Occurrence and Health Risk Assessment of Tropane Alkaloids (Atropine and Scopolamine) in Cereal-Based Foods Consumed by Children and Adults in N’djamena, Chad - Abstract
Tropane alkaloids (TAs), mainly atropine and scopolamine, are toxic, plant-derived substances produced as secondary metabolites and which are primarily found in Datura
Stramonium spp, belonging to the Solanaceae family. TAs’ dietary exposure can lead to harmful health effects, including anticholinergic toxicity, which can be life-threatening.
This study aimed to find out the occurrence of TAs in cereal-based foods and evaluate the potential health risks associated with their consumption amongst children and adults
in N’djamena, Chad. TAs were analyzed in 40 food samples collected from different households for the presence and levels of scopolamine and atropine using separate ELISA
test kits. Findings showed that, the studied cereal-based food samples were contaminated with atropine and scopolamine at varied mean levels [millet (atropine: 2,417 µg/kg;
scopolamine: 0.927 µg/kg), maize (atropine: 2,573 µg/kg; scopolamine: 0.779 µg/kg), and pearl millet (atropine: 2,515 µg/kg; scopolamine: 0.854 µg/kg)] with mean atropine
levels exceeding the EU’s maximum limit (ML) of 1 µg/kg; as opposed to 25% (10/40) samples with mean scopolamine levels exceeding the EU’s maximum limit of 1 µg/kg. These
findings indicate a potential health risk for consumers: children and adults who regularly consume these cereal-based foods in N’djamena, Chad. Considering the estimated daily
intake for children (0.07 µg/kg/day) and adults (0.132 µg/kg/day), and a Benchmark Dose Level (BMDL) at 1.54 µg/kg, the calculated Margin of Exposure (MOE) values were 22
for children and 11.66 for adults, implying the toxic contaminant is a public health concern, thus exposes consumer populations to associated health risks.