JSM Biotechnology and Biomedical Engineering

RD3: A Challenge and a Promise

Review Article | Open Access

  • 1. Department of Ophthalmology, University of Oklahoma Health Sciences Center (OUHSC), USA
+ Show More - Show Less
Corresponding Authors
Seifollah Azadi, Department of Ophthalmology, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK, 73104, USA

Photoreceptor cells are highly specialized cells that convert light energy into electric signals. Ten percent of their outer segment membranes (approximately 77 cm2 of membrane) are renewed every day. Therefore, photoreceptor cells must possess an extraordinary trafficking system to provide material needed to build up outer segment discs through a 0.3 µm diameter connecting cilium. The mechanism to traffic membrane proteins in the retina and corresponding degenerative diseases is still elusive. The retinal degeneration 3 (Rd3) is the gene responsible for a murine autosomal recessive hereditary retinal degeneration, which is known as Leber Congenital Amaurosis 12 (LCA12). Degeneration of photoreceptor cells begins at about two weeks of age and completes between two and four months. We generated the first antibody against RD3, and through a protein-protein interaction analysis, discovered that the RD3 protein interacts directly with guanylate cyclase 1 (GC1), and partially expresses in the outer segment. We also detected the major binding site between these two proteins and realized that RD3 is directly involved in the trafficking of this crucial protein. In a separate study; we reported that RD3 negatively regulates GC1, which is crucial for efficient trafficking of GC1. During the trafficking path, RD3 prevents unnecessary production of cGMP. It is possible that RD3 may still be involved in regulating GC1 even after targeting. Several mutations that cause visual difficulties have been reported for the mouse and human ortholog of RD3. The symptoms these mutations cause are very similar to those reported for a more severe form of blindness referred to as LCA1. Therefore, RD3 might cause a broader range of retinal diseases. Gene replacement of RD3 has been shown to restore the GC1 across the retina. This makes RD3 a novel therapeutic target for retinal targeting of impaired degenerative diseases.


Azadi S (2013) RD3: A Challenge and a Promise. JSM Biotechnol Bioeng 1(3): 1016.


•    Retina
•    Protein trafficking
•    Retinal degeneration 3
•    Guanylate cyclase1
•    Leber Congenital Amaurosis 1 (LCA1), and LCA12


Malfunctioning of protein targeting in the retina has been linked to a number of retinal degenerative diseases such as retinitis pigmentosa and ciliopathies [1-3]. Nevertheless, the key elements and the pathways, which execute the targeting of membrane proteins, remain elusive [4-7]. One fundamental reason for this obscurity, which has slowed down the progress of the growth of this field and prevents performing developmental studies, is the complexity and uniqueness of the mammalian retina. This is because mammalian photoreceptor cells tolerate drastic qualitative changes such as remodelling, inter segmental fusion, and polarity loss when they are dissociated and therefore generation of an in vitro model for disc renewal and trafficking has not yet been accomplished [8].

Leber Congenital Amaurosis (LCA) is the earliest and most severe form of all inherited retinal dystrophies, characterized by blindness or severe visual impairment from birth. LCA accounts for at least 5% of all retinal dystrophies and is one of the main causes of blindness in children, which is characterized by severe loss in visual function, nystagmus, sluggish pupils and abolished or absent electroretinogram (ERG). So far, 17 different genes have been associated with autosomal recessive forms of LCA. Fundamental cell and molecular mechanisms in photoreceptor cell structure, function, and survival are regulated by these proteins such as phototransduction, vitamin A metabolism, vesicle trafficking, protein assembly, ciliary structure and transport, photoreceptor development and morphogenesis, guanine nucleotide synthesis, and outer segment phagocytosis [9-11]. Currently, no curative or preventative treatments for this debilitating disease exist [10].

Without a doubt, retinal degeneration3 (RD3) is one of the most important findings in vision research in the past decade. A stop codon within Rd3, which causes premature termination of this protein, leads to manifestation of Leber Congenital Amaurosis 12 (LCA12 [12]. This crucial protein, which has been overlooked for a long time, has helped to understand the mechanism of targeting of membrane proteins, particularly guanylate cyclase 1 (GC1). The latter plays a central role in the survival of photoreceptor cells as well as the visual cycle as its function controls the influx of Na2+and Ca2+ into photoreceptor outer segments by associating with cyclic nucleotide-gated channel in the plasma [13]. Loss-of-function mutations in the GC1 gene cause another form of LCA, namely LCA1, which accounts for 20% of all LCA cases [9,10]. The guanylate cyclase activating protein (GCAP1) is a well-known regulator of GC1 activity in an anionic dependent manner [14-16]. We showed that this protein is very much affected by the absence of RD3 as in Rd3-/- retina, GCAP1 is mis-localized to the inner segment [13]. This review will summarize what is currently known about the structure and function of RD3, and we will present an overview of the published and unpublished findings. Moreover, the current understanding of the disease related role of RD3 will be described with an emphasis on broader roles for this protein, which might be involved in other diseases directly related to the malfunction of GC1 in photoreceptor cells.


The Rd3 mutation was originally found among mice captured in Valle di Poschiavo in Switzerland in 1969. The mouse strain carrying this mutation was originally known as “tobacco mouse” [17]. The mice were brought to Jackson Laboratory and were bred into four different lines: RBF/DnJ, RBJ/DnJ, 4Bnr and 30Rk/J. The extent of degeneration among these four genotypes varies significantly [12]. In the Dnj strain, degeneration is very aggressive starting from post natal (P) 14 days, and by three weeks of age, most of the photoreceptors will have been eliminated. The 4Bnr strain has mild degeneration and massive cell death that does not commence before four weeks. The other two genotypes possess even slower rates of degeneration. Moreover, retinal degeneration in pigmented mice is slower than in the albino mice carrying this mutation. Michael Danciger et al. [18] suggested that genetic modifiers contribute to such nonuniformity. For many years RD3, had been overlooked in such a way that it was used for production of hybridoma because the spleen is slightly larger and found to be more efficient for the aforementioned usage [17].


Chang et al. [17] mapped the Rd3 gene to mouse chromosome 1 at 10 ± 2.5 cM distal to Akp1. This was located between markers D1Mit292 and D1Mit51. Due to closeness of Rd3 to USA2A, the field originally considered Rd3 as a mouse ortholog of human USH2A [19]. This gene carries mutations responsible for Usher II a retinal degeneration/hearing loss syndrome. Crossing 4Bnr mice with C57BL/6J mice enabled Danciger et al. [18] to narrow down the location of the Rd3 gene between the markers D1MIT292/ D1MIT209 and D1MIT510, a far enough distance to exclude Rd3 from being a USH2A ortholog. By using meiotic linkage and linkage disequilibrium mapping, Kukekova et al. [20] reduced the map of chromosome 1 to only 230 kb, which contained the three genes TRAF5, C1orf36, and SLC30A. The authors suggested that these genes could be potential positional candidates for rcd2 (rod cone dysplasia type 2). Further analysis showed that the reduced canine rcd2 interval overlapped with murine retinal degeneration 3 (Rd3). Unlike mouse and human Rd3, each has a single known transcript, Kukekova et al. [20] detected three canine retinal Rd3 splice variants and proposed that a sequence alteration was the cause of canine rcd2.


Rd3 mRNA contains 4287 bp of which only 588 bp contains the coding sequence, and the rest is N and C terminal un translated regions. The RD3 gene codes a 195 amino acid protein and produces a 22-23 kDa protein [13]. It contains an N-terminal mitochondrial targeting signal, a possible coiled-coil domain, and two potential phosphorylation sites [21]. Our unpublished data, as well as reports from other groups [21] indicate that Rd3 mRNA is expressed only in the retina. In situ hybridization from our lab performed on adult mouse retina shows strong expression of the Rd3 gene in the inner segment (unpublished data) as well as outer plexiform layer, but Lavorgna et al. [21] showed Rd3 mRNA in the outer nuclear layer, the inner nuclear layer, and the ganglion cell layer. Friedman et al. [12] reported that mouse and human Rd3 has three exons. Using a positional candidate approach, a homozygous transition C to T in c.319 in the third exon of mouse Rd3 causes a premature stop codon at the 107th amino acid, which alters CGA to TGA, thus converting arginine to a stop codon. The same group detected this mutation in all Rd3 mutant lines mentioned above. In the human form of this mutation, however, the stop codon occurs slightly earlier as the 99th amino acid turns out to be a stop codon [12].


The first ever localization reported for RD3 was by Friedman et al. [12], who reported that this protein was associated with leukemia gene product (PML) bodies in the nucleus when they expressed GFP-tagged RD3 in COS1 cells. By expressing Rd3 cDNA in COS1, we detected a different localization than that reported by the mentioned group. We found that RD3 was localized in vesicular structures all across the cytoplasm [13]; however, the addition of GFP has been shown to cause mislocalization [22]. Using acid purification of one of our polyclonal antibodies, we detected the RD3 protein in mouse retina mostly in the outer segment. Our continuous sucrose gradient data supported the immunohistochemistry results, where RD3 was sedimented in the same fractions as the outer segment proteins were. The only conclusion from our localization study is that RD3 is partially localized in the outer segment. We believe the localization of RD3 is not conclusive. Correct localization of this protein is critical and will certainly help to identify its function. Due to the importance of this issue, we have generated several monoclonal and polyclonal antibodies and have set this as a top priority.


In 2006, we prepared five fractions of Rod Outer Segments (total ROSs, ROS membranes, soluble proteins, disk membranes, and enriched plasma membranes) and performed a tandem mass spectrometry-based proteomics approach to identify unknown proteins in rod outer segment [23]. This experiment identified 4,335 peptides from which 516 unique proteins were classified into seven categories according to their known or predicted functions: phototransduction, metabolism, structural, housekeeping, transport, vesicular trafficking, and uncharacterized proteins. An unknown protein was amongst the last category, which at that time was called C1ORF36. One primary reason to investigate RD3 over other proteins was the strong engagement of this protein with the retinal membranes. This was evident from the two following observations: 1) the amino acid composition of RD3 with a low hydrophobicity suggested that RD3 would be soluble, but it was highly insoluble and 2) we were not able to hypotonically release the RD3 from the surrounding membranes. The latter was achievable by lysing the expressed RD3 in 6M urea (data not shown). This data suggests that RD3 is strongly associated with, but not integrated in to the retinal membranes.

The preparation of antibodies against RD3 has always been challenging. The biggest obstacle stemmed from the inability of the N-terminus regions to boost immunogenicity in a mouse. When used, the middle and C-terminus produced antibodies against a nonspecific protein, which was detected by several of our antibodies with a much higher affinity than the conventional RD3 (22 kDa). The second challenge was that RD3, expressed in a bacterial system, was so insoluble that even small pieces of this protein with a Glutathione S-Transferase (GST) tag resulted in bacterial inclusion bodies. In our experience, other tags did not boost detectible immunogenicity against this protein, and thus restricted us to a small protein part for each fusion. We solved this issue by generating a larger number of overlapping fusion proteins. Finally, in the spring of 2009, after a long-term challenge, we generated the first specific monoclonal antibody against this protein [13]. We showed that it directly interacts with GC1 and GC2. GC1 is a membrane protein located in the light-sensitive outer segment of rod and cone photoreceptor cells. In vertebrate rods and cones, photon absorption by rhodopsin or cone visual pigments triggers the hydrolysis of cGMP by activating a transducin-phosphodiesterase 6 (PDE6) cascade, which results in hydrolysis of cGMP and closure of the cGMP-gated cation channels (CNG) in the plasma membrane and membrane hyperpolarization. To recover their function photoreceptor cells must quickly restore the level of cGMP by retinal-specific GC1. Therefore, the function of this enzyme in the production of cGMP is absolutely crucial for phototransduction and the visual cycle. Although the enzymatic properties and activity of GC1 have been studied, the molecular determinants that are responsible for regulation of this activity are incompletely understood. Similarly, even though abnormal targeting of GC1 in the retina underlies several degenerative diseases, the mechanisms of trafficking and outer segment targeting of this protein in photoreceptor cells remains unknown [14,24].

To validate the binding of RD3 and GC1 and to investigate further into the nature of interaction of these two proteins, we co-expressed these two proteins in HEK293 cells and used antibodies against both RD3 and GC1 to perform reverse immunoprecipitation. RD3 and GC1 strongly pulled down one another, which showed that the interaction is likely direct. We next looked into the impact of RD3 on GC1 by co-expressing these two proteins in COS 7 cells. GC1 single transfection resulted in endoplasmic reticulum localization and RD3, when expressed individually, showed a vesicular (punctuated) pattern of expression. Co-expression of these two proteins, however, changed the pattern of expression of these proteins in a way that both proteins turned into round vesicles spreading all across the cytoplasm, which co-labeled with ras-related proteins family member, rab11. Next, we generated deletion mutants of GC1 and co-expressed them with RD3 in HEK 293 cells. Using cyanogen bromide activated beads to conjugate with RD3 antibody and performing immunoprecipitation, we determined the binding site between the two proteins [13]. In a separate publication, [25] to evaluate the impact of RD3 on the activity of GC1, we measured the activity of GC1 in two separate assays. First, the GC1 activity in rod outer segment preparations (containing endogenous GC1 and GCAP1) were measured in the presence of increasing concentrations of 1D4-tagged RD3 purified from bacteria via a 1D4 column. In a subsequent set of experiments, the membrane fraction of hypotonically lysed cells transfected with GC1 was incubated with myristoylated GCAP-His purified from bacteria and RD3-1D4 purified from transfected HEK293 cells. We found that recombinant RD3 expressed in transformed human cell culture or in bacterial cells inhibits GC1/GCAP1 complex activity in HEK293 cell membranes and rod outer segment. This data demonstrates that RD3 is a negative regulator of GC1 activity.

Moreover, our unpublished data suggest that RD3 has another binding site in the cytoplasmic domain of GC1. The binding affinity through this binding site is much less than the main binding site. The latter is called “RD3-GC1 second binding site.” This matter is currently being extensively studied in our laboratory. Together, RD3 regulates the function and trafficking of GC1 through one of the following two models (Figure 1). In model A, RD3 competes with GCAP1 in binding to GC1 therein preventing the activation of GC1. This happens during the trafficking of this complex and dark stage of phototransduction when GC1 remains inactive. In the second model (Figure 1B), RD3 binds to GCAP1, and by conferring conformational changes to this protein, regulates the activity of GC1. In fact, in both models GCAP strongly interacts with RD3, but the nature of this interaction is different in models A and B. It is possible that in the second model, RD3 binds to both GC1 and GCAP1 simultaneously, through the second binding site regulates the activity of this protein, and by the main binding site controls the trafficking of GC1 to the outer segment.

In a recent study by Molday et al. [11], they used an AdenoAssociated Viral Vector (AAV8) with a Y733F capsid mutation containing the mouse RD3 complementary DNA (cDNA), driven by human rhodopsin kinase promoter to subretinaly deliver the Rd3 to photoreceptor cells of 14-day-old of two strains of Rd3 mice; 4Bnr/J and In (5)30Rk/J. Immunofluorescence microscopy was applied to determine whether Rd3 transgene expression restores endogenous GC1 expression and localization to the photoreceptor outer segment layer. Strong GC1 expression was detected in the outer segment of treated mice two weeks postinjection with expression throughout most of the retina. They monitored the prolongation of the GC1 expression in treated retinas and observed the strong GC1 expression for at least seven months. In treated retinas, GCAP1 was restored in the inner segment and to some extent reached the outer segment of photoreceptor cells. To determine the impact of Rd3 delivery on photoreceptor survival, Molday et al. [11] measured the thickness of outer nuclear layer of photoreceptor cells by counting the number of photoreceptor nuclei rows. In healthy retinas, the number of rows in the outer nuclear layer is 10-11. They observed that the treated retinas predominantly have six to eight rows of nuclei. In contrast, the untreated eye was reduced to a single layer. Scotopic and photopic ERG was further used in treated and untreated Rd3-/-mice by the mentioned group to evaluate the impact of AAV-mediated Rd3 delivery on both rod and cone visual function. There was a strong scotopic and photopic response in the treated eye and a significantly reduced response in the untreated eye at 51 days post-injection. They suggested that the aberrant labelling of GC1 in the inner segment is an indication that RD3 is essential for targeting of GC1. This is the in vivo conformation of the work performed by Azadi et al. [13] who initially reported that RD3 traffic GC1 to outer segment. The work performed by Molday et al. [11] is a “proof of concept” for AAV-mediated gene therapy as a potential therapeutic treatment for LCA12, and raises the hope that the diseases caused by the lack of GC1 function can be cured by subretinal gene delivery of Rd3 to the retinal photoreceptor cells.


Friedman et al. [12] performed a mutation analysis on 461 probands with retinopathies and reported an alteration of G to A in exon 2 of Rd3 in one patient, which produced a stop codon at amino acid 100 and resulted in a premature truncation. In the course of screening they found several other alterations: p.W6R, p.E23D, p.K130M. p.G57V, p.W6R/ E23D, p.R68W, p.R167K and p.D195V. They generated p.W6R, p.E23D, p.K130M, and p.W6R; E23D mutations in the RD3-GFP expression construct. The localization of the mutants did not differ from wild type counterpart. We constructed the 6 former mutants and performed a binding analysis with the full-length GC1 when we co-expressed them in HEK293 cells. The binding of GC1 and above mentioned mutants were the same as wild type [25], which confirmed the results from Friedman et al. [12]. It seems that the mentioned mutation can hardly be the cause for the vision difficulties of the probands carrying these mutaions. Perrault et al. [27] performed a comprehensive survey of Rd3 mutations and their clinical expressions in 852 patients affected with LCA or early-onset and severe retinal degeneration. They identified three Rd3 mutations: c.112C.T, which caused p.R38*; c.136G.T, which caused p.E46*; and a 2 bp deletion in c.137–138, which caused a stop codon in amino acid 84. They suggested that the LCA12 phenotype in studied probands were highly similar to those of LCA1. Preising et al. [28] studied retinal degeneration in a consanguineous Kurdish family with LCA/early onset retinal dystrophy. They observed a severe form of retinal dystrophy that showed up at a very early age. Taken together, the mutants carrying defected Rd3 resemble LCA1 and due to overlapping in the mechanism of action, distinguishing these two diseases is not feasible.


Results from our laboratory as well as the data from others have revealed an understanding that RD3 is a very crucial protein for trafficking, regulation, and the manifestation of diseases that involve GC1. The overlapping symptoms in patients carrying Rd3 mutations (LCA12) with LCA1 would raise the possibility that the same mechanism of disease may be the cause of both diseases. In both cases, we believe that the impaired targeting of GC1 is the causal factor of the diseases. We are currently investigating the molecular mechanisms behind the targeting of GC1. The outcome will identify pathways and the key molecules, which execute such a sophisticated mechanism of action. The knowledge from the latter could be adapted for unravelling the trafficking mechanisms of other important photoreceptor membrane proteins.


I would like to gratefully and sincerely thank: 1) Dr. Robert S. Molday for his intellectual input, and never ending support; 2) Dr. Muna I. Naash for her scientific input and her support; and 3) Dr. Robert Eugene Anderson for his support and assistance in revising the manuscript.


My sincerest thanks to the Knight Templar Eye Foundation (KTEF) for supporting this work for two years.


1. Mayhew TM, Astle D. Photoreceptor number and outer segment disk membrane surface area in the retina of the rat: stereological data for whole organ and average photoreceptor cell. J Neurocytol. 1997; 26: 53-61.

2. Rachel RA, Li T, Swaroop A. Photoreceptor sensory cilia and ciliopathies: focus on CEP290, RPGR and their interacting proteins. Cilia. 2012; 1: 22.

3. Deretic D, Mazelova J. Assay for in vitro budding of ciliary-targeted rhodopsin transport carriers. Methods Cell Biol. 2009; 94: 241-257.

4. Deretic D. Post-Golgi trafficking of rhodopsin in retinal photoreceptors. Eye (Lond). 1998; 12 : 526-530.

5. Li L, Khan N, Hurd T, Ghosh AK, Cheng C, Molday R, et al. Ablation of the X-linked retinitis pigmentosa 2 (Rp2) gene in mice results in opsin mislocalization and photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2013; 54: 4503-4511.

6. Nojima S, Toyofuku T, Kamao H, Ishigami C, Kaneko J, Okuno T, et al. A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration. Nat Commun. 2013; 4: 1406.

7. Hollingsworth TJ, Gross AK. Defective trafficking of rhodopsin and its role in retinal degenerations. Int Rev Cell Mol Biol. 2012; 293: 1-44.

8. Sung CH, Chuang JZ. The cell biology of vision. J Cell Biol. 2010; 190: 953-963.

9. Chung DC, Traboulsi EI. Leber congenital amaurosis: clinical correlations with genotypes, gene therapy trials update, and future directions. J AAPOS. 2009; 13: 587-592.

10. den Hollander AI, Roepman R, Koenekoop RK, Cremers FP. Leber congenital amaurosis: genes, proteins and disease mechanisms. Prog Retin Eye Res. 2008; 27: 391-419.

11. Molday LL, Djajadi H, Yan P, Szczygiel L, Boye SL, Chiodo VA, et al. RD3 gene delivery restores guanylate cyclase localization and rescues photoreceptors in the Rd3 mouse model of Leber congenital amaurosis 12. Hum Mol Genet. 2013; 22: 3894-3905.

12. Friedman JS, Chang B, Kannabiran C, Chakarova C, Singh HP, Jalali S, et al. Premature truncation of a novel protein, RD3, exhibiting subnuclear localization is associated with retinal degeneration. Am J Hum Genet. 2006; 79: 1059-1070.

13. Azadi S, Molday LL, Molday RS. RD3, the protein associated with Leber congenital amaurosis type 12, is required for guanylate cyclase trafficking in photoreceptor cells. Proc Natl Acad Sci U S A. 2010; 107: 21158-21163.

14. Hunt DM, Buch P, Michaelides M. Guanylate cyclases and associated activator proteins in retinal disease. Mol Cell Biochem. 2010; 334: 157-168.

15. Dizhoor AM, Olshevskaya EV, Peshenko IV. Mg2+/Ca2+ cation binding cycle of guanylyl cyclase activating proteins (GCAPs): role in regulation of photoreceptor guanylyl cyclase. Mol Cell Biochem. 2010; 334: 117-124.

16. Gorczyca WA, Gray-Keller MP, Detwiler PB, Palczewski K. Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods. Proc Natl Acad Sci U S A. 1994; 91: 4014-4018.

17. Chang B, Heckenlively JR, Hawes NL, Roderick TH. New mouse primary retinal degeneration (rd-3). Genomics. 1993; 16: 45-49.

18. Danciger JS, Danciger M, Nusinowitz S, Rickabaugh T, Farber DB. Genetic and physical maps of the mouse rd3 locus; exclusion of the ortholog of USH2A. Mamm Genome. 1999; 10: 657-661.

19. Pieke-Dahl S, Ohlemiller KK, McGee J, Walsh EJ, Kimberling WJ. Hearing loss in the RBF/DnJ mouse, a proposed animal model of Usher syndrome type IIa. Hear Res. 1997; 112: 1-12.

20. Kukekova AV, Goldstein O, Johnson JL, Richardson MA, Pearce-Kelling SE, Swaroop A, et al. Canine RD3 mutation establishes rod-cone dysplasia type 2 (rcd2) as ortholog of human and murine rd3. Mamm Genome. 2009; 20: 109-123.

21. Lavorgna G, Lestingi M, Ziviello C, Testa F, Simonelli F, Manitto MP, et al. Identification and characterization of C1orf36, a transcript highly expressed in photoreceptor cells, and mutation analysis in retinitis pigmentosa. Biochem Biophys Res Commun. 2003; 308: 414-421.

22. Lisenbee CS, Karnik SK, Trelease RN. Overexpression and mislocalization of a tail-anchored GFP redefines the identity of peroxisomal ER. Traffic. 2003; 4: 491-501.

23. Kwok MC, Holopainen JM, Molday LL, Foster LJ, Molday RS. Proteomics of photoreceptor outer segments identifies a subset of SNARE and Rab proteins implicated in membrane vesicle trafficking and fusion. Mol Cell Proteomics. 2008; 7: 1053-1066.

24. Tucker CL, Ramamurthy V, Pina AL, Loyer M, Dharmaraj S, Li Y, Maumenee IH, et al. Functional analyses of mutant recessive GUCY2D alleles identified in Leber congenital amaurosis patients: protein domain comparisons and dominant negative effects. Mol Vis. 2004; 10: 297-303.

25. Peshenko IV, Olshevskaya EV, Azadi S, Molday LL, Molday RS, Dizhoor AM. Retinal degeneration 3 (RD3) protein inhibits catalytic activity of retinal membrane guanylyl cyclase (RetGC) and its stimulation by activating proteins. Biochemistry. 2011; 50: 9511-9519.

26. Koch KW. Target recognition of guanylate cyclase by guanylate cyclase-activating proteins. Adv Exp Med Biol. 2002; 514: 349-360.

27. Perrault I, Estrada-Cuzcano A, Lopez I, Kohl S, Li S, Testa F, et al. Union makes strength: a worldwide collaborative genetic and clinical study to provide a comprehensive survey of RD3 mutations and delineate the associated phenotype. PLoS One. 2013; 8: e51622.

28. Preising MN, Hausotter-Will N, Solbach MC, Friedburg C, Rüschendorf F, Lorenz B. Mutations in RD3 are associated with an extremely rare and severe form of early onset retinal dystrophy. Invest Ophthalmol Vis Sci. 2012; 53: 3463-3472.

Received : 13 Aug 2013
Accepted : 17 Sep 2013
Published : 19 Sep 2013
Annals of Otolaryngology and Rhinology
ISSN : 2379-948X
Launched : 2014
JSM Schizophrenia
Launched : 2016
Journal of Nausea
Launched : 2020
JSM Internal Medicine
Launched : 2016
JSM Hepatitis
Launched : 2016
JSM Oro Facial Surgeries
ISSN : 2578-3211
Launched : 2016
Journal of Human Nutrition and Food Science
ISSN : 2333-6706
Launched : 2013
JSM Regenerative Medicine and Bioengineering
ISSN : 2379-0490
Launched : 2013
JSM Spine
ISSN : 2578-3181
Launched : 2016
Archives of Palliative Care
ISSN : 2573-1165
Launched : 2016
JSM Nutritional Disorders
ISSN : 2578-3203
Launched : 2017
Annals of Neurodegenerative Disorders
ISSN : 2476-2032
Launched : 2016
Journal of Fever
ISSN : 2641-7782
Launched : 2017
JSM Bone Marrow Research
ISSN : 2578-3351
Launched : 2016
JSM Mathematics and Statistics
ISSN : 2578-3173
Launched : 2014
Journal of Autoimmunity and Research
ISSN : 2573-1173
Launched : 2014
JSM Arthritis
ISSN : 2475-9155
Launched : 2016
JSM Head and Neck Cancer-Cases and Reviews
ISSN : 2573-1610
Launched : 2016
JSM General Surgery Cases and Images
ISSN : 2573-1564
Launched : 2016
JSM Anatomy and Physiology
ISSN : 2573-1262
Launched : 2016
JSM Dental Surgery
ISSN : 2573-1548
Launched : 2016
Annals of Emergency Surgery
ISSN : 2573-1017
Launched : 2016
Annals of Mens Health and Wellness
ISSN : 2641-7707
Launched : 2017
Journal of Preventive Medicine and Health Care
ISSN : 2576-0084
Launched : 2018
Journal of Chronic Diseases and Management
ISSN : 2573-1300
Launched : 2016
Annals of Vaccines and Immunization
ISSN : 2378-9379
Launched : 2014
JSM Heart Surgery Cases and Images
ISSN : 2578-3157
Launched : 2016
Annals of Reproductive Medicine and Treatment
ISSN : 2573-1092
Launched : 2016
JSM Brain Science
ISSN : 2573-1289
Launched : 2016
JSM Biomarkers
ISSN : 2578-3815
Launched : 2014
JSM Biology
ISSN : 2475-9392
Launched : 2016
Archives of Stem Cell and Research
ISSN : 2578-3580
Launched : 2014
Annals of Clinical and Medical Microbiology
ISSN : 2578-3629
Launched : 2014
JSM Pediatric Surgery
ISSN : 2578-3149
Launched : 2017
Journal of Memory Disorder and Rehabilitation
ISSN : 2578-319X
Launched : 2016
JSM Tropical Medicine and Research
ISSN : 2578-3165
Launched : 2016
JSM Head and Face Medicine
ISSN : 2578-3793
Launched : 2016
JSM Cardiothoracic Surgery
ISSN : 2573-1297
Launched : 2016
JSM Bone and Joint Diseases
ISSN : 2578-3351
Launched : 2017
JSM Bioavailability and Bioequivalence
ISSN : 2641-7812
Launched : 2017
JSM Atherosclerosis
ISSN : 2573-1270
Launched : 2016
Journal of Genitourinary Disorders
ISSN : 2641-7790
Launched : 2017
Journal of Fractures and Sprains
ISSN : 2578-3831
Launched : 2016
Journal of Autism and Epilepsy
ISSN : 2641-7774
Launched : 2016
Annals of Marine Biology and Research
ISSN : 2573-105X
Launched : 2014
JSM Health Education & Primary Health Care
ISSN : 2578-3777
Launched : 2016
JSM Communication Disorders
ISSN : 2578-3807
Launched : 2016
Annals of Musculoskeletal Disorders
ISSN : 2578-3599
Launched : 2016
Annals of Virology and Research
ISSN : 2573-1122
Launched : 2014
JSM Renal Medicine
ISSN : 2573-1637
Launched : 2016
Journal of Muscle Health
ISSN : 2578-3823
Launched : 2016
JSM Genetics and Genomics
ISSN : 2334-1823
Launched : 2013
JSM Anxiety and Depression
ISSN : 2475-9139
Launched : 2016
Clinical Journal of Heart Diseases
ISSN : 2641-7766
Launched : 2016
Annals of Medicinal Chemistry and Research
ISSN : 2378-9336
Launched : 2014
JSM Pain and Management
ISSN : 2578-3378
Launched : 2016
JSM Women's Health
ISSN : 2578-3696
Launched : 2016
Clinical Research in HIV or AIDS
ISSN : 2374-0094
Launched : 2013
Journal of Endocrinology, Diabetes and Obesity
ISSN : 2333-6692
Launched : 2013
Journal of Substance Abuse and Alcoholism
ISSN : 2373-9363
Launched : 2013
JSM Neurosurgery and Spine
ISSN : 2373-9479
Launched : 2013
Journal of Liver and Clinical Research
ISSN : 2379-0830
Launched : 2014
Journal of Drug Design and Research
ISSN : 2379-089X
Launched : 2014
JSM Clinical Oncology and Research
ISSN : 2373-938X
Launched : 2013
JSM Bioinformatics, Genomics and Proteomics
ISSN : 2576-1102
Launched : 2014
JSM Chemistry
ISSN : 2334-1831
Launched : 2013
Journal of Trauma and Care
ISSN : 2573-1246
Launched : 2014
JSM Surgical Oncology and Research
ISSN : 2578-3688
Launched : 2016
Annals of Food Processing and Preservation
ISSN : 2573-1033
Launched : 2016
Journal of Radiology and Radiation Therapy
ISSN : 2333-7095
Launched : 2013
JSM Physical Medicine and Rehabilitation
ISSN : 2578-3572
Launched : 2016
Annals of Clinical Pathology
ISSN : 2373-9282
Launched : 2013
Annals of Cardiovascular Diseases
ISSN : 2641-7731
Launched : 2016
Journal of Behavior
ISSN : 2576-0076
Launched : 2016
Annals of Clinical and Experimental Metabolism
ISSN : 2572-2492
Launched : 2016
Clinical Research in Infectious Diseases
ISSN : 2379-0636
Launched : 2013
JSM Microbiology
ISSN : 2333-6455
Launched : 2013
Journal of Urology and Research
ISSN : 2379-951X
Launched : 2014
Journal of Family Medicine and Community Health
ISSN : 2379-0547
Launched : 2013
Annals of Pregnancy and Care
ISSN : 2578-336X
Launched : 2017
JSM Cell and Developmental Biology
ISSN : 2379-061X
Launched : 2013
Annals of Aquaculture and Research
ISSN : 2379-0881
Launched : 2014
Clinical Research in Pulmonology
ISSN : 2333-6625
Launched : 2013
Journal of Immunology and Clinical Research
ISSN : 2333-6714
Launched : 2013
Annals of Forensic Research and Analysis
ISSN : 2378-9476
Launched : 2014
JSM Biochemistry and Molecular Biology
ISSN : 2333-7109
Launched : 2013
Annals of Breast Cancer Research
ISSN : 2641-7685
Launched : 2016
Annals of Gerontology and Geriatric Research
ISSN : 2378-9409
Launched : 2014
Journal of Sleep Medicine and Disorders
ISSN : 2379-0822
Launched : 2014
JSM Burns and Trauma
ISSN : 2475-9406
Launched : 2016
Chemical Engineering and Process Techniques
ISSN : 2333-6633
Launched : 2013
Annals of Clinical Cytology and Pathology
ISSN : 2475-9430
Launched : 2014
JSM Allergy and Asthma
ISSN : 2573-1254
Launched : 2016
Journal of Neurological Disorders and Stroke
ISSN : 2334-2307
Launched : 2013
Annals of Sports Medicine and Research
ISSN : 2379-0571
Launched : 2014
JSM Sexual Medicine
ISSN : 2578-3718
Launched : 2016
Annals of Vascular Medicine and Research
ISSN : 2378-9344
Launched : 2014
Journal of Hematology and Transfusion
ISSN : 2333-6684
Launched : 2013
JSM Environmental Science and Ecology
ISSN : 2333-7141
Launched : 2013
Journal of Cardiology and Clinical Research
ISSN : 2333-6676
Launched : 2013
JSM Nanotechnology and Nanomedicine
ISSN : 2334-1815
Launched : 2013
Journal of Ear, Nose and Throat Disorders
ISSN : 2475-9473
Launched : 2016
JSM Ophthalmology
ISSN : 2333-6447
Launched : 2013
Journal of Pharmacology and Clinical Toxicology
ISSN : 2333-7079
Launched : 2013
Annals of Psychiatry and Mental Health
ISSN : 2374-0124
Launched : 2013
Medical Journal of Obstetrics and Gynecology
ISSN : 2333-6439
Launched : 2013
Annals of Pediatrics and Child Health
ISSN : 2373-9312
Launched : 2013
JSM Clinical Pharmaceutics
ISSN : 2379-9498
Launched : 2014
JSM Foot and Ankle
ISSN : 2475-9112
Launched : 2016
JSM Alzheimer's Disease and Related Dementia
ISSN : 2378-9565
Launched : 2014
Journal of Addiction Medicine and Therapy
ISSN : 2333-665X
Launched : 2013
Journal of Veterinary Medicine and Research
ISSN : 2378-931X
Launched : 2013
Annals of Public Health and Research
ISSN : 2378-9328
Launched : 2014
Annals of Orthopedics and Rheumatology
ISSN : 2373-9290
Launched : 2013
Journal of Clinical Nephrology and Research
ISSN : 2379-0652
Launched : 2014
Annals of Community Medicine and Practice
ISSN : 2475-9465
Launched : 2014
Annals of Biometrics and Biostatistics
ISSN : 2374-0116
Launched : 2013
JSM Clinical Case Reports
ISSN : 2373-9819
Launched : 2013
Journal of Cancer Biology and Research
ISSN : 2373-9436
Launched : 2013
Journal of Surgery and Transplantation Science
ISSN : 2379-0911
Launched : 2013
Journal of Dermatology and Clinical Research
ISSN : 2373-9371
Launched : 2013
JSM Gastroenterology and Hepatology
ISSN : 2373-9487
Launched : 2013
Annals of Nursing and Practice
ISSN : 2379-9501
Launched : 2014
JSM Dentistry
ISSN : 2333-7133
Launched : 2013
Author Information X