preferred type of varieties in sorghum as they yield 20 to 30% additional grain and stover over a range of environments compared to openpollinated varieties and landraces [1]. However, hybrid cultivar development requires additional research investment over those needed for non-hybrid cultivars for developing suitable parental lines [2].

CMS has been successfully used to develop hybrids for increasing crop production worldwide [3]. The use of cytoplasmic genetic male sterility system in sorghum hybrid development is possible only when effective maintainers and restorers are identified. The introduced CMS lines A/BTx623 and NE223A/B used in the sorghum genetic enhancement program in Niger were those introduced from the US during through institutional collaboration. CMS lines introduced from elsewhere may not be well adapted to a given target environment. Therefore, it is very important to identify maintainers and restorers from local germplasm for development of new lines in a hybrid program. Identification of locally adapted maintainers and restorers which show complete sterility and high degree of restoration of CMS lines would be of great importance in commercial hybrid program.

Cytoplasmic Male Sterility system is a physiological abnormality resulting from disharmonious interaction between the cytoplasmic factors, the mitochondrial genetic factors and nuclear genetic factors leading to the production of degenerate or non-viable pollen grains or non-dehiscent anthers with or without functional pollen grains [2]. Development of a hybrid using CMS requires identification of a male sterile line (A-line), a maintainer line (B-line), and a restorer line (R-line). Main characteristics of these lines are A-line is male sterile, used as female in commercial hybrid production. B-line is isogenic line of A-line, and is needed to maintain A-line. B-line is considered as key line without that A-line could not be maintained. R-line is a male fertile line used as pollen parent in commercial seed production plot. Test cross program help identify maintainers as well as restorers. Maintainer lines used for conversion into new CMS lines and restorer lines are subsequently used as male parent in hybrid development program.

Successful use of hybrid vigor largely depends on the availability of locally developed cytoplasmic genetic male sterile (CMS) and restorer lines [4]. Moreover, breeders need wide range of CMS lines to produce desired hybrids. Therefore, it is necessary to transfer available CMS system into local elite breeding lines. So, the use of local CMS line would be helpful to development of adaptable, heterotic hybrids. Toure and Scheuring [5], evaluated the Malian sorghum collection for sterility/fertility reactions. Potential maintainers and restorers were found and were recommended in the development of A-lines for creation of potential hybrid parents. Efforts have been made by sorghum team in Niger in the early 2000 with the development of L28 A/B line, but some flowers still shed pollen during sterilization phase. The present study was undertaken to identify maintainer and restorer lines from two landrace collection trips conducted in 1976 and 2003 [6,7], where a total of 1019 sorghum accessions were collected.

MATERIALS AND METHODS

Germplasm collection and its description

Two reference sorghum collections were used in this study. The first germplasm collection was done in 1976 by FAO and ORSTOM and covered 183 villages. The second collection was done in 2003 and covered 79 villages of the 183 villages visited in 1976. The visited villages during the collection covered the rainfall pattern and the agro-ecological conditions of the country. The method used and the sampling technique was described by Deu et al., and by Bezancon et al. [6,7]. The genetic diversity of the collection was described by Deu et al. [8].

Germplasm evaluation

The 1019 accessions were evaluated at Maradi and Konni. Maradi is at 07.05 E and 13.48 N and Konni is at 05.05E and 13.48N. Maradi and Konni have approximately the same climate of the Sudano-Sahelien zone. Agro-climatic data (rainfall and temperatures) during the period of evaluation are presented in Figure 1.

ATx623 and AN223 were used as female lines and crossed with local inbred lines used as pollinators. The female ATx623 was introduced from Texas A&M through INRAN/INTSORMIL collaboration in 1984. ATx623 is an early maturing variety with 1.60 m tall, white grain and medium and semi-open panicle. This variety is supposed to be a good combiner. AN223 from the other side is from Nebraska and also introduced through INRAN/ INTSORMIL collaboration. This female with a medium maturity cycle, is dwarf (1m) with long, big and semi-open panicle, with good grain quality. The male parents were local landraces from two collections made in Niger in 1976 and 2003.

F1 production

During the rainy season, the female lines ATx623 and AN223 were crossed with a sample of the two collections at Maradi (longitude 07.05 E; latitude 13.48 N). The samples of each collection were planted separately in bands of one hundred rows. Each row was three meters length. In the middle of the samples were planted the two CMS lines, separately in two bands. The samples of 2003 collected accessions were planted in six bands and the samples of the 1976 in 7 bands. Each band was 100 meters long and comprised 96 varieties with two rows used as borders between bands. Each variety was planted in one row of three meters length and 50 cm between hills. A total of 3 ha land area was used. The trial was supplied with 50 kg/ha NPK 15-15-15 and 50 kg/ha urea (N = 46%) applied twice during the growing season. The field was kept weed free by hand weeding two times during the growing period.

The CMS lines used as females (A lines) receive pollen from those accessions which had good synchronization with them to produce F1 hybrids. The mating was a Line x Tester design, the A lines being used as testers. Staggered planting was used to maximize the number of germplasm collections flowering at the same time as the A lines. For this reason, three different dates of sowing of the female lines were used. Supplementary irrigation was applied to avoid drought stress on the germplasm. Heads of the female parents were covered with pollination bags to avoid contamination with unwanted pollen. An average of three crosses was made between the A lines and each accession.

Method of evaluation

A total of 340 F1 testcrosses obtained from crosses between the local varieties and the two CMS lines were planted during the following rainy season at Maradi with two different dates of sowing and at Konni (one planting date). Each hybrid was planted on a single row of three meters with 50 cm between hills on the same row. The trial was supplied with 50 kg/ha NPK 15-15-15 and 50 kg/ha Urea applied twice during the growing season. The field was kept weed free by hand weeding during the growing period. The methods of evaluation and the selection criteria used were those of Reddy [9].

RESULTS

The 340 F1 hybrids were successfully synthesized (seed obtained) and evaluated the following raining season for sterility/fertility reaction (Table 1).

Evaluation of the 340 F1 in the two localities, 286 and 272 hybrids at Maradi and Konni respectively shed pollen and were classified as fertile. Fifty hybrids (50) were sterile: 44 at Maradi and 6 at Konni. Two sterile accessions obtained in Konni were different from those obtained at Maradi. The sterility of hybrids was confirmed with the lack of pollen productivity during the flowering period and the total absence of grain formation at maturity on the panicles when covered in the two localities. The CMS line AN223 gave 42 of the fifty sterile hybrids and with the ATx623 eight (8) were sterile (Table 2).

Four (4) sterile hybrids (AN223 x L95-1, AN223 x L95-3, AN223 x L102-1, AN223 x L119-2) were common between the two localities. According to the classification of Harlan and De Wet [10], there belong to different races of sorghum (Table 3).

At Maradi, thirty six (36) F1 sterile hybrids (maintainer lines) were obtained with the CMS A- line AN223 and eight (8) sterile hybrids with the CMS A-line ATx623. There were 178 and 108 F1 hybrid fertile (Restorer lines) with each of the two A-lines respectively. Some semi-sterile lines (10) were also observed with the female AN223 (Table 4).

At Konni, only six (6) F1 hybrids all coming from the crosses with the female AN223 were sterile (maintainer lines). There were 272 fertile (Restorer lines) in which 168 with the female AN223 and 104 with the female ATx623. Sixty two (62) other hybrids were affected by drought and couldn’t be evaluated. No semi-sterile F1 hybrid was observed in this locality but 62 F1 hybrids were affected by drought and did not reach the blooming period (Table 5).

During the crossing period, each landrace being a population (family), each cross in the same family is different from another. Evaluating crosses within families, nine of them at Maradi got total sterility in the two repetitions. Eight (8) families were maintainers with the female AN223 and one was maintainer with the female ATx623 (L377). None family got total sterility at Konni. The total sterility within a family implied the sterility of the three crosses that have been made between one tester and the local (Table 6).

When sterile families were partitioned between the different races of sorghum, six of them were Guinea-margaritiferum, one Guinea and one other hybrid Guinea-caudatum. Their complete sterility was obtained with the female AN223. Only one maintainer belonging the natural hybrid Guinea caudatum race was found with the femaleATx623 (Table 7).

DISCUSSION

The four local landraces (L95-1, L95-3, L119-2 and L102- 1) that exhibited completely sterile crosses with the CMS line NE223A at both locations may be converted into CMS lines. Similar results of effective sterility in a national collection were found by Toure and Scheuring [5], at Sotuba and Babougou in Mali. These results meet the requirements of Reddy et al. [11], that demand that a male sterile line used for a large scale production of hybrids should neither shed pollen, nor set seed when selfed, regardless of location or season. For these reasons, the four pollen parents of these sterile hybrids are going to be transformed into A lines through backcrossing. The existence of A lines with local landraces in the breeding program of Niger will improve sorghum production through high yielding hybrids development.

The discovery of complete fertility restorers and complete sterility maintainers within local germplasm is important in Niger’s sorghum breeding program because it helps in the use of landraces in hybrid development. Experimental hybrids were made using local varieties as well as exotic lines as pollinators on seed parents provided by INTSORMIL or ICRISAT. Although well adapted to the environment, most landraces produced tall hybrids which lodged and which are later than their pollinators [12]. There is therefore a need to develop suitable parents and an economical method of hybrid seed production available to small holders [12].

The 280 fertile crosses found differs somewhat from a study in Mali by Toure and Scheuring [13] where out of 227 F1’s evaluated, 142 shed pollen and 86 had non-restorer genes. In this study, only fifty F1 showed male sterility in at least one location and only four were sterile at both locations out of the 340 F1 hybrids evaluated. This implies that most of the germplasm evaluated in Niger restored fertility.

In addition to the crosses that were sterile at one location but fertile at the other, ten crosses were found to be semisterile with the female NE223A at Maradi. These crosses were all discarded from the experiment. This finding is in accordance with Miller and Pickett [14], and Reddy [9], who found that some crosses, were semi-sterile. Male parents that produced semisterile crosses were rejected from a breeding program because they serve neither as restorers nor as maintainers [11]. Toure and Scheuring [13], evaluating local germplasm for stability and fertility reaction in Mali found complete stability across the two environments.

Cytoplasmic male sterility is environmentally dependant. The numbers of non-restorer and restorer lines were higher at Maradi than at Konni. CMS stability in relation to temperature is an important factor in the production of hybrid seeds. Maradi and Konni have approximately the same agro-climatic conditions. Average maximum and minimum temperatures during the crop growing period were at Maradi 37.7°C and 24.7°C, and 38.2°C and 25.9°C at Konni. Total rainfall in the two localities was, respectively, 491.4 and 370.1mm during the experiment. Results agreed with those obtained by Reddy et al. [11]. The difference in rainfall was probably high enough to show the differences in sterility across environments. According to Tarumoto et al. [12], the temperature stimulus in the duration of flower development causes phenotypic fluctuation for sterility in A1 male sterile lines.

Sometimes, during the flowering period when crosses were evaluated for sterility/fertility reactions, there was a risk of contamination which may have affected the crosses. The contamination may be attributed to the fact that covered heads of hybrids were opened for verification of the presence of pollen and at the same time for the evaluation of the anther morphology. This may have resulted in the flowers receiving pollen from other panicles shedding pollen at the time. This probably affected the efficiency of expression of male sterility in some of the hybrids. New methods should therefore be found to facilitate sterility/ fertility evaluation and to render the method more efficient. The proportion of sterile hybrids was 13% at Maradi and 2% at Konni. This proportion may be low according to the numbers of hybrids evaluated (340), but it is a good start for the sorghum program in Niger because it is the first time that this kind of study was conducted.

Three of four sterility maintaining lines (L95-1, L95-3, L119- 2) belong to the guinea margaritiferum race and one (L102-1) is caudatum. The race guinea margaritiferum was found to possess lines that efficiently maintained sterility in the A1 cytoplasm in another study [15]. Backcrossing to transfer genes into the restorer B-lines based on the CMS line NE223A should certainly use this guinea-margaritiferum race however, it is recommended that the sorghum improvement program should place emphasis first on the Caudatum race because it is more productive. Guineamargaritiferum race varieties are found in the Southwest region of the country and are more adapted to high annual rainfall, whereas the Caudatum races are adapted to drier conditions where most sorghum is produced. However, all sorghum basic botanical races except kafir can be found in Niger [16], and each of them can be used for gene transfer in the presence of sterility restorer genes.

The guinea margaritiferum group is genetically differentiated from all other races, including non-margaritiferum guinea sorghums [6,16,17]. This resulted from a lack of concordance in flowering and strong human selection which could contribute to an efficient reproductive barrier [18-21]. The genetic distinctness of margaritiferums from other guinea sorghums from western Africa is remarkable, since both are interfertile and cultivated in sympatry in the same season by the same farmers [16]. The genetic history of guinea-margaritiferum is enigmatic and still requires further investigation within the species Sorghum bicolor [6]. Our study revealed maintainer lines within this race.

CONCLUSION

Four out of 340 F1 hybrids, (~1%) were found with complete sterility leading to the identification of potential A lines from the conversion of 4 Niger landrace sorghums. The 4 landraces are composed of one from the Caudatum race known for adaptation in the dry environments and 3 from the Guinea Margaritefum race adapted for relatively better growing conditions. The use of adapted A lines will enhance hybrid development for high productivity and better adaptation to local conditions. Forty four (44) hybrids exhibited complete sterility at Maradi and six at Konni. However ten others expressed partial sterility a Maradi. In addition, 286 and 272 lines shed pollen and the respective pollen parents were classified as fertility restorers. The large number of fertile hybrids indicates a predominance of R lines among Niger landraces which may be used to derive improved male parents.

Sterilization of lines will continue with the CMS line NE223A as most of the sterile hybrids came from this female. Both NE223A and TX623A may be used for other crosses within the two collections as a great number of the accessions were not successfully crossed and may be sterilized with one of the CMS sources. Following the breeding work with MDK, this is the very first systematic study of the fertility reaction of sorghum landraces in Niger. The diversity in the landraces suggests in itself that farmers in different agro-ecological zones have specific preferences which are in part related to varietal adaptation. Thus this work may lead to the development of various A lines with specific adaptation which will in turn help produce hybrids for the various sorghum growing communities.

REFERENCES

1. Reddy PS, Rao DM, Reddy BVS, Thakur RP, Kumar AA. Agronomic performance of A4 cytoplasmic male sterility system as compared to A1 CMS system of sorghum (Sorghum bicolor). Ind J Agricultural Sci. 2011; 81: 908-913.

2. Reddy BVS, Ramesh S, Sanjana Reddy P. Sorghum breeding research at ICRISAT – Goals, strategies, methods and accomplishments. International Sorghum and Millets Newsletter. 2004; 45: 5-12.

3. Dhillon MK, Sharma HC, Smith CM. Implications of cytoplasmic malesterility systems for development and deployment of pest resistant hybrids in cereals. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 2008; 3.

4. Kumar AA, Reddy BVS, Reddy PS, Ramaiah B. Development of malesterile lines in sorghum. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, Andhra Pradesh, India. 2010.

5. Toure AB, Scheuring JF. Presence des genes maintaineurs de l’androsterilite cytoplasmic parmi les varietes locales de sorgho du Mali. Agronomie Tropicale, XXXVII-4, PP362-365. 1996.

6. Deu M, Sagnard F, Chantereau J, Calatayud C, Herault D, Mariac C, et al. Niger-wide assessment of in situ sorghum genetic diversity with microsatellite markers. Theoretical Applied Genetic. 2008; 116: 903- 916.

7. Bezancon G, Pham JL, Deu M, Vigouroux Y, Sagnard F, Mariac C, et al. Changes in the diversity, geographic distribution of cultivated millet (Pennisetum glaucum [L.] R. Br.), sorghum (Sorghum bicolor (L.) Moench) varieties in Niger between 1976 and 2003. Genetic Resources Crop Evolution. 2009; 56: 223-236.

8. Deu M, Sagnard F, Chantereau J, Calatayud C, Vigouroux Y, Pham JL, et al. Spatio-temporal dynamics of genetic diversity in Sorghum bicolor in Niger. Theoretical Applied Genetics. 2010; 120: 1301-1313.

9. Reddy BVS. Development, production, and maintenance of male sterile lines in sorghum. In: Singh, Faujdar KN. Rai, Belum VS. Reddy, Diwakar B (eds.), Training manual on development of cultivars and seed production techniques in sorghum and pearl millet. ICRISAT, Patancheru, Andhra Pradsh. India. 1997a; 22-27.

10. Harlan JR, de Wet JMJ. A simplified Classification of sorghum. Crop Science. 1972; 12: 172-176.

11. Reddy BVS, Ramesh S, Sanjana Reddy P, Ashok Kumar A. Malesterility inducing cytoplasmic effects on combining ability in sorghum [Sorghum bicolor (L.) Moench]. Ind J Genetics. 2009; 69: 199-204.

12. Tarumoto I, Ishii (Adachi) E, Yanase M, Fujimori M. The Phenotypic Fluctuation Factor for Male Sterility in A1 Male-Sterile Lines of Sorghum (Sorgum bicolor Moench) Sci. Rep. Graduate School of Life &Environmental Science. Osaka Pref. Univ. 2008; 59: 1-6.

13. Scheuring JF, Miller FR. Fertility restorers and sterility maintainers to the milo-kafir genetic cytoplasmic male sterility system in the sorghum world collection. Misc. Publ., Texas A&M Agricultural Experiment Station, College Station, Texas, USA, 1367. 1978.

14. Miller DA, Pickett RC. Inheritance of partial male fertility in Sorghum vulgare Pers. Crop Science. 1964; 4: 1-4.

15. Bosques-Vega A, Sotomayor-Rios A, Torres-Cardona SHR. Maintainer and Restorer with A1, A2, and A3 Cytoplasms of lines from the Sorghum Conversion Program. The Texas Agricultural Experimental Station, Charles J. Arntzen, Acting Director, The Texas A&M University System, College Sation, Texas. 1981.

16. Deu M, Rattunde F, Chantereau J. A global view of genetic diversity in cultivated sorghums using a core collection. Genome. 2006; 49: 168- 180.

17. Deu M, Hamon P, Chantereau J, Dufour P, D’Hont A, Lanaud, C. Mitochondrial DNA diversity in wild and cultivated sorghum. Genome. 1995; 38: 635-645.

18. Guha Sarkar CK, Zaman FU, Singh AK. Genetics of fertility restoration of ‘WA’ based cytoplasmic male sterility system in rice (Oryza sativa L.) using basmati restorer lines. Ind J Genetic. 2002; 62: 305-308.

19. Reddy PS, Rao DM, Reddy BVS, Kumar AA. Inheritance of male-fertility restoration in A1, A2, A3 and A4 (M) cytoplasmic male-sterility systems of sorghum [Sorghum bicolor (L.) Moench]. Ind J Genetic. 2010; 70: 240-246.

20. Reddy BVS, Thakur RP, Ramesh S, Rao VP, Reddy PS. Effcets of cytoplasmic nuclear-male sterility systems on sorghum grain mold development. International Sorghum and Millets Newsletter. 2006; 47: 16-20.

21. Reddy BVS, Ramesh S, Ortiz R. Genetic and cytoplasmic-nuclear malesterility in sorghum. Plant Breeding Rev. 2005; 25: 139-172.

Received : 24 Apr 2018
Accepted : 29 May 2018
Published : 31 May 2018
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ISSN : 2578-3718
Launched : 2016
Annals of Vascular Medicine and Research
ISSN : 2378-9344
Launched : 2014
Journal of Hematology and Transfusion
ISSN : 2333-6684
Launched : 2013
JSM Environmental Science and Ecology
ISSN : 2333-7141
Launched : 2013
Journal of Cardiology and Clinical Research
ISSN : 2333-6676
Launched : 2013
JSM Nanotechnology and Nanomedicine
ISSN : 2334-1815
Launched : 2013
Journal of Ear, Nose and Throat Disorders
ISSN : 2475-9473
Launched : 2016
JSM Ophthalmology
ISSN : 2333-6447
Launched : 2013
Journal of Pharmacology and Clinical Toxicology
ISSN : 2333-7079
Launched : 2013
Annals of Psychiatry and Mental Health
ISSN : 2374-0124
Launched : 2013
Medical Journal of Obstetrics and Gynecology
ISSN : 2333-6439
Launched : 2013
Annals of Pediatrics and Child Health
ISSN : 2373-9312
Launched : 2013
JSM Clinical Pharmaceutics
ISSN : 2379-9498
Launched : 2014
JSM Foot and Ankle
ISSN : 2475-9112
Launched : 2016
JSM Alzheimer's Disease and Related Dementia
ISSN : 2378-9565
Launched : 2014
Journal of Addiction Medicine and Therapy
ISSN : 2333-665X
Launched : 2013
Journal of Veterinary Medicine and Research
ISSN : 2378-931X
Launched : 2013
Annals of Public Health and Research
ISSN : 2378-9328
Launched : 2014
Annals of Orthopedics and Rheumatology
ISSN : 2373-9290
Launched : 2013
Journal of Clinical Nephrology and Research
ISSN : 2379-0652
Launched : 2014
Annals of Community Medicine and Practice
ISSN : 2475-9465
Launched : 2014
Annals of Biometrics and Biostatistics
ISSN : 2374-0116
Launched : 2013
JSM Clinical Case Reports
ISSN : 2373-9819
Launched : 2013
Journal of Cancer Biology and Research
ISSN : 2373-9436
Launched : 2013
Journal of Surgery and Transplantation Science
ISSN : 2379-0911
Launched : 2013
Journal of Dermatology and Clinical Research
ISSN : 2373-9371
Launched : 2013
JSM Gastroenterology and Hepatology
ISSN : 2373-9487
Launched : 2013
TEST Journal of Dentistry
ISSN : 1234-5678
Launched : 2014
Annals of Nursing and Practice
ISSN : 2379-9501
Launched : 2014
JSM Dentistry
ISSN : 2333-7133
Launched : 2013
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